J. Bai
Effect of Shenqin biochemical extract on hypoxia-inducible factor-1�± expression in ultraviolet B-irradiated HaCaT cells
Hypoxia-inducible factor-1α (HIF-1α) is considered the main transcriptional regulator of the hypoxia-specific cellular and developmental response. This study was performed to investigate the effect of Shenqin biochemical extract (SQBE) on HIF-1α expression in ultraviolet B (UVB)-irradiated HaCaT cells and the possible action mechanisms of SQBE agai.. Read More»
Genet. Mol. Res. 16(2): gmr16029430
DOI: 10.4238/gmr16029430
Transcriptome analysis of the grass carp (Ctenopharyngodon idella) using 454 pyrosequencing methodology for gene and marker discovery
Total RNA isolated from the brain, muscle, liver, gonad, and intestinal tissues of grass carp was pooled to construct cDNA libraries. Using 454 pyrosequencing, a total of 738,604 high-quality reads were generated from the normalized cDNAs of the pooled individuals. Clustering and assembly of these reads produced a set of 37,086 all-unigene sequences after BLAST. Of these, 24,010 (64.74%) were a.. Read More»
Genet. Mol. Res. 14(4): 2015.December.29.35
DOI: 10.4238/2015.December.29.35
Human kallikrein 5 as a novel prognostic biomarker for triple-negative breast cancer: tissue expression analysis and relationship with disease course
The purposes of this study were to analyze the expression and distribution of human kallikrein 5 (hK5) in triple-negative breast cancer (TNBC) tissues, to establish a standard operating procedure (SOP) for its immunohistochemical assay, and to evaluate the possibility of hK5 being a prognostic biomarker for TNBC. Recombinant hK5 protein and specific antibody were prepared, and the expression an.. Read More»
Genet. Mol. Res. 14(3): 2015.August.14.28
DOI: 10.4238/2015.August.14.28
Cloning and sequence analysis of sucrose phosphate synthase gene from varieties of Pennisetum species
Sucrose phosphate synthase (SPS) is an enzyme used by higher plants for sucrose synthesis. In this study, three primer sets were designed on the basis of known SPS sequences from maize (GenBank: NM_001112224.1) and sugarcane (GenBank: JN584485.1), and five novel SPS genes were identified by RT-PCR from the genomes of Pennisetum spp (the hybrid P. americanum x P. purpureum, P. purpureum Schum., .. Read More»
Genet. Mol. Res. 14(1): http://dx.doi.org/2015.March.31.10
DOI: http://dx.doi.org/10.4238/2015.March.31.10
Leptin and leptin receptor gene polymorphisms are correlated with production performance in the Arctic fox
The polymerase chain reaction-single-strand conformation polymorphism technique was employed to measure mononucleotide diversity in the coding region of the leptin and leptin receptor genes in the Arctic fox. The relationships between specific genetic mutations and reproductive performance in Arctic foxes were determined to improve breeding strategies. We found that a leptin gene polymorphism w.. Read More»
Genet. Mol. Res. 14(2): 2015.May.25.7
DOI: 10.4238/2015.May.25.7
Isolation and characterization of microsatellite markers for Axonopus compressus (Sw.) Beauv. (Poaceae) using 454 sequencing technology
Axonopus compressus (Sw.) Beauv. is a perennial herb widely used as a garden lawn grass. In this study, we used Roche 454 pyrosequencing, combined with the magnetic bead enrichment method FIASCO, to isolate simple sequence repeat markers from the A. compressus genome. A total of 1942 microsatellite loci were identified, with 53,193 raw sequencing reads. One hundred microsatellite loci were sele.. Read More»
Genet. Mol. Res. 14(2): 2015.May.11.2
DOI: 10.4238/2015.May.11.2
Genetic diversity of the Arctic fox using SRAP markers
Sequence-related amplified polymorphism (SRAP) is a recently developed molecular marker technique that is stable, simple, reliable, and achieves moderate to high numbers of codominant markers. This study is the first to apply SRAP markers in a mammal, namely the Arctic fox. In order to investigate the genetic diversity of the Arctic fox and to provide a reference fo.. Read More»
Genet. Mol. Res. 13(4): 2013.December.4.4
DOI: 10.4238/2013.December.4.4
Transcriptional regulation of CD4 gene expression in porcine kidney epithelial cells by virus-like double-stranded RNA and DNA methyltransferase inhibitor
The effects of virus-like double-stranded RNA (dsRNA, PolyI:C) and DNA methyltransferase inhibitor (Aza-CdR) on CD4 gene expression were investigated in a porcine kidney cell line (PK15). We found that expression levels of TLR3 and IFNα were significantly upregulated by PolyI:C, compared to the untreated PK15 cells, which shows that PolyI:C successfully mimics.. Read More»
Genet. Mol. Res. 13(2): http://dx.doi.org/2014.April.29.13
DOI: http://dx.doi.org/10.4238/2014.April.29.13
Novel polymorphic microsatellite markers isolated from the pen shell Atrina pectinata (Mollusca: Bivalvia: Pinnidae)
In this study, we isolated 21 novel polymorphic microsatellite DNA loci from the pen shell Atrina pectinata using magnetic-bead hybridization enrichment. The characteristics of these loci were tested using a population of 30 individuals collected from the Penglai coast, Shandong Province. The number of alleles ranged from 2 to 13, and polymorphism information content (PIC) varied from 0.1730 to.. Read More»
Genet. Mol. Res. 13(4): 2014.December.18.6
DOI: 10.4238/2014.December.18.6
Tissue distribution and early developmental expression patterns of aldolase A, B, and C in grass carp Ctenopharyngodon idellus
Aldolase is a key enzyme involved in glycolysis, gluconeogenesis, and the pentose phosphate pathway. To establish the expression patterns of all three aldolase isozyme genes in different tissues and during early embryogenesis in lower vertebrates, as well as to explore the functional differences between these three isozymes, the grass carp was selected as a model ow.. Read More»
Genet. Mol. Res. 16(3): gmr16039234
DOI: 10.4238/gmr16039234
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