Abstract

Cell cycle, DNA replication, repair, and recombination in the dimorphic human pathogenic fungus Paracoccidioides brasiliensis

Author(s): Viviane Castelo Branco Reis, Fernando Araripe Gon�§alves Torres, Marcio Jos�© Po�§as-Fonseca, Marlene Teixeira De-Souza, Diorge Paulo de Souza, Jo�£o Ricardo Moreira Almeida, Camila Marinho-Silva, N�¡dia Skorupa Parachin, Alessandra da Silva Dantas, Thiago Machado Mello-de-Sousa and L�­dia Maria Pepe de Moraes

DNA replication, together with repair mechanisms and cell cycle control, are the most important cellular processes necessary to maintain correct transfer of genetic information to the progeny. These processes are well conserved throughout the Eukarya, and the genes that are involved provide essential information for understanding the life cycle of an organism. We used computational tools for data mining of genes involved in these processes in the pathogenic fungus Paracoccidiodes brasiliensis. Data derived from transcriptome analysis revealed that the cell cycle of this fungus, as well as DNA replication and repair, and the recombination machineries, are highly similar to those of the yeast Saccharomyces cerevisiae. Among orthologs detected in both species, there are genes related to cytoskeleton structure and assembly, chromosome segregation, and cell cycle control genes. We identified atleast one representative gene from each step of the initiation of DNA replication. Major players in the process of DNA damage and repair were also identified.

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