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A.A. Alfieri

Publications of : A.A. Alfieri
Animal Genetics   Research Article

Brazilian strain of bovine respiratory coronavirus is derived from dual enteric and respiratory tropism

Bovine coronavirus (BCoV) is a pathogen related to enteric and respiratory diseases in cattle worldwide. Enteric (BECoV) strains of BCoV are predominant in South America, and genetic investigations have been conducted to identify its relationship with isolates of respiratory origin (BRCoV). In this study, we used a BRCoV strain (BR-UEL11) derived from an outbreak of.. Read More»

Genet. Mol. Res. 16(2): gmr16029580

DOI: 10.4238/gmr16029580

Microbial Genetics   Research Article

VP6 gene diversity in Brazilian strains of porcine group C rotavirus

    K.C. Médici, A.F. Barry, A.F. Alfieri and A.A. Alfieri

Group C rotavirus (RV-C) has been found in Brazilian pig herds; however, wild-type strains have not yet been characterized. We made a molecular analysis of a region of gene 5 in Brazilian RV-C strains. Stool samples from 11 piglets (diarrheic and with normal consistency) positive for the RV-C VP6 gene in an RT-PCR assay were sequenced. A 270-bp amplicon of nine sequ.. Read More»

Genet. Mol. Res. 9(1): vol9-1gmr715

DOI: 10.4238/vol9-1gmr715

Medical Genetics   Research Article

Phylogenetic analyses of the hemagglutinin gene of wild-type strains of canine distemper virus in southern Brazil

    F.J. Negrão, N.R. Gardinali, S.A. Headley, A.A. Alfieri, M.A. Fernandez and A.F. Alfieri

This study examined the phylogenetic relationship of strains of canine distemper virus (CDV) collected from Paraná State, Brazil, based on the hemagglutinin gene. Urine samples were collected from 4 dogs from northern Paraná State that demonstrated clinical manifestations of canine distemper. The participation of CDV was initially confirmed by RT-PCR targeting the nucleocapsid pro.. Read More»

Genet. Mol. Res. 12(3): 2013.March.11.2

DOI: 10.4238/2013.March.11.2

Animal Genetics   Research Article

Isolation and molecular characterization of Leptospira borgpetersenii serovar Hardjo strain Hardjobovis in the urine of naturally infected cattle in Brazil

    R.T. Chideroli1, U.P. Pereira2, D.D. Gonçalves3, A.Y. Nakamura4, A.A. Alfieri5, A.F. Alfieri5 and J.C. Freitas1

Most epidemiologic studies on bovine leptospirosis are based on serological tests that use antibodies against several serotypes, including the serovar Hardjo, which is widespread and considered to be the most adapted to bovine hosts. However, using only serological studies is not sufficient to identify and distinguish species of leptospires. The aim of this study was report the first isolation .. Read More»

Genet. Mol. Res. 15(1): gmr.15018473

DOI: 10.4238/gmr.15018473

Plant Genetics   Research Article

Effect of propolis ethanol extract on myostatin gene expression and muscle morphometry of Nile tilapia in net cages

    E.L. Buck, I.Y. Mizubuti, A.A. Alfieri, R.A.A. Otonel, L.Y. Buck,F.P. Souza, O.P. Prado-Calixto, A.R. Poveda-Parra, L. Alexandre Filho, N.M. Lopera-Barrero

Propolis can be used as growth enhancer due to its antimicrobial, antioxidant, and immune-stimulant properties, but its effects on morphometry and muscle gene expression are largely unknown. The present study evaluates the influence of propolis on muscle morphometry and myostatin gene expression in Nile tilapia (Oreochromis niloticus) bred in net cages. Reversed mal.. Read More»

Genet. Mol. Res. 16(1): gmr16019404

DOI: 10.4238/gmr16019404

Animal Genetics   Research Article

Morphology and muscle gene expression in GIFT and Supreme Nile tilapia varieties reared in two cultivation systems

    E.C.S. Lima, J.A. Povh, R.A.A. Otonel, J.H. Leonhardt, A.A. Alfieri, S.A. Headley, F.P. Souza, A.R. Poveda-Parra, P.J. Furlan-Murari, N.M. Lopera-Barrer1

Tissue growth in most fishes occurs by muscular hyperplasia and hypertrophy, which are influenced by different regulatory factors, such as myostatin. The current study evaluated the influence of cultivation in hapas and earthen ponds on the diameter of white muscle fibers and on the myostatin (MSTN-1) gene in GIFT and Supreme varieties of tilapia. Fish of both varie.. Read More»

Genet. Mol. Res. 16(1): gmr16019407

DOI: 10.4238/gmr16019407