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A.F. Alfieri

Publications of : A.F. Alfieri
Animal Genetics   Research Article

Brazilian strain of bovine respiratory coronavirus is derived from dual enteric and respiratory tropism

Bovine coronavirus (BCoV) is a pathogen related to enteric and respiratory diseases in cattle worldwide. Enteric (BECoV) strains of BCoV are predominant in South America, and genetic investigations have been conducted to identify its relationship with isolates of respiratory origin (BRCoV). In this study, we used a BRCoV strain (BR-UEL11) derived from an outbreak of.. Read More»

Genet. Mol. Res. 16(2): gmr16029580

DOI: 10.4238/gmr16029580

Microbial Genetics   Research Article

VP6 gene diversity in Brazilian strains of porcine group C rotavirus

    K.C. Médici, A.F. Barry, A.F. Alfieri and A.A. Alfieri

Group C rotavirus (RV-C) has been found in Brazilian pig herds; however, wild-type strains have not yet been characterized. We made a molecular analysis of a region of gene 5 in Brazilian RV-C strains. Stool samples from 11 piglets (diarrheic and with normal consistency) positive for the RV-C VP6 gene in an RT-PCR assay were sequenced. A 270-bp amplicon of nine sequ.. Read More»

Genet. Mol. Res. 9(1): vol9-1gmr715

DOI: 10.4238/vol9-1gmr715

Medical Genetics   Research Article

Phylogenetic analyses of the hemagglutinin gene of wild-type strains of canine distemper virus in southern Brazil

    F.J. Negrão, N.R. Gardinali, S.A. Headley, A.A. Alfieri, M.A. Fernandez and A.F. Alfieri

This study examined the phylogenetic relationship of strains of canine distemper virus (CDV) collected from Paraná State, Brazil, based on the hemagglutinin gene. Urine samples were collected from 4 dogs from northern Paraná State that demonstrated clinical manifestations of canine distemper. The participation of CDV was initially confirmed by RT-PCR targeting the nucleocapsid pro.. Read More»

Genet. Mol. Res. 12(3): 2013.March.11.2

DOI: 10.4238/2013.March.11.2

Animal Genetics   Research Article

Isolation and molecular characterization of Leptospira borgpetersenii serovar Hardjo strain Hardjobovis in the urine of naturally infected cattle in Brazil

    R.T. Chideroli1, U.P. Pereira2, D.D. Gonçalves3, A.Y. Nakamura4, A.A. Alfieri5, A.F. Alfieri5 and J.C. Freitas1

Most epidemiologic studies on bovine leptospirosis are based on serological tests that use antibodies against several serotypes, including the serovar Hardjo, which is widespread and considered to be the most adapted to bovine hosts. However, using only serological studies is not sufficient to identify and distinguish species of leptospires. The aim of this study was report the first isolation .. Read More»

Genet. Mol. Res. 15(1): gmr.15018473

DOI: 10.4238/gmr.15018473