H. Yuan
Molecular analysis of genetic diversity among vine accessions using DNA markers
Viticulture presents a number of economic and social advantages, such as increasing employment levels and fixing the labor force in rural areas. With the aim of initiating a program of genetic improvement in grapevine from the State University of the state of Rio de Janeiro North Darcy Ribeiro, genetic diversity between 40 genotypes (varieties, rootstock, and specie.. Read More»
Genet. Mol. Res. 16(2): gmr16029586
DOI: 10.4238/gmr16029586
Transcriptome analysis and de novo annotation of the critically endangered Amur sturgeon (Acipenser schrenckii)
The aim of this study was to provide comprehensive insights into the genetic background of sturgeon by transcriptome study. We performed a de novo assembly of the Amur sturgeon Acipenser schrenckii transcriptome using Illumina Hiseq 2000 sequencing. A total of 148,817 non-redundant unigenes with base length of approximately 121,698,536 bp and ranges from 201 to 26,7.. Read More»
Genet. Mol. Res. 15(2): gmr.15027999
DOI: 10.4238/gmr.15027999
Efficient delivery of connective tissue growth factor shRNA using PAMAM nanoparticles
The aim of this study was to detect the anti-fibrosis activity of connective tissue growth factor (CTGF) small hairpin RNA (shRNA) mediated by polyamidoamine dendrimer nanoparticles in rat myocardial cell lines and myocardium. CTGF shRNAs were constructed from inverted oligonucleotides and a polyamidoamine nanoparticle vector was used to transfer shRNA into H9c2 myo.. Read More»
Genet. Mol. Res. 13(3): 2014.August.28.15
DOI: 10.4238/2014.August.28.15
Polymorphism analysis of the intron one of insulin-like growth factor 2 receptor gene (IGF2R) in FFRC strain common carp (Cyprinus carpio L.) and its relationship with growth performance
The insulin-like growth factor 2 receptor gene (IGF2R) encodes a transmembrane protein receptor and acts to sequester and degrade excess circulating insulin-like growth factor 2, which is critical for normal mammalian growth and development. Thus, IGF2R may serve as a candidate gene underlying growth trait in the common carp. In this study, we isolated the intron one of common carp IGF2R and de.. Read More»
Genet. Mol. Res. 14(1): 2015.January.23.14
DOI: 10.4238/2015.January.23.14
STAT3 gene polymorphisms and susceptibility to non-small cell lung cancer
Signal transducer and activator of transcription protein 3 (STAT3) has been implicated in cancer development and is recognized as a type of oncogene. However, association studies of single nucleotide polymorphisms (SNPs) in the STAT3 gene with cancer risk are rare and not available for lung cancer. We examined whether STAT3 polymorphisms are associated with the risk.. Read More»
Genet. Mol. Res. 10(3): vol10-3gmr1071
DOI: 10.4238/vol10-3gmr1071
Repair of articular cartilage defects by tissue-engineered cartilage constructed with adipose-derived stem cells and acellular cartilaginous matrix in rabbits
After injury, inflammation, or degeneration, articular cartilage has limited self-repair ability. We aimed to explore the feasibility of repair of articular cartilage defects with tissue-engineered cartilage constructed by acellular cartilage matrices (ACMs) seeded with adipose-derived stem cells (ADSCs). The ADSCs were isolated from 3-month-old New Zealand albino r.. Read More»
Genet. Mol. Res. 13(2): http://dx.doi.org/2014.June.18.2
DOI: http://dx.doi.org/10.4238/2014.June.18.2
Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology
Anthropogenic activities have seriously impacted wild resources of the Amur sturgeon, Acipenser schrenckii, and more information on local and regional population genetic structure is required to aid the conservation of this species. In this study, we report the development of 12 novel polymorphic microsatellite loci using next-generation sequencing technology, and the genotyping of 24 individua.. Read More»
Genet. Mol. Res. 14(3): 2015.July.14.16
DOI: 10.4238/2015.July.14.16
Study of optimal scheme of spinal image-guided radiotherapy based on expression of caspase-3 in spinal cord neurons by orthogonal design
The aim of the study was to optimize the biological safety scheme of spinal image-guided radiotherapy (IGRT) by determining the expression of caspase-3 in spinal cord neurons after IGRT. Thirty-six adult male beagles were assigned according to a random number table and subjected to IGRT to the 7th-12th canine thoracic vertebral bodies under a total dose of 80 Gy over 5 weeks. An immunohistochem.. Read More»
Genet. Mol. Res. 14(2): http://dx.doi.org/2015.April.13.1
DOI: http://dx.doi.org/10.4238/2015.April.13.1
Functional characterization of the Ginkgo biloba chalcone synthase gene promoter in transgenic tobacco
The regulative sequence (2273 bp) of the chalcone synthase gene promoter of biloba was cloned by genomic walking. A 2273-bp promoter 5' upstream translation start site of GbCHS was cloned and designated as GbCHSP. pBI121+CHSP:GUS and pBI121-35S:GUS were constructed and transformed into tobacco by LBA4404. We found that GbCHSP could drive transient expression of GUS .. Read More»
Genet. Mol. Res. 13(2): http://dx.doi.org/2014.April.30.6
DOI: http://dx.doi.org/10.4238/2014.April.30.6
Genome-wide multilocus analysis of intraspecific differentiation in Oryza rufipogon Griff. from China and the influence of introgression from O. sativa L.
Twenty-five populations of Oryza rufipogon from China and 144 cultivars of Oryza sativa were selected for this study. Based on the DNA fragment of Ehd1-4 and subspecies-specific sequence-tagged site markers in different chromosomes, intraspecific differentiation in O. rufipogon from China was analyzed. The introgression from O. sativa to O. rufipogon was also analyz.. Read More»
Genet. Mol. Res. 12(4): 2013.December.2.8
DOI: 10.4238/2013.December.2.8
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