L.B. Ma
Isolation and characterization of polymorphic microsatellite markers for the chub mackerel (Scomber japonicus) and cross-species amplification in the blue mackerel (S. australasicus)
In this study, 10 polymorphic microsatellite markers were developed in Scomber japonicus and were examined on 30 individuals collected from the North Pacific. The number of alleles per locus ranged from 4 to 17. The observed and expected heterozygosities per locus ranged from 0.2759 to 0.8621 and from 0.43071 to 0.9177, respectively. The polymorphism information con.. Read More»
Genet. Mol. Res. 16(3): gmr16039712
DOI: 10.4238/gmr16039712
First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers
The mud crab (Scylla paramamosain) is of economic importance for the fisheries and aquaculture industry in China. In this study, we constructed the first genetic linkage map for this species using microsatellite and amplified fragment length polymorphism (AFLP) markers. The map consisted of 65 linkage groups, including 34 triplets and 9 doublets. A total of 212 mole.. Read More»
Genet. Mol. Res. 15(2): gmr.15026929
DOI: 10.4238/gmr.15026929
Characterization and expression analysis of the prophenoloxidase activating factor from the mud crab Scylla paramamosain
Prophenoloxidase activating factors (PPAFs) are a group of clip domain serine proteinases that can convert prophenoloxidase (pro-PO) to the active form of phenoloxidase (PO), causing melanization of pathogens. Here, two full-length PPAF cDNAs from Scylla paramamosain (SpPPAF1 and SpPPAF2) were cloned and characterized. The full-length SpPPAF1 cDNA was 1677 bp in length, including a 5'-untransla.. Read More»
Genet. Mol. Res. 14(3): 2015.August.3.8
DOI: 10.4238/2015.August.3.8
Molecular cloning and gene expression analysis of cystatin C-like proteins in spinyhead croaker Collichthys lucidus
Cystatins are natural tight-binding reversible inhibitors of cysteine proteases. In this study, a cDNA library was constructed from Collichthys lucidus using the SMART technique. A complete cDNA sequence with high identity to the conserved sequence of the cystatin C gene was cloned from the library using EST analysis and rapid amplification of cDNA ends (RACE), then.. Read More»
Genet. Mol. Res. 15(1): gmr.15017417
DOI: 10.4238/gmr.15017417
Characterization, molecular cloning, and expression analysis of Ecsit in the spinyhead croaker, Collichthys lucidus
Evolutionarily conserved signaling intermediate in Toll pathways (Ecsit) is reported to play an essential role in innate immunity, embryogenesis, and assembly or stability of the mitochondrial complex I. In this study, the full-length cDNA of Ecsit was cloned from the spinyhead croaker Collichthys lucidus based on the expressed sequence tags from our cDNA library constructed using the SMART tec.. Read More»
Genet. Mol. Res. 15(1): gmr.15017193
DOI: 10.4238/gmr.15017193
Characteristics of growth traits and their effects on body weight of G1 individuals in the mud crab (Scylla paramamosain)
The mud crab (Scylla paramamosain) is considered a potentially important marine crab species for selective breeding. Here, we first examined sex ratio and differences in 16 growth traits between females and males in a G1 population of S. paramamosain, and we then analyzed the correlation between these growth traits and their effects on body weight .. Read More»
Genet. Mol. Res. 13(3): 2014.August.7.19
DOI: 10.4238/2014.August.7.19
Characterization of hemocyanin from the mud crab Scylla paramamosain and its expression analysis in different tissues, at various stages, and under Vibrio parahaemolyticus infection
Hemocyanin is an important respiratory protein in many arthropod and mollusk species. Here, four cDNAs (SpHc1, SpHc2, SpHc3, and SpHc4), encoding distinct hemocyanin subunits from Scylla paramamosain were cloned using EST analyses and the rapid amplification of cDNA ends. The four full-length cDNA fragments (SpHc1-4) were 2281, 2002, 2184, and 2069 bp, respectively, and they encoded four putati.. Read More»
Genet. Mol. Res. 14(4): 2015
DOI: 10.4238/2015
Development and testing of 13 polymorphic microsatellite markers in Larimichthys polyactis (Sciaenidae) using 5' anchored PCR
Larimichthys polyactis is a commercially important marine fish species in southeast Asia. The population crashed due to overfishing in the 1970s, but has since recovered. We developed 13 novel polymorphic microsatellite markers in L. polyactis using 5' anchored PCR. The characteristics of these loci were estimated by analyzing a sample of 30 individuals. A total of .. Read More»
Genet. Mol. Res. 10(3): vol10-3gmr1464
DOI: 10.4238/vol10-3gmr1464
Optimization of selective breeding through analysis of morphological traits in Chinese sea bass (Lateolabrax maculatus)
Determining correlations between certain traits of economic importance constitutes an essential component of selective activities. In this study, our aim was to provide effective indicators for breeding programs of Lateolabrax maculatus, an important aquaculture species in China. We analyzed correlations between 20 morphometric traits and body weight, using correlation and path analyses. The re.. Read More»
Genet. Mol. Res. 15(3): gmr.15038285
DOI: 10.4238/gmr.15038285
Molecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab Scylla paramamosain
The thioredoxin (Trx) system consists of thioredoxin reductase (TrxR), Trx, and nicotinamide adenine dinucleotide phosphate (NADPH). TrxR is an NADPH-dependent oxidoreductase. Trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. In this study, a cDNA sequence (SpTrx1) showing high identity to the first Tr.. Read More»
Genet. Mol. Res. 13(4): 2014.December.4.19
DOI: 10.4238/2014.December.4.19
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