F.J. Liu

Publications of : F.J. Liu

Plant Genetics Research Article

Molecular identification of variety purity in a cotton hybrid with unknown parentage using DNA-SSR markers

Molecular identification of hybrid purity is difficult in regional trials of cotton varieties and hybrid trials. In particular, the molecular detection of hybrid purity has not yet been reported in the case of unknown parentage. In this study, we screened 5000 pairs of primers and chose 17 pairs of core simple sequence repeat (SSR) primers to determine the F1 purity.. Read More»

Genet. Mol. Res. 16(3): gmr16039799

DOI: 10.4238/gmr16039799

Bioinformatics Research Article

Effects of lipopolysaccharide on the stearoyl-coenzyme A desaturase mRNA level in bovine primary hepatic cells

Q.D. Jiang, H.P. Li, F.J. Liu, X.J. Wang, Y.J. Guo, L.F. Wang, W.F. Lu, H.J. Li, X.P. Li and Y.Y. Wang

This study aimed to compare the effects of lipopolysaccharide (LPS) on stearoyl-coenzyme A desaturase (SCD) gene expression in mouse primary hepatic cells. To obtain sufficient total RNA, primary hepatic cells were plated on 6-cm diameter-type collagen 1-coated dishes (1 x 106 cells per dish). The test was divided into 6 groups with 6 replications per group. The 6 g.. Read More»

Genet. Mol. Res. 13(2): 2014.January.24.12

DOI: 10.4238/2014.January.24.12

Plant Genetics Research Article

Isolation and identification of bovine primary hepatocytes

Q.D. Jiang1, H.P. Li2, F.J. Liu2, X.J. Wang2, Y.J. Guo2, L.F. Wang2, W.F. Lu2, H.J. Li2, X.P. Li1 and G.Y. Yang2

The liver is a unique organ that is endowed with a plethora of specialized functions. Most of its functional traits are controlled by hepatocytes. Primary hepatocytes have been used widely in in vitro models to understand the biological processes occurring in the liver. There are a number of methods used to separate hepatocytes, but the cell activity and purity are much lower in this condition... Read More»

Genet. Mol. Res. 12(4): 2013.October.30.3

DOI: 10.4238/2013.October.30.3