C. Zhao
Characterization and development of 56 EST-SSR markers derived from the transcriptome of Odontobutis potamophila
Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic mic.. Read More»
Genet. Mol. Res. 16(2): gmr16029129
DOI: 10.4238/gmr16029129
Correlation of ERCC1 expression in peripheral blood lymphocytes with outcomes of patients with gastric cancer treated with oxaliplatin-based adjuvant chemotherapy
Excision repair cross-complementing gene-1 (ERCC1) is a key regulatory enzyme whose expression patterns in tumor tissues are associated with survival in gastric cancer. The present study aimed to evaluate the effects of ERCC1 expression in peripheral blood lymphocytes (PBLs) on the outcome of patients with gastric cancer treated with oxaliplatin-based adjuvant chemo.. Read More»
Genet. Mol. Res. 14(4): 2015.December.7.3
DOI: 10.4238/2015.December.7.3
Genetic structure and diversity in natural and stocked populations of the mandarin fish (Siniperca chuatsi) in China
The Chinese perch, or mandarin fish (Siniperca chuatsi), is a freshwater fish that is endemic to East Asia. In this study, we investigated the genetic diversity and structure of nine natural mandarin fish populations (from the Yangtze River and Amur River basins) and six hatchery stocks (from central and south China) using microsatellite markers. The results show that the genetic diversity of t.. Read More»
Genet. Mol. Res. 14(2): 2015.May.18.5
DOI: 10.4238/2015.May.18.5
New microsatellite loci for the mandarin fish Siniperca chuatsi and their application in population genetic analysis
The mandarin fish is a popular fresh water food fish in China. Fifty-three polymorphic microsatellite markers were isolated through construction of an enriched library of genomic DNA of Siniperca chuatsi (Percichthyidae). We found 2 to 7 alleles per locus. The observed and expected heterozygosity values varied from 0.059 to 1.000 and from 0.305 to 0.818, respectivel.. Read More»
Genet. Mol. Res. 13(1): 2014.January.22.1
DOI: 10.4238/2014.January.22.1
Increased miR-155-5p expression in dermal mesenchymal stem cells of psoriatic patients: comparing the microRNA expression profile by microarray
Mesenchymal stem cells (MSCs) have pleiotropic immuno-modulatory effects and pro-angiogenic ability, leading to the presumption that MSCs may be involved in the pathogenesis of many inflammatory or autoimmune disorders, including psoriasis. In a previous study, we reported the specific gene expression profile ofdermal MSCs from psoriasis. Inflammation- and angiogene.. Read More»
Genet. Mol. Res. 15(3): gmr.15038631
DOI: 10.4238/gmr.15038631
Characterization and expression analysis of a cyclin B gene from black tiger shrimp (Penaeus monodon)
The open reading frame of black tiger shrimp (Penaeus monodon) cyclin B (Pmcyclin B) was identified, based on cDNA sequence registered in GenBank (accession No. EF015590). The target sequence was 1206 bp, corresponding to 401 amino acids. Two conserved signature sequences of the cyclin B gene family were found in the Pmcyclin B deduced aa sequence. Temporal expression of Pmcyclin B in different.. Read More»
Genet. Mol. Res. 14(4): 2015.October.26.36
DOI: 10.4238/2015.October.26.36
Microsatellite analysis of genetic diversity and genetic structure in five consecutive breeding generations of mandarin fish Siniperca chuatsi (Basilewsky)
In this report, 10 polymorphic microsatellites were applied to assess the genetic diversity and genetic differentiation of 5 consecutive breeding generations of mandarin fish, Siniperca chuatsi (Basilewsky). The results from total number of alleles, average polymorphism information content, and average homozygosity and heterozygosity showed that the genetic diversity of the breeding population .. Read More»
Genet. Mol. Res. 14(1): http://dx.doi.org/2015.March.30.19
DOI: http://dx.doi.org/10.4238/2015.March.30.19
Proteomic analysis of susceptibility in intestinal stromal tumors
We analyzed the susceptibility of intestinal stromal tumors using cell culture and proteomics. Human SGC7901 gastric cells were selected and divided into a blank control group (untransfected SGC7901 cells), a negative control group [SGC7901 cells transfected with negative interference control-small interfering RNA (siRNA)], and a COOH-terminus tensin-like molecule (CTEN)-siRNA-1 group (SGC7901 .. Read More»
Genet. Mol. Res. 14(4): 2015.October.28.16
DOI: 10.4238/2015.October.28.16
Identification and evaluation of endogenous control genes for use in quantitative RT-PCR during wheat (Triticum aestivum L.) grain filling
The use of appropriate reference genes is essential for the generation of accurate and biologically meaningful results from quantitative real-time PCR (qRT-PCR) analysis. However, studies have found that the expression of most commonly used reference genes is not always independent of the tissues, treatments, or developmental stages studied. geNormPlus, NormFinder, and BestKeeper, were applied .. Read More»
Genet. Mol. Res. 14(3): http://dx.doi.org/2015.September.8.15
DOI: http://dx.doi.org/10.4238/2015.September.8.15
Stripe rust resistance and dough quality of new wheat - Dasypyrum villosum translocation lines T1DLâ�?¢1V#3S and T1DSâ�?¢1V#3L and the location of HMW-GS genes
The transfer of agronomically useful genes from wild wheat species into cultivated wheat is one of the most effective approaches to improvement of wheat varieties. To evaluate the transfer of genes from Dasypyrum villosum into Triticum aestivum, wheat quality and disease resistance was evaluated in two new translocation lines, T1DL•1V#3S and T1DS•1V#3L. We examined the levels of strip.. Read More»
Genet. Mol. Res. 14(3): 2015.July.17.16
DOI: 10.4238/2015.July.17.16
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