M.M. Souza
Contributions of classical and molecular cytogenetic in meiotic analysis and pollen viability for plant breeding
The analysis of meiotic behavior has been widely used in the study of plants as they provide relevant information about the viability of a species. Meiosis boasts a host of highly conserved events and changes in genes that control these events will give rise to irregularities that can alter the normal course of meiosis and may lead to complete sterility of the plant.. Read More»
Genet. Mol. Res. 16(3): gmr16039582
DOI: 10.4238/gmr16039582
ISSR markers in wild species of Passiflora L. (Passifloraceae) as a tool for taxon selection in ornamental breeding
Inter simple sequence repeat (ISSR) molecular markers were developed and used to investigate interspecific genetic variation in 25 wild species of Passiflora preserved in an active germplasm bank (BAG-Passifloras); intraspecific diversity was also analyzed in P. cincinnata accessions. Of 31 primers tested, 20 identified polymorphic loci with a total of 331 bands, suggesting high polymorphism in.. Read More»
Genet. Mol. Res. 14(4): 2015.December.23.41
DOI: 10.4238/2015.December.23.41
Confirmation of cross-fertilization using molecular markers in ornamental passion flower hybrids
Several interspecific Passiflora hybrids are produced in the northern hemisphere for the ornamental plant market. In Brazil, production of passion flower hybrids is limited to the introgression of genes into the main cultivated species, yellow passion fruit, to be used as rootstocks. Confirmation of hybridization in the initial developmental stage is important for b.. Read More»
Genet. Mol. Res. 10(1): vol10-1gmr894
DOI: 10.4238/vol10-1gmr894
Genomic in situ hybridization in plants
Genomic in situ hybridization (GISH), which is a modification of fluorescent in situ hybridization, has been widely used in the study of plants. It has become one of the most important techniques for molecular cytogenetics. GISH is a technique that allows distinguishing the genomes in a cell. With this technique, it is possible to differentiate the genomes in a hybrid; consequently, this tool h.. Read More»
Genet. Mol. Res. 12(3): 2013.August.12.11
DOI: 10.4238/2013.August.12.11
Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora
The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC1) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prepared with the DNA from the paternal geni.. Read More»
Genet. Mol. Res. 14(1): 2015.March.27.4
DOI: 10.4238/2015.March.27.4
Identification of the pattern of heterochromatin distribution in Passiflora species with C-banding
We tested four C-banding protocols to obtain heterochromatic bands in the passion fruit species Passiflora edulis and P. cacaoensis (Passifloraceae). Three of these protocols had been previously described. The three published protocols were not adequate to obtain C-bands in these species. An adapted protocol demonstrated heterochromatin distribution in metaphasic ch.. Read More»
Genet. Mol. Res. 9(3): vol9-3gmr869
DOI: 10.4238/vol9-3gmr869
Analysis of transferability of microsatellite primers (SSR) in wild Passiflora species and intraspecific genetic diversity in Passiflora alata
The genus Passiflora L. is the most representative of Passifloraceae, with over 500 known species, among which 150-200 originated from Brazil. In addition to the great commercial importance of this genus for the fruit market, many of the species have exotic flowers with a huge diversity of colors and can thereby be exploited as ornamental plants. This study.. Read More»
Genet. Mol. Res. 13(3): 2014.August.7.6
DOI: 10.4238/2014.August.7.6
Karyotype variation in cultivars and spontaneous cocoa mutants (Theobroma cacao L.)
Four mutant cocoa accessions with morphological changes and a cultivar sample were karyomorphologically characterized. Slides were prepared by enzymatic digestion of the root meristem and squashed in 45% acetic acid, followed by 2% Giemsa staining. The chromosome number of 2n = 20 was seen in all accessions. The karyotype formula for Cacau Comum and Cacau Rui was 2n = 20m. Submetacentric chromo.. Read More»
Genet. Mol. Res. 12(4): 2013.October.18.5
DOI: 10.4238/2013.October.18.5
Multivariate analysis of morphological characteristics of two species of passion flower with ornamental potential and of hybrids between them
We estimated genetic parameters through multivariate analysis of two species of Passiflora and their hybrids, considered of ornamental potential, based on the morphological characteristics: flower diameter, corona diameter, corona filament size, flower peduncle length, petal length and width, sepal length and width, internode length, stem diameter, leaf length, leaf.. Read More»
Genet. Mol. Res. 10(4): 2011.October.13.3
DOI: 10.4238/2011.October.13.3
Multivariate analysis of backcross progeny of Passiflora L. (Passifloraceae) for pre-breeding genotype selection
The Ward-MLM procedure was used to evaluate genetic variation in four backcross progenies and in their parents, hybrid F1 HD13 and donor parent Passiflora sublanceolata. Sixteen quantitative descriptors and five qualitative characteristics of relevance to ornamental flower production were assessed. Using the pseudo-F and pseudo-T² criteria, we identifi.. Read More»
Genet. Mol. Res. 14(4): 2015.November.30.15
DOI: 10.4238/2015.November.30.15
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