G.F. Silva

Farmer variety exchange along Amazonian rivers influences the genetic structure of manioc maintained in a regional Brazilian Gene Bank

• S.B. Sousa
• G.F. Silva
• M.C. Dias
• C.R. Clement
• N.R. Sousa

Knowledge of agro-morphological and genetic variability of germplasm diversity is a prerequisite for its use in breeding. The aim of this study was to characterize the genetic diversity of the manioc (Manihot esculenta) Active Germplasm Bank (AGB) at Embrapa Amazônia Ocidental using microsatellite markers. We evaluated 470 accessions collected along five of th.. Read More»

Genet. Mol. Res. 16(3): gmr16039690

DOI: 10.4238/gmr16039690

Operational taxonomic units (OTUs) of endophytic bacteria isolated from banana cultivars in the Amazon

Á. Souza, V.B. Nogueira, J.C. Cruz, N.R. Sousa, A.R.L. Procópio and G.F. Silva

Endophytic microorganisms colonize plants, inhibit the growth of pathogens (by competing for nutrients and/or space), or produce antagonistic substances. Fifty-five endophytic bacteria were isolated from the leaf tissue of the FHIA 18 banana cultivar. Genetic diversity analyses were performed using the enterobacterial repetitive intergenic consensus sequence polymerase chain reaction method and.. Read More»

Genet. Mol. Res. 14(3): 2015.August.19.28

DOI: 10.4238/2015.August.19.28

Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker

G.F. Silva, R.D.V. Paixão, C.B. Queiroz, M.F. Santana, A. Souza, N.R. Sousa, R.E. Hanada and L. Gasparotto

The Enterobacterial repetitive intergenic consensus (ERIC) marker was used to analyze the genetic variability of Mycosphaerella fijiensis, the causative agent of Black Sigatoka disease in banana plants. A total of 123 isolates were used, which were divided into populations based on their original hosts and collection sites in Brazil. A total of 9 loci were .. Read More»

Genet. Mol. Res. 13(3): 2014.September.26.7

DOI: 10.4238/2014.September.26.7

Virulence and genetic diversity among isolates of Mycosphaerella fijiensis in two regions of Brazil

G.F. Silva, V.S. Santos, N.R. Sousa, R.E. Hanada and L. Gasparotto

Black sigatoka, caused by the fungus Mycosphaerella fijiensis (anamorphic stage: Paracercospora fijiensis), was first detected in Brazil in early 1998 in the Benjamin Constant and Tabatinga municipalities in the State of Amazonas, near to where the borders of Brazil, Colombia, and Peru converge. Understanding how cultivars react to the pathogen, and characterizing t.. Read More»

Genet. Mol. Res. 15(2): gmr.15027797

DOI: 10.4238/gmr.15027797

Post-transcriptional silencing of the SGE1 gene induced by a dsRNA hairpin in Fusarium oxysporum f. sp cubense, the causal agent of Panama disease

J.S. Fernandes, P.C.S. Angelo, J.C. Cruz, J.M.M. Santos, N.R. Sousa and G.F. Silva

Fusarium oxysporum f. sp cubense (Foc), the causal agent of Panama disease, is responsible for economic losses in banana crops worldwide. The identification of genes that effectively act on pathogenicity and/or virulence may contribute to the development of different strategies for disease control and the production of resistant plants. The objective of the current .. Read More»

Genet. Mol. Res. 15(2): gmr.15027941

DOI: 10.4238/gmr.15027941

Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum)

A.R. Nascimento, G.F. Silva, G.F. Gualberto and F.L. Almeida

Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change... Read More»

Genet. Mol. Res. 15(4): gmr15049228

DOI: 10.4238/gmr15049228

Distribution of mating-type alleles and M13 PCR markers in the black leaf spot fungus Mycosphaerella fijiensis of bananas in Brazil

C.B. Queiroz E.C. Miranda R.E. Hanada N.R. Sousa L. Gasparotto M.A. Soares G.F. Silva

The fungus Mycosphaerella fijiensis is the causative agent of black sigatoka, which is one of the most destructive diseases of banana plants. Infection with this pathogen results in underdeveloped fruit, with no commercial value. We analyzed the distribution of the M. fijiensis mating-type system and its genetic variability using M13 phage DNA markers. We found a 1:1 distribution of mating-type al.. Read More»

Genet. Mol. Res. 12(1): 2013.February.8.9

DOI: 10.4238/2013.February.8.9

Inter-retrotransposon-amplified polymorphism markers for germplasm characterization in Manihot esculenta (Euphorbiaceae)

A.M. Oliveira-Silva, G.F. Silva, M.C. Dias, C.R. Clement and N.R. Sousa

Manioc, Manihot esculenta, is economically important in many tropical and subtropical countries. The genetic variability of the species has not been fully explored, and new information may help expand its use. Molecular markers based on retrotransposons have good potential for analysis of genetic diversity given their abundance in the genome. Eight long terminal rep.. Read More»

Genet. Mol. Res. 13(2): http://dx.doi.org/2014.May.16.3

DOI: http://dx.doi.org/10.4238/2014.May.16.3

Endophytic bacteria from banana cultivars and their antifungal activity

A. Souza1, J.C. Cruz2, N.R. Sousa2, A.R.L. Procópio1 and G.F. Silva2

Endophytic microorganisms consist of fungi, bacteria, and actinomycetes that play important roles in the process of plant adaptation to the environment. Currently, the natural associations between microorganisms and plant species are being explored for a large number of biotechnological applications. In this study, 122 endophytic bacteria were isolated from 5 cultivars of Musa spp from the stat.. Read More»

Genet. Mol. Res. 13(4):