L.C. Zhang
mRNA and protein expression levels of four candidate genes for ear size in Erhualian and Large White pigs
Porcine ear size is an important characteristic for distinguishing among pig breeds. In a previous genome-wide association study of porcine ear size, LEM domain-containing 3 (LEMD3), methionine sulfoxide reductase B3 (MSRB3), high mobility group AT-hook 2 (HMGA2), and Wnt inhibitory factor 1 (WIF1) were implicated as important candidate genes for ear size. This stud.. Read More»
Genet. Mol. Res. 16(2): gmr16029252
DOI: 10.4238/gmr16029252
Application of retrograde dissection method for isolation of bone marrow cells from rat femurs and tibiae
Currently, there is no practical and efficient method for the isolation of bone marrow cells (BMCs) from rat femurs and tibiae. Here, we attempted to develop a rapid, simple, effective, and non-contaminating method for the isolation of BMCs from rat femurs and tibiae. Rat femurs and tibiae were dissected from the ankle to the hip joint; subsequently, a three-step &l.. Read More»
Genet. Mol. Res. 15(2): gmr.15028178
DOI: 10.4238/gmr.15028178
Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake
Mitogen-activated protein kinase kinase kinase 5 (MAP3K5) is essential for apoptosis, proliferation, differentiation, and immune responses, and is a candidate marker for residual feed intake (RFI) in pig. We cloned the full-length cDNA sequence of porcine MAP3K5 by rapid-amplification of cDNA ends. The 5451-bp gene contains a 5'-untranslated region (UTR) (718 bp), a coding region (3738 bp), and.. Read More»
Genet. Mol. Res. 15(3): gmr.15037998
DOI: 10.4238/gmr.15037998
Association between polymorphisms in insulin-like growth factor-1 and risk of osteoporosis
We conducted a case-control study to assess the relationship between rs35767, rs2288377, and rs5742612 insulin-like growth factor-1 (IGF-1) and osteoporosis risk in a Chinese female population. The genotypes of rs35767, rs2288377, and rs5742612 of IGF-1 were determined by polymerase chain reaction-restriction fragment length polymorphism. Patients with osteoporosis were more likely to have drin.. Read More»
Genet. Mol. Res. 14(3): 2015.July.13.10
DOI: 10.4238/2015.July.13.10
Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3
The signal peptide CUB EGF-like domain-containing protein 3 (SCUBE3) gene is a member of SCUBE gene family and plays important roles in bone cell biology and the determination of limb bone length. In this study, the full-length transcript of porcine SCUBE3 was cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. The full-length sequence of porcine S.. Read More»
Genet. Mol. Res. 15(1): gmr.15017010
DOI: 10.4238/gmr.15017010
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