G.X. Zhang
Polymorphisms of AluI and Hin1I loci of the IGF-1R gene and their genetic effects on growth traits in Bian chickens
Growth traits are important economic traits in broiler chicken production. AluI and Hin1I loci are two restriction sites, which are respectively located in exons 2 and 3 of the IGF-1R gene. These two loci are significantly related to the growth traits in Jinghai Yellow chickens. In this study, a correlation analysis was performed between these two loci and the growt.. Read More»
Genet. Mol. Res. 16(2): gmr16029619
DOI: 10.4238/gmr16029619
Molecular cloning and characterization, and prokaryotic expression of the GnRH1 gene obtained from Jinghai yellow chicken
The gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Recent studies have reported the occurrence of GnRH molecular variants in numerous species. In this study, the GnRH1 gene from Jinghai yellow chicken was cloned by reverse transcriptase-polymerase chain reaction and transformed into BL.. Read More»
Genet. Mol. Res. 14(1): http://dx.doi.org/2015.March.31.14
DOI: http://dx.doi.org/10.4238/2015.March.31.14
Effects of glial cell line-derived neurotrophic factor and leukemia-inhibitory factor on the behavior of two calf testis germline stem cell colony types
Germline stem cells are the only such capable of transmitting genetic information in vivo. The isolation and culture of these cells in vitro provide a unique model to understand sperm differentiation and hence, spermatogenesis and male fertility. In this study, we isolated, purified, and cultured germline stem cells from the testes of newborn calves. Moreover, we in.. Read More»
Genet. Mol. Res. 15(4): gmr15048856
DOI: 10.4238/gmr15048856
In vitro differentiation of sperm from male germline stem cell
In this study, we developed a model of sperm in vitro differentiation to study the mechanism of spermatogenesis. We isolated newborn male germ cells for in vitro differentiation. We found that after 4-5 weeks of culture, sperm-like cells were occasionally observed in cell co-culture and the feed-layer. After 1-2 weeks of culture followed by 4-5 weeks of co-culture, sperm-like cells were observe.. Read More»
Genet. Mol. Res. 14(2): http://dx.doi.org/2015.April.10.5
DOI: http://dx.doi.org/10.4238/2015.April.10.5
Myf5 and MyoG gene SNPs associated with Bian chicken growth trait
The growth trait is important in poultry production. We analyzed the association between single nucleotide polymorphisms (SNPs) in the Myf5 and MyoG gene and Bian chicken growth traits. SNPs in candidate genes of the Bian chickens were detected by the polymerase chain reaction-single strand conformation polymorphism method. Two mutation loci and six genotypes were identified in each candidate g.. Read More»
Genet. Mol. Res. 15(3): gmr.15037043
DOI: 10.4238/gmr.15037043
Single nucleotide polymorphisms associated with growth traits in Jinghai yellow chickens
Body weight is one of the most important economic traits in the poultry industry. In the present study, a custom SNP Beadchip was used to analyze the association between those 15 SNPs and 12 growth traits of Jinghai yellow chickens, and other important genetic parameters were also calculated and analyzed. The results indicated that nine of the 15 SNPs were associate.. Read More»
Genet. Mol. Res. 14(4): 2015.December.8.6
DOI: 10.4238/2015.December.8.6
Identification of 19 loci for reproductive traits in a local Chinese chicken by genome-wide study
Reproductive traits have long been studied and have an important influence on chicken breeding. To identify quantitative trait loci affecting reproductive traits, a genome-wide analysis of a Chinese chicken breed was performed to analyze age at first egg body weight at first egg, first egg weight, egg weight at the age of 300 days, egg weight at the age of 462 days,.. Read More»
Genet. Mol. Res. 16(1): gmr16019431
DOI: 10.4238/gmr16019431
Effect of miRNA-203 on cervical cancer cells and its underlying mechanism
miRNA-203 is involved in the development and progression of various types of cancer. However, its role in cervical cancer remains unclear. The aim of this study was to investigate the effect of miRNA-203 on the proliferation and migration of HeLa cervical cancer cells, as well as survivin expression in these cells. A miRNA-203 primer probe was designed according to a sequence obtained from NCBI.. Read More»
Genet. Mol. Res. 15(3): gmr.15038680
DOI: 10.4238/gmr.15038680
Genome-wide association study of meat quality traits in chicken
Meat quality traits are very important in the poultry industry. To identify single nucleotide polymorphisms (SNPs) and candidate genes affecting meat quality traits, a genome-wide association study was performed using the Illumina chicken 60K SNP beadchip in Jinghai yellow chicken. Four meat quality traits were measured. Two SNPs reached 5% Bonferroni genome-wide significance (P < 1.8E-6) an.. Read More»
Genet. Mol. Res. 14(3): http://dx.doi.org/2015.September.8.6
DOI: http://dx.doi.org/10.4238/2015.September.8.6
Genome-wide association study of growth traits in the Jinghai Yellow chicken
Growth is one of the most economically important traits in the poultry industry. In this study, we identified single-nucleotide polymorphisms (SNPs) and candidate genes associated with growth traits of the Jinghai Yellow chicken. Genome-wide association studies were conducted using the Illumina 60 K SNP Chicken array to genotype 400 Jinghai Yellow chickens. For each.. Read More»
Genet. Mol. Res. 14(4): 2015.November.30.10
DOI: 10.4238/2015.November.30.10
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