A.C. Freitas

Synergic effect of oral contraceptives, GSTP1 polymorphisms, and high-risk HPV infection in development of cervical lesions

• B.S. Chagas
• A.P.A.D. Gurgel
• S.S.L. Paiva JÃ?ºnior
• R.C.P. Lima
• M.N. Cordeiro
• R.R. Moura
• A.V.C. Coelho
• K.C.G. Nascimento
• J.C. Silva Neto
• S. Crovella
• A.C. Freitas

Human papillomavirus (HPV) infection is considered a risk factor for cervical cancer. Even if the high-risk HPV (HR-HPV) infection is necessary, environmental co-factors and genetic susceptibility also play an important role in cervical cancer development. In this study, a possible association of rs1695 GSTP1 polymorphisms, HR-HPV infection, and oral contraceptive u.. Read More»

Genet. Mol. Res. 16(3): gmr16039742

DOI: 10.4238/gmr16039742

Detection and expression of bovine papillomavirus in blood of healthy and papillomatosis-affected cattle

M.A.R. Silva, B.M.F. De Albuquerque, N.E. Pontes, L.C.A. Coutinho, M.C.G. Leitão, M.C. Reis, R.S. Castro and A.C. Freitas

Papillomaviruses (PV) are double-stranded DNA viruses that can cause benignant and malignant tumors in amniotes. There are 13 types of bovine papillomavirus (BPV-1 to -13); they have been found in reproductive tissues and body fluids. Normally these viruses are detected in epithelial tissue. We looked for BPV in the blood of healthy cattle and cattle with papillomatosis, using PCR and RT-PCR. B.. Read More»

Genet. Mol. Res. 12(3): 2013.February.28.31

DOI: 10.4238/2013.February.28.31

Polymorphism in the A2M gene associated with high-quality milk in Murrah buffaloes (Bubalus bubalis)

A.C. Freitas, G.M.F. de Camargo, R.R. Aspilcueta-Borquis, N.B. Stafuzza, G.C. Venturini, F. Tanamati, N.A. Hurtado-Lugo, C.C. Barros and H. Tonhati

The study of genes associated with host defense mechanisms, such as the A2M gene, plays a critical role in preventing diseases that reduce milk yield and its constituents. The aim of this study was to identify polymorphisms in the A2M gene in Murrah buffaloes (Bubalus bubalis), and investigate their associations with milk yield, fat and protein production, fat and p.. Read More»

Genet. Mol. Res. 15(2): gmr.15027637

DOI: 10.4238/gmr.15027637

Frequency of human papillomavirus types 16, 18, 31, and 33 and sites of cervical lesions in gynecological patients from Recife, Brazil

M.F.P.T. Baldez da Silva, V. Guimarães, M.A.R. Silva, C.M. Medeiros do Amaral, W. Beçak, R.C. Stocco, A.C. Freitas and S. Crovella

Human papilloma virus (HPV) is a well-established cause of cervical cancer. While many studies have been performed so far on HPV viral biology, mode of infection and prevention measures, scanty information is available on lesion sites of infected women and the incidence of viral types at specific locations. We looked for a possible relationship between the most comm.. Read More»

Genet. Mol. Res. 11(1): 2012.March.1.3

DOI: 10.4238/2012.March.1.3

Simultaneous presence of bovine papillomavirus and bovine leukemia virus in different bovine tissues: in situ hybridization and cytogenetic analysis

A. Yaguiu, M.L.Z. Dagli, E.H. Birgel Jr., B.C.A. Alves Reis,O.P. Ferraz, E.M. Pituco6, A.C. Freitas, W. Beçak and R.C. Stocco

Bovine papillomavirus (BPV) DNA sequences were detected in different tissues, in addition to epithelium. Cytogenetic abnormalities were observed in blood lymphocytes. The presence of more than one virus in a single tissue is a difficult aspect to evaluate,especially when the DNA sequences are detected in tissues that are not specifically targeted by the virus. BPV a.. Read More»

Genet. Mol. Res. 7(2):

