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Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Author(s): E.T. Werner, T.C.B. Soares, A.B.P.L. Gontijo, J.D. Souza Neto and J.A.T. do Amaral

Crambe (Crambe abyssinica) is a non-edible annual herb, which was first cultivated to extract oil for industry, and now has great potential for biodiesel production. The objective of this investigation was to evaluate the genetic stability of micropropagated plants of the C. abyssinica Hochst cultivar ‘FMS brilhante’ using polymerase chain reaction techniques based on inter-simple sequence repeat (ISSR) molecular markers. The aim was to develop a protocol for the in vitro regeneration of these plants with low genetic variation as compared to the donor plant