M.P.C. Telles
Discovery and characterization of new microsatellite loci in Dipteryx alata Vogel (Fabaceae) using next-generation sequencing data
The use of next-generation sequencing (NGS) technologies provides a great volume of genome sequence data even for non-model species. The development of microsatellite markers using these data is a relatively quick and easy process. Dipteryx alata Vogel (Fabaceae) is an arboreal species from the Cerrado biome and is considered an important plant genetic resource. Her.. Read More»
Genet. Mol. Res. 16(2): gmr16029639
DOI: 10.4238/gmr16029639
Development of microsatellite markers for the endangered Neotropical tree species Tibouchina papyrus (Melastomataceae)
We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in “cerrado rupestre” from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer design. Leaves from 96 individuals collected .. Read More»
Genet. Mol. Res. 10(1): vol10-1gmr1026
DOI: 10.4238/vol10-1gmr1026
Multi-objective optimization in systematic conservation planning and the representation of genetic variability among populations
Biodiversity crises have led scientists to develop strategies for achieving conservation goals. The underlying principle of these strategies lies in systematic conservation planning (SCP), in which there are at least 2 conflicting objectives, making it a good candidate for multi-objective optimization. Although SCP is typically applied at the species level (or hierarchically higher), it can be .. Read More»
Genet. Mol. Res. 14(2): 2015.June.18.18
DOI: 10.4238/2015.June.18.18
Transferability and characterization of microssatellite loci in Anacardium humile A. St. Hil. (Anacardiaceae)
Microsatellite markers were transferred from the cashew, Anarcadium occidentale, to Anacardium humile (Anacardiaceae), a Neotropical shrub from the Brazilian savanna, that produces an edible nut and pseudo-fruit. We tested 14 microsatellite primers from A. occidentale on A. humile. Polymorphism of each microsatellite locus was analyzed based on 58 individuals from three populations. Twelve loci.. Read More»
Genet. Mol. Res. 12(3): 2013.January.4.24
DOI: 10.4238/2013.January.4.24
Development and characterization of new microsatellites for Eugenia dysenterica DC (Myrtaceae)
Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles per locus (prim.. Read More»
Genet. Mol. Res. 12(3): 2013.February.6.3
DOI: 10.4238/2013.February.6.3
Geographical genetics of Pseudoplatystoma punctifer (Castelnau, 1855) (Siluriformes, Pimelodidae) in the Amazon Basin
Geographical genetics allows the evaluation of evolutionary processes underlying genetic variation within and among local populations and forms the basis for establishing more effective strategies for biodiversity conservation at the population level. In this study, we used explicit spatial analyses to investigate molecular genetic variation (estimated using 7 micro.. Read More»
Genet. Mol. Res. 13(2): http://dx.doi.org/2014.May.9.8
DOI: http://dx.doi.org/10.4238/2014.May.9.8
Genetic characterization of natural populations of the medicinal plant Palicourea coriacea (Rubiaceae) with molecular markers
Palicourea coriacea (Rubiaceae) is a herbaceous, perennial species typical of the Cerrado; it is popularly known as “douradinha”, because of its yellow flowers. It is utilized in popular medicine, mainly for the treatment of renal diseases. We used RAPD markers to evaluate the genetic structure of nine natural populations of P. coriacea, totaling 168 ind.. Read More»
Genet. Mol. Res. 9(2): vol9-2gmr671
DOI: 10.4238/vol9-2gmr671
Genetic diversity of natural populations of Anemopaegma arvense (Bignoniaceae) in the Cerrado of S�?£o Paulo State, Brazil
Catuaba (Anemopaegma arvense), a Bignoniaceae species endemic to Cerrado regions, shows anticancer properties and is widely used as a stimulant in traditional medicine. We evaluated the genetic diversity of seven populations found in the State of São Paulo, using random amplified polymorphic DNA markers. After optimization of the amplification reaction, 10 se.. Read More»
Genet. Mol. Res. 8(1): vol8-1gmr538
DOI: 10.4238/vol8-1gmr538
Exhaustive search for conservation networks of populations representing genetic diversity
Conservation strategies routinely use optimization methods to identify the smallest number of units required to represent a set of features that need to be conserved, including biomes, species, and populations. In this study, we provide R scripts to facilitate exhaustive search for solutions that represent all of the alleles in networks with the smallest possible number of populations. The scri.. Read More»
Genet. Mol. Res. 15(1): gmr.15017525
DOI: 10.4238/gmr.15017525
Development of microsatellite markers for Hancornia speciosa Gomes (Apocynaceae)
Herein, we describe 34 microsatellite loci developed using an enrichment genomic library for the tree species Hancornia speciosa Gomes (Apocynaceae). Thirty-five individuals were genotyped using 34 primers to analyze the polymorphisms at each locus. The number of alleles per locus ranged from 4 to 20. The average number of alleles was 8.11, and the expected heterozygosity ranged from 0.62 to 0... Read More»
Genet. Mol. Res. 14(3): 2015.July.3.2
DOI: 10.4238/2015.July.3.2
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