G. Zhao

Molecular analysis of genetic diversity among vine accessions using DNA markers

• D.J. Lei
• G. Zhao
• P. Xie
• Y. Li
• H. Yuan
• M. Zou
• J.G. Niu
• X.F. Ma

Viticulture presents a number of economic and social advantages, such as increasing employment levels and fixing the labor force in rural areas. With the aim of initiating a program of genetic improvement in grapevine from the State University of the state of Rio de Janeiro North Darcy Ribeiro, genetic diversity between 40 genotypes (varieties, rootstock, and specie.. Read More»

Genet. Mol. Res. 16(2): gmr16029586

DOI: 10.4238/gmr16029586

Effects of stathmin 1 silencing by siRNA on sensitivity of esophageal cancer cells Eca-109 to paclitaxel

H.W. Zhu, D. Jiang, Z.Y. Xie, M.H. Zhou, D.Y. Sun and Y.G. Zhao

We investigated the effects of stathmin 1 (STMN1) silencing by small interfering (siRNA) on the sensitivity of esophageal cancer cells Eca-109 to paclitaxel. STMN1 siRNA was transiently transfected into Eca-109 cells. The effects of transfection were detected by quantitative polymerase chain reaction and western blotting. The effects of STMN1 silencing by siRNA on the sensitivity of esophageal .. Read More»

Genet. Mol. Res. 14(4): 2015.December.28.18

DOI: 10.4238/2015.December.28.18

Identifying differential expression genes and single nucleotide variations using RNA-seq in metastatic melanoma

D. Liu1, Z.G. Zhao1, Z.L. Jiao2 and H.J. Li1

Melanoma is a malignant tumor and one of the most frequent metastatic cancers. This study was conducted to identify differential expression genes (DEGs) and single nucleotide variations (SNVs) in metastatic melanoma. We analyzed microarray data of GSE23056 downloaded from the Gene Expression Omnibus, including two normal samples (skinN1 and skinN2) and 2 metastatic melanoma samples (skinT and l.. Read More»

Genet. Mol. Res. 13(4):

Comparative analysis of the liver tissue transcriptomes of Mongolian and Lanzhou fat-tailed sheep

X. Cheng, S.G. Zhao, Y. Yue, Z. Liu, H.W. Li and J.P. Wu

Research on gene regulation has been made possible with the help of RNA sequencing applications such as RNA-Seq technology for high-throughput sequencing platforms. Recent studies have explored the transcriptomes from different tissues of domestic animals using RNA-Seq technology, but little research has been done to study the transcriptomes of breeds of sheep havin.. Read More»

Genet. Mol. Res. 15(2): gmr.15028572

DOI: 10.4238/gmr.15028572

Effects of tail docking on the expression of genes related to lipid metabolism in Lanzhou fat-tailed sheep

Y. Yue, X. Cheng, S.G. Zhao, Z. Liu, L.S. Liu, R. Zhou, J.P. Wu and M.A. Brown

To evaluate stearoyl-CoA desaturase (SCD), hormone-sensitive lipase (HSL), lipoprotein lipase (LPL), and peroxisome proliferator-activated receptor (PPARγ) expression in Lanzhou fat-tailed sheep (with and without docked tails), 18 rams were randomly divided into two equal groups (docked group, LT; control group, LC). These data were also used to increase the u.. Read More»

Genet. Mol. Res. 15(1): gmr.15017323

DOI: 10.4238/gmr.15017323

Molecular cloning and expression analyses of RPS3a gene from mulberry under abiotic stresses and among different mulberry varieties

J. Qian, H. Zhou, M.D. Zhao, H. Wang, F. Li, Y.H. Wang, R.J. Fang, W.G. Zhao and H.J. Kim

A full-length cDNA sequence coding ribosomal protein S3a of mulberry tree, which we designated MmRPS3a (GenBank accession No. KR610331), was cloned based on mulberry expressed sequence tags. Sequence analysis showed that the MmRPS3a is 1089 bp long and contains a 80-bp 5'-UTR (untranslated region) and a 220-bp 3'-UTR. Its open reading frame consists of a 789-bp enco.. Read More»

Genet. Mol. Res. 15(2): gmr.15028129

DOI: 10.4238/gmr.15028129

Production and genetic analysis of resynthesized Brassica napus from a B. rapa landrace from the Qinghai-Tibet Plateau and B. alboglabra

H.D. Liu*, Z.G. Zhao*, D.Z. Du, C.R. Deng and G. Fu

This study aimed to reveal the genetic and epigenetic variations involved in a resynthesized Brassica napus (AACC) generated from a hybridization between a B. rapa (AA) landrace and B. alboglabra (CC). Amplified fragment length polymorphism (AFLP), methylation-sensitive amplified polymorphism, and the cDNA-AFLP technique were performed to detect changes between different generations at the geno.. Read More»

Genet. Mol. Res. 15(1): gmr.15017146

DOI: 10.4238/gmr.15017146

Serum thyroid hormone reference intervals in the apparently healthy individuals of Zhengzhou area of China

P. Wang, Y.J. Gao, J. Cheng, G.L. Kong, Y. Wang, X.Y. Wu, Z.G. Zhao and H.J. Yuan

This study aimed to establish reference intervals for serum thyroid hormones [serum thyroid-stimulating hormone (TSH), triiodothyronine (TT3), thyroxine (TT4), free triiodothyronine (FT3), and free thyroxine (FT4)] in apparently healthy individuals living in Zhengzhou. According to the requirement for laboratory support for the diagnosis and monitoring of thyroid di.. Read More»

Genet. Mol. Res. 13(3): 2014.April.14.15

DOI: 10.4238/2014.April.14.15

Cartilage oligomeric matrix protein and matrix metalloproteinase-3 expression in the serum and joint fluid of a reversible osteoarthritis rabbit model

X.Q. Chu1*, J.J. Wang2*, L.D. Dou1 and G. Zhao1

The main pathological characteristic of osteoarthritis (OA) is cartilage damage. We explored cartilage oligomeric matrix protein (COMP) and matrix metalloproteinase-3 (MMP-3) changes during articular cartilage injury and repair. Rabbits were randomly divided into the following: a blank control group; groups M1, M2, and M3, in which breaking was performed for 2, 4, and 6 weeks, respectively; and.. Read More»

Genet. Mol. Res. 14(4): 2015.November.13.4

DOI: 10.4238/2015.November.13.4

Study on serum proteomic features in patients with and without recurrence or metastasis after surgical resection of esophageal carcinoma

G.B. Zheng, C.F. Gao, X.L. Wang, G. Zhao and D.H. Li

The purpose of this study was to identify specific biomarkers for recurrence or metastasis of esophageal carcinoma in serum of patients subjected to esophagectomy. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) combined with IMAC-Cu2+ ProteinChip array were performed for the serum protein profiling in patients after surg.. Read More»

Genet. Mol. Res. 13(1): 2014.January.21.23

DOI: 10.4238/2014.January.21.23