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Identification of sunflower (Helianthus annuus, Asteraceae) hybrids using simple-sequence repeat markers

Author(s): A. Iqbal, H.A. Sadaqat, A.S. Khan and M. Amjad

Hybrid identification of 16 sunflower hybrids was confirmed using simple-sequence repeat methodology. Of 20 specific simple-sequence repeat primers, 18 authenticated the purity of these hybrids; the remaining two specific primer pairs gave ambiguous DNA fragments. The results indicate that simple-sequence repeat analysis for the identification of hybrids derived from the crossing of different inbred sunflower lines can improve the accuracy of selection, save time and reduce cost.