P. Jiang

Tissue distribution and early developmental expression patterns of aldolase A, B, and C in grass carp Ctenopharyngodon idellus

• J.J. Fan, J.J. Bai
• D.M. Ma, L.Y. Yu
• P. Jiang

Aldolase is a key enzyme involved in glycolysis, gluconeogenesis, and the pentose phosphate pathway. To establish the expression patterns of all three aldolase isozyme genes in different tissues and during early embryogenesis in lower vertebrates, as well as to explore the functional differences between these three isozymes, the grass carp was selected as a model ow.. Read More»

Genet. Mol. Res. 16(3): gmr16039234

DOI: 10.4238/gmr16039234

Transcriptome analysis of the grass carp (Ctenopharyngodon idella) using 454 pyrosequencing methodology for gene and marker discovery

L.Y. Yu, J.J. Bai, J.J. Fan, D.M. Ma, Y.C. Quan and P. Jiang

Total RNA isolated from the brain, muscle, liver, gonad, and intestinal tissues of grass carp was pooled to construct cDNA libraries. Using 454 pyrosequencing, a total of 738,604 high-quality reads were generated from the normalized cDNAs of the pooled individuals. Clustering and assembly of these reads produced a set of 37,086 all-unigene sequences after BLAST. Of these, 24,010 (64.74%) were a.. Read More»

Genet. Mol. Res. 14(4): 2015.December.29.35

DOI: 10.4238/2015.December.29.35

Genetic background of Escherichia coli isolates from peritoneal dialysis patients with peritonitis and uninfected control subjects

Y.F. Li1,, N. Su, S.Y. Chen, W.X. Hu, F.F. Li, Z.P. Jiang and X.Q. Yu

Escherichia coli is the most common cause of Gram-negative peritonitis resulting in peritoneal function deterioration as well as poor clinical outcome in continuous ambulatory peritoneal dialysis (PD) patients. In this study, we analyzed the phylogenetic background and genetic profile of the E. coli isolates and sought to determine the characteristics of specific ba.. Read More»

Genet. Mol. Res. 15(1): gmr.15017341

DOI: 10.4238/gmr.15017341

Isolation and characterization of 32 microsatellite loci for topmouth culter (Culter alburnus Basilewsky)

S.-L. Liu, Z.-M. Gu, Y.-Y. Jia, J.-L. Zhao, W.-P. Jiang, Q. Li and F. Li

The topmouth culter (Culter alburnus) is an economically important freshwater fish, which is widely distributed throughout large rivers, reservoirs, and lake areas of China. We report here the isolation and characterization of 32 new polymorphic microsatellite loci isolated from genomic DNA in this species enriched by (CA)12 and (GA)12 probes. The variability of these microsatellites w.. Read More»

Genet. Mol. Res. 13(3): 2014.September.12.14

DOI: 10.4238/2014.September.12.14

Cloning and functional analysis of the promoter of a maize starch synthase III gene (ZmDULL1)

J.D. Wu, C.P. Jiang, H.S. Zhu, H.Y. Jiang, B.J. Cheng and S.W. Zhu

The ZmDULL1 gene encodes a starch synthase and is a determinant of the structure of endosperm starch in maize (Zea mays L.). However, little is known regarding the regulatory mechanism of the ZmDULL1 gene. In this study, we isolated and characterized the ZmDULL1 promoter (PDULL1), which is the 5' flanking region of ZmDULL1 in maize. Sequence analysis showed that several cisacting elements impor.. Read More»

Genet. Mol. Res. 14(2): 2015.May.22.17

DOI: 10.4238/2015.May.22.17

Effect of retinoic acid on aquaporin 3 expression in keratinocytes

F. Xing W. Liao P. Jiang W. Xu X. Jin

To explore the possible mechanism of the third-generation retinoic acid drugs (isotretinoin, acitretin, adapalene) in inducing skin and mucosa dryness and rhagades; specifically, mechanism by which these drugs influence keratinocyte cell culture models in vitro (HaCaT) and aquaporin channel (AQP3) protein expression was investigated. Isotretinoin, acitretin, and adapalene were applied to human .. Read More»

Genet. Mol. Res. 15(1): gmr.15016951

DOI: 10.4238/gmr.15016951

Polymorphisms of vascular endothelial growth factor on prognosis in hepatocellular carcinoma patients receiving transcatheter arterial chemoembolization treatment

J. Song, L.Z. Wang, X. Li, T.P. Jiang, T.Z. An, M. Xu, X.P. Wu and S. Zhou

We conducted a cohort study to investigate the prognostic significance of vascular endothelial growth factor (VEGF) polymorphisms in hepatocellular carcinoma (HCC) patients after transcatheter arterial chemoembolization (TACE). In total, 156 patients with histologically confirmed HCC within 2 months were collected from January 2007 to January 2008. The genotypes of VEGF-2578C/A, -1154G/A, -634C.. Read More»

Genet. Mol. Res. 13(4):