Bovine papillomavirus DNA in milk, blood, urine, semen, and spermatozoa of bovine papillomavirus-infected animals

C.J. Lindsey M.E. Almeida C.F. Vicari C. Carvalho A. Yaguiu A.C. Freitas W. Beçak R.C. Stocco

Papillomavirus infection in bovines is associated with cutaneous papillomatosis on the hide, udders and other epithelial tissues, as well as in oral respiratory, alimentary and urinary tract mucosa. Bovine papillomavirus (BPV) is also considered the etiological agent of esophageal tumors and the malignant bladder tumors that characterize the clinical condition assoc.. Read More»

Genet. Mol. Res. 8(1): vol8-1gmr573

DOI: 10.4238/vol8-1gmr573

Simultaneous presence of bovine papillomavirus in blood and in short-term lymphocyte cultures from dairy cattle in Pernambuco, Brazil

N. Diniz, T.C. Melo, J.F. Santos, E. Mori, P.E. Brandão, L.J. Richtzenhain, A.C. Freitas, W. Beçak, R.F. Carvalho and R.C. Stocco

Bovine papillomaviruses (BPV) are the causal agents of benign and malignant lesions; they can cause dramatic economic losses in cattle. Although 10 virus types have been described, three types are most common in tumors, namely BPV-1, -2 and -4. Previous studies have reported BPV in blood cells and the possibility of blood acting as a latent virus site and/or transmi.. Read More»

Genet. Mol. Res. 8(4): vol8-4gmr668

DOI: 10.4238/vol8-4gmr668

HPV31 and HPV33 incidence in cervical samples from women in Recife, Brazil

M.F.P.T. Baldez da Silva, B.S. Chagas, V. Guimarães, L.M.C. Katz,P.M. Felix, P.M. Miranda, A.A. Lima, L.C. Arraes, D.B.G. Martins, J.L. Lima Filho, R.C. Stocco, S. Crovella,7, A.C. Freitas and W. Beçak

Human papillomavirus (HPV) has been extensively studied concerning genomic structure, infection mechanisms, and diversity of types, as well as disease progression stages and development of vaccines. HPV type prevalence can differ in specific populations in different countries, according to ethnicity. This is the first report of an integrated project to evaluate the .. Read More»

Genet. Mol. Res. 8(4): vol8-4gmr677

DOI: 10.4238/vol8-4gmr677

Expression of the bovine papillomavirus type 1, 2 and 4 L1 genes in the yeast Pichia pastoris

A.L.S. Jesus, F.C. Mariz, H.M. Souza, M.N. Cordeiro, E.C. Coimbra, M.C.G. Leitão, L.M. Nascimento, R.C. Stocco, W. Beçak and A.C. Freitas

Papillomaviruses are known to cause benign or malignant lesions in various animals. In cattle, bovine papillomavirus (BPV) is the etiologic agent of papillomatosis and neoplasia of the upper gastrointestinal tract and urinary bladder. Currently, there are no standard diagnostic tests or prophylactic vaccines. Protection against papillomavirus infection is conferred by neutralizing antibodies di.. Read More»

Genet. Mol. Res. 11(3): 2012.July.19.2

DOI: 10.4238/2012.July.19.2

Association between single-nucleotide polymorphisms and milk production traits in buffalo

G.C. Venturini1, D.F. Cardoso1, F. Baldi1, A.C. Freitas1, R.R. Aspilcueta-Borquis1, D.J.A. Santos1, G.M.F. Camargo1, N.B. Stafuzza2, L.G. Albuquerque1 and H. Tonhati1

The aim of this study was to identify single-nucleotide polymorphisms (SNPs) in buffaloes associated with milk yield and content, in addition to somatic cell scores based on the cross-species transferability of SNPs from cattle to buffalo. A total of 15,745 SNPs were analyzed, of which 1562 showed 1% significance and 4742 with 5% significance, which were associated for all traits studied. After.. Read More»

Genet. Mol. Res. 13(4): 2014.December.4.20

DOI: 10.4238/2014.December.4.20