I.A. Khan

Assessment of allelic diversity among drought-resistant cotton genotypes using microsatellite markers

• F.S. Awan
• A. Javaid
• F.M. Azhar
• I.A. Khan

Drought, in conjunction with high temperature, is an important environmental constraint to cotton production. Development of cotton varieties with increased tolerance against adverse environmental conditions has been proposed as effective strategy for ensuring reliable yields. In the present study, 30 simple sequence repeat (SSR) primers were used to estimate geneti.. Read More»

Genet. Mol. Res. 16(2): gmr16029664

DOI: 10.4238/gmr16029664

Estimation of genetic distance based on RAPDs between 11 cotton accessions varying in heat tolerance

A.I. Khan, I.A. Khan, F.S. Awan, H.A. Sadaqat and S. Bahadur

The genetic distance of 11 cotton genotypes varying in heat tolerance was studied using RAPD markers. Fifty-three random decamer primers were used for the estimation of genetic distance. Among the 53 RAPD primers, which were custom synthesized by GeneLink Inc., UK, 32 were polymorphic and 21 were monomorphic. The 32 polymorphic primers produced 273 fragments, with a.. Read More»

Genet. Mol. Res. 10(1): vol10-1gmr835

DOI: 10.4238/vol10-1gmr835

Expressed sequence tag-simple sequence repeat-based molecular variance in two Salicornia (Amaranthaceae) populations

Z.L. Xu, Z. Ali, J.X. Yi, X.L. He, D.Y. Zhang, G.H. Yu, A.A. Khan, I.A. Khan and H.X. Ma

Salicornia spp is one of the most salt-tolerant vascular plants and is native to salt marshes and estuaries. We developed expressed sequence tag derived-simple sequence repeat (EST-SSR) markers for estimating genetic diversity and marker-assisted Salicornia breeding. Six polymorphic EST-SSRs of 40 detected 27 alleles, ranging from three to five alleles per locus. Th.. Read More»

Genet. Mol. Res. 10(2):

Elucidation of thermotolerance diversity in cotton (Gossypium hirsutum L.) using physio-molecular approaches

R.M. Rana, S.H. Khan, Z. Ali, A.I. Khan and I.A. Khan

Cotton (Gossypium hirsutum) is an important cash crop, but high temperature during its growing season is one of the major factors that limit its productivity. This problem compels plant breeders to breed for heat tolerance, which can help to overcome this challenge. It is very important to make a comprehensive screening of heat-tolerant genotypes so that only the be.. Read More»

Genet. Mol. Res. 10(2): vol10-2gmr1180

DOI: 10.4238/vol10-2gmr1180

DNA barcoding for species identification in the Palmae family

A. Naeem1, A.A. Khan1, H.M.N. Cheema1, I.A. Khan1 and A. Buerkert2

DNA barcoding is a promising tool for species identification at the molecular level. The barcoding system is well established for species differentiation in animals, while it is less common in plants. We evaluated 2 barcoding regions, maturase K (matK) and ribulose bisphosphate carboxylase (rbcL), to compare species of Palmae according to amplification success, discrimination power, and inter- .. Read More»

Genet. Mol. Res. 13(4): 2014.December.4.29

DOI: 10.4238/2014.December.4.29

Development of a species-specific sequence-characterized amplified region marker for roses

S. Riaz, B. Sadia, F.S. Awan, I.A. Khan, H.A. Sadaqat and I.A. Khan

DNA fingerprints of four rose species, Rosa centifolia, R. Gruss-an-Teplitz, R. bourboniana, and R. damascena, were developed using RAPD-PCR. We identified a unique polymorphic band in R. centifolia. This 762-bp fragment was produced by the random primer GLI-2. The fragment was eluted and directly cloned in a TA cloning vector, pTZ57R/T. Digestion of the plasmid wit.. Read More»

Genet. Mol. Res. 11(1): 2012.February.24.3

DOI: 10.4238/2012.February.24.3

Genetic analysis of drought tolerance with respect to fiber traits in upland cotton

R.A. Nasimi, I.A. Khan, M.A. Iqbal and A.A. Khan

Cotton germplasm was analyzed to investigate its potential for developing water stress tolerance in varieties in the future. Four tolerant (NIAB-78, CIM-482, BH-121, and VH-142) and four susceptible (CIM-446, FH-1000, FH-900, and FH-901) lines were identified of 50 accessions based on their seedling root length. A complete set of diallel crosses among eight selected.. Read More»

Genet. Mol. Res. 15(4): gmr.15048626

DOI: 10.4238/gmr.15048626

Estimation of genetic distance between 10 maize accessions with varying response to different levels of soil moisture

M. Aslam, F.S. Awan, I.A. Khan and A.I. Khan

Ten maize accessions (NC-9, A50-2, M-14, B-42, NC-3, T-7, N-48-1, B-34, USSR, and WFTMS) were studied to estimate the genetic distance on molecular level by random amplified polymorphic DNA. These accessions were selected on the basis of their variable responses against different levels of moisture. Twenty-five primers were used to test genetic diversity, of which 1.. Read More»

Genet. Mol. Res. 8(4): vol8-4gmr606

DOI: 10.4238/vol8-4gmr606

Molecular characterization of wheat germplasm using microsatellite markers

S. Ijaz and I.A. Khan

We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discrimi­nated the accessions and varieties into different clusters; genetic di­versity.. Read More»

Genet. Mol. Res. 8(3):

Identification and authentication of Rosa species through development of species-specific SCAR marker(s)

K.M.I. Bashir, F.S. Awan, I.A. Khan, A.I. Khan and M. Usman

Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the influence of environmental factors, which.. Read More»

Genet. Mol. Res. 13(2): http://dx.doi.org/2014.May.30.8

DOI: http://dx.doi.org/10.4238/2014.May.30.8