C.A.F. Melo
Contributions of classical and molecular cytogenetic in meiotic analysis and pollen viability for plant breeding
The analysis of meiotic behavior has been widely used in the study of plants as they provide relevant information about the viability of a species. Meiosis boasts a host of highly conserved events and changes in genes that control these events will give rise to irregularities that can alter the normal course of meiosis and may lead to complete sterility of the plant.. Read More»
Genet. Mol. Res. 16(3): gmr16039582
DOI: 10.4238/gmr16039582
ISSR markers in wild species of Passiflora L. (Passifloraceae) as a tool for taxon selection in ornamental breeding
Inter simple sequence repeat (ISSR) molecular markers were developed and used to investigate interspecific genetic variation in 25 wild species of Passiflora preserved in an active germplasm bank (BAG-Passifloras); intraspecific diversity was also analyzed in P. cincinnata accessions. Of 31 primers tested, 20 identified polymorphic loci with a total of 331 bands, suggesting high polymorphism in.. Read More»
Genet. Mol. Res. 14(4): 2015.December.23.41
DOI: 10.4238/2015.December.23.41
Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora
The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC1) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prepared with the DNA from the paternal geni.. Read More»
Genet. Mol. Res. 14(1): 2015.March.27.4
DOI: 10.4238/2015.March.27.4
Analysis of transferability of microsatellite primers (SSR) in wild Passiflora species and intraspecific genetic diversity in Passiflora alata
The genus Passiflora L. is the most representative of Passifloraceae, with over 500 known species, among which 150-200 originated from Brazil. In addition to the great commercial importance of this genus for the fruit market, many of the species have exotic flowers with a huge diversity of colors and can thereby be exploited as ornamental plants. This study.. Read More»
Genet. Mol. Res. 13(3): 2014.August.7.6
DOI: 10.4238/2014.August.7.6
Multivariate analysis of backcross progeny of Passiflora L. (Passifloraceae) for pre-breeding genotype selection
The Ward-MLM procedure was used to evaluate genetic variation in four backcross progenies and in their parents, hybrid F1 HD13 and donor parent Passiflora sublanceolata. Sixteen quantitative descriptors and five qualitative characteristics of relevance to ornamental flower production were assessed. Using the pseudo-F and pseudo-T² criteria, we identifi.. Read More»
Genet. Mol. Res. 14(4): 2015.November.30.15
DOI: 10.4238/2015.November.30.15
Karyotype variation in cultivars and spontaneous cocoa mutants (Theobroma cacao L.)
Four mutant cocoa accessions with morphological changes and a cultivar sample were karyomorphologically characterized. Slides were prepared by enzymatic digestion of the root meristem and squashed in 45% acetic acid, followed by 2% Giemsa staining. The chromosome number of 2n = 20 was seen in all accessions. The karyotype formula for Cacau Comum and Cacau Rui was 2n = 20m. Submetacentric chromo.. Read More»
Genet. Mol. Res. 12(4): 2013.October.18.5
DOI: 10.4238/2013.October.18.5
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