M.L. Li
Identification of genes related to floral organ development in pak choi by expression profiling
Pak choi is a highly nutritious vegetable that is widely grown in China, Southeast Asia, and other parts of the world. Because it reproduces by seed, it is very important to understand the mechanism of floral organ development. Therefore, using the Chinese cabbage genome as a reference, this study analyzed the expression profiles of shoot apex genes at flower bud di.. Read More»
Genet. Mol. Res. 16(1): gmr16019233
DOI: 10.4238/gmr16019233
Expression of Magnaporthe oryzae genes encoding cysteine-rich proteins secreted during nitrogen starvation and interaction with its host, Oryza sativa
Previous studies have shown that the blast fungus, Magnaporthe oryzae, may experience nitrogen starvation during infection of its plant host (rice,Oryza sativa). Here, we studied the expression of seven genes encoding cysteine-rich proteins with N-terminal signal peptides during nitrogen limitation and throughout the infection process. Some genes were upregulated to a greater extent in weak pat.. Read More»
Genet. Mol. Res. 14(4): 2015.December.16.10
DOI: 10.4238/2015.December.16.10
Identification of differentially expressed genes in a pistillody common wheat mutant using an annealing control primer system
HTS-1 is a new kind of pistillody wheat. All or parts of its stamen are transformed into pistils or pistil-like structures, and it has more seed sets per floret than normal wheat under normal cultivation conditions. To investigate the expression divergence in this mutant, an annealing control primer system was used to identify differentially expressed genes (DEGs) in the young spikelets. As a r.. Read More»
Genet. Mol. Res. 14(2): http://dx.doi.org/2015.April.27.14
DOI: http://dx.doi.org/10.4238/2015.April.27.14
Construction of overexpression vectors of Magnaporthe oryzae genes BAS1 and BAS4 fusion to mCherry and screening of overexpression strains
The aim of this study was to construct overexpression vectors and selecting strains of the Magnaporthe oryzae effectors BAS1 and BAS4. Primer pairs of BAS1, BAS4, and mCherry were designed based on their known nucleotide sequences. The coding sequences of BAS1 and BAS4 were amplified, and the pXY201 plasmid was selected as a template to amplify the mCherry sequence. Fragments of BAS1 and mCherr.. Read More»
Genet. Mol. Res. 14(2): 2015.June.26.17
DOI: 10.4238/2015.June.26.17
Cloning and association analysis of KIT and EDNRB polymorphisms with dominant white coat color in the Chinese raccoon dog (Nyctereutes procyonoides procyonoides)
The Chinese raccoon dog (Nyctereutes procyonoides procyonoides) is one of the most important fur-bearing animal species. The dominant white individual, a coat color variant in farmed Chinese raccoon dog, shows a completely white phenotype over the entire body. The KIT and EDNRB genes have been reported to be associated with the dominant white coat color in some mammalian species. In the present.. Read More»
Genet. Mol. Res. 14(2): 2015.June.12.8
DOI: 10.4238/2015.June.12.8
Novel transcripts of human cytomegalovirus clinical strain found by cDNA library screening
Human cytomegalovirus (HCMV) is a double-stranded DNA virus with the largest genome (~235 kb) of the known human herpes viruses. The coding potential and transcript structures of most HCMV predicted genes have not been identified. New or unknown genes could exist in clinical strains. The SMART (switching mechanism at 5êÃ?Â?Ã?Â? end of RNA template of reverse t.. Read More»
Genet. Mol. Res. 10(2): vol10-2gmr1059
DOI: 10.4238/vol10-2gmr1059
Molecular cloning and characterization of the pseudorabies virus UL31 gene
We amplified a 816-bp sequence of the UL31 gene from the pseudorabies virus (PRV) Becker strain genome. Evidence that this was the UL31 gene was confirmed by cloning and sequencing. The PRV UL31 gene encodes a putative protein of 271-amino acid residues, which was designated the UL31 protein. Bioinformatic analysis indicated that PRV UL31 contains a conserved PHA033.. Read More»
Genet. Mol. Res. 13(1): http://dx.doi.org/2014.March.17.11
DOI: http://dx.doi.org/10.4238/2014.March.17.11
Molecular cloning and characterization of the pseudorabies virus US1 gene
Using polymerase chain reaction, a 1050-bp sequence of the US1 gene was amplified from the pseudorabies virus (PRV) Becker strain genome; identification of the US1 gene was confirmed by further cloning and sequencing. Bioinformatics analysis indicated that the PRV US1 gene encodes a putative polypeptide with 349 amino acids. The encoded protein, designated PICP22, had a conserved Herpes_IE68 domai.. Read More»
Genet. Mol. Res. 12(1): 2013.January.22.7
DOI: 10.4238/2013.January.22.7
Construction and expression of prokaryotic expression vectors fused with genes of Magnaporthe oryzae effector proteins and mCherry
The aim of the current study was to investigate the prokaryotic expression of the Magnaporthe oryzae effector genes BAS1 and BAS4 fused to the fluorescent protein mCherry. Based on previous polymorphic analysis of BAS1 and BAS4 in rice blast strains using PCR, blast strains containing the PCR products of BAS1 and BAS4 were selected for liquid culture for total RNA extraction. For PCR analysis, .. Read More»
Genet. Mol. Res. 14(3): http://dx.doi.org/2015.September.9.21
DOI: http://dx.doi.org/10.4238/2015.September.9.21
A new reduced height gene found in the tetraploid semi-dwarf wheat landrace Aiganfanmai
Aiganfanmai is a dwarf tetraploid wheat landrace (Triticum turgidum var. turgidum) that stably produces the semi-dwarf trait. Plant height varies from 80-105 cm under cultivation. Compared with tall durum wheat (T. turgidum var. durum) variety Langdon, we found it to have short spikes and seeds, besides a semi-dwarf character. We crossed Aiganfanmai with Langdon to .. Read More»
Genet. Mol. Res. 10(4): 2011.October.5.5
DOI: 10.4238/2011.October.5.5
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