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J. Ha

Publications of : J. Ha
Animal Genetics   Review Article

Effects of cell death-inducing DFF45-like effector B on meat quality traits in Berkshire pigs

Cell death-inducing DFF45-like effector (CIDE) B is a member of the CIDE family of apoptosis-inducing factors. In the present study, we detected a single nucleotide polymorphism (SNP), c.414G>A, which corresponds to the synonymous SNP 414Arg, in CIDE-B in the Berkshire pigs. We also analyzed the relationships between the CIDE-B SNP and various meat quality traits.. Read More»

Genet. Mol. Res. 16(2): gmr16029408

DOI: 10.4238/gmr16029408

Plant Genetics   Research Article

Characterization of SNPs in strawberry cultivars in China

    A.J. Ge J. Han X.D. Li M.Z. Zhao H. Liu Q.H. Dong J.G. Fang

Single nucleotide polymorphisms (SNPs) occur at high frequencies in both plant and animal genomes and can provide broad genome coverage and reliable estimates of genetic relationships. The availability of expressed sequence tag (EST) data has made it feasible to discover SNPs. DNA analysis is crucial in genetic studies not only for strawberry breeding programs but also for characterization of hybr.. Read More»

Genet. Mol. Res. 12(1): 2013.March.7.2

DOI: 10.4238/2013.March.7.2

Plant Genetics   Research Article

Mating system patterns of natural populations of Pinus koraiensis along its post-glacial colonization route in northeastern China

    X. Sui,F.J. Feng,D. Zhao,M. Xing,X.Y. Sun,S.J. Han and M.H. Li

To understand the genetic mechanisms underlying the endangerment of Pinus koraiensis, we studied the mating system of 49 families of this species in 3 natural populations along its post-glacial colonization route across ~1500 km in northeastern China using the chloroplast simple sequence repeat technique. We analyzed 11 polymorphic loci with clear and repeating bands, and we calculated the mult.. Read More»

Genet. Mol. Res. 14(2): http://dx.doi.org/2015.April.27.26

DOI: http://dx.doi.org/10.4238/2015.April.27.26

Microbial Genetics   Research Article

Complete mitochondrial genomes of the Bright Sunbeam Curetis bulis and the Small Copper Lycaena phlaeas (Lepidoptera: Lycaenidae) and their phylogenetic implications

    L.L. Zhang1, J.S. Hao1,2, D.Y. Huang1, X.Y. Sun2, J.J. Hao1, C.M. Peng1 and Q. Yang2

In this study, the complete mitochondrial genomes of Curetis bulis and Lycaena phlaeas were determined and analyzed. The circular genomes are 15,162 bp long for C. bulis and 15,280 bp long for L. phlaeas, with a total A+T content of 82.6 and 83.1%, respectively. Both mitogenomes contain 37 genes, and their gene orders are similar to those of other lepidopterans. All protein-coding genes (PCGs) .. Read More»

Genet. Mol. Res. 12(4): 2013.October.10.9

DOI: 10.4238/2013.October.10.9

Medical Genetics   Research Article

Efficient identification of ornamental peach cultivars using RAPD markers with a manual cultivar identification diagram strategy

    J. Han, W.Y. Wang, X.P. Leng, L. Guo, M.L. Yu, W.B. Jiang and R.J. Ma

One of the most important uses of DNA markers is cultivar identification. However, no DNA fingerprint analysis strategy is available for making DNA markers helpful in practical plant cultivar identification, especially for the identification of a large number of cultivars. We developed a manual cultivar identification diagram strategy for efficient identification of.. Read More»

Genet. Mol. Res. 13(1): 2014.January.8.2

DOI: 10.4238/2014.January.8.2

Human Genetics   Research Article

Decreased expression of humanized Fat-1 in porcine fetal fibroblasts following deletion of PGK-neomycin resistance

    X.J. Han, H. Liang, T. Yun, Y.H. Zhao, M.L. Zhang, L.H. Zhao, R.F. Li and X.L. Li

The neomycin-resistance (neor) gene is widely used as a selectable marker in eukaryotic expression vectors; however, its expression often affects that of target genes. Cre recombinase recognizes LoxP sites, leading to site-specific recombination and deletion of DNA and RNA between two LoxP sites. In the present study, a humanized Fat-1 gene (hFat-1) was generated by DNA Works and used to constr.. Read More»

Genet. Mol. Res. 14(3): http://dx.doi.org/2015.September.28.11

DOI: http://dx.doi.org/10.4238/2015.September.28.11

Animal Genetics   Research Article

Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo

    H.B. Chen, X.C. Zhang, Y.F. Cheng, A. Abdelnasir, S. Tang,N. Kemper, J. Hartung and E.D. Bao

To investigate the mechanism of sudden death as a result of stress-induced damage to heart tissue and myocardial cells and to investigate the cardioprotective role of Hsp70 during heat stress, the distribution and expression of Hsp70 was evaluated in the heart cells of heat-stressed rats in vivo and heat-stressed H9c2 cells in vitro. After exposure to heat stress at 42�?°C for different durations.. Read More»

Genet. Mol. Res. 14(1): 2015.March.20.9

DOI: 10.4238/2015.March.20.9

Cytogenomics   Research Article

Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes

    S. Wei L.S. Zan H.B. Wang G. Cheng M. Du Z. Jiang G.J. Hausman D.C. McFarland M.V. Dodson

Fatty acid binding protein 4 (FABP4) is an important adipocyte gene, with roles in fatty acid transport and fat deposition in animals as well as human metabolic syndrome. However, little is known about the functional regulation of FABP4 at the cellular level in bovine. We designed and selected an effective shRNA (small hairpin RNA) against bovine FABP4, constructed a corresponding adenovirus (A.. Read More»

Genet. Mol. Res. 12(1): 2013.January.4.21

DOI: 10.4238/2013.January.4.21

Plant Genetics   Research Article

Computational identification of miRNAs and their targets in Phaseolus vulgaris

    J. Han, H. Xie, M.L. Kong, Q.P. Sun, R.Z. Li and J.B. Pan

MicroRNAs (miRNAs) are a class of non-coding small RNAs that negatively regulate gene expression at the post-transcriptional level. Although thousands of miRNAs have been identified in plants, limited information is available about miRNAs in Phaseolus vulgaris, despite it being an important food legume worldwide. The high conservation of plant miRNAs enables the ide.. Read More»

Genet. Mol. Res. 13(1): 2014.January.17.16

DOI: 10.4238/2014.January.17.16

Human Genetics   Research Article

A promoter trap vector for knocking out bovine myostatin gene with high targeting efficiency

    L.H. Zhao, Y.H. Zhao, H. Liang, T. Yun, X.J. Han, M.L. Zhang, X. Zhou, D.X. Hou, R.F. Li and X.L. Li

With the development of gene targeting approaches, genomic mutation technologies in livestock animals such as gene trapping, zinc finger nucleases (ZFNs), transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats and their associated systems have been improved. Although ZFNs have been used for gene targeting in many species, the off-target si.. Read More»

Genet. Mol. Res. 14(1): http://dx.doi.org/2015.March.31.5

DOI: http://dx.doi.org/10.4238/2015.March.31.5