P.A. Melo Filho
Investigation on possible occurrence of somaclonal effects in zygotic and embryonic cotton structures based on agronomical traits
Zygotic and embryonic cotton structures were used in in vitro procedures to obtain stable plants. Zygotes (24, 48, and 72 h) and immature embryos (10 and 25 days after fertilization) were grown in MS medium supplemented with glucose, indoleacetic acid, kinetin, and activated charcoal. Regenerated seedlings were acclimatized and grown in 10-kg pots for further phenol.. Read More»
Genet. Mol. Res. 16(3): gmr16039711
DOI: 10.4238/gmr16039711
Temporal and tissue expression of genes involved in buds of earliness cotton cultivar
Gene sequences previously identified in Arabidopsis buds were used as references in order to estimate temporal and tissue expression in buds, leaves, stem, and root tissues in cotton plants. Buds were evaluated during 3 phases: 2-8, 10-12, and 14-20 mm. Primers were designed for the ARF6, ATFY, and SEUSS genes for use in semi-quantitative reverse transcription-polymerase chain reaction and quan.. Read More»
Genet. Mol. Res. 14(3): 2015.July.3.14
DOI: 10.4238/2015.July.3.14
Genes expressed in cotton (Gossypium hirsutum) buds isolated with a subtractive library
A subtractive cDNA library from cotton buds was constructed to prospect for differentially expressed genes related to early bud development. A library was constructed and 768 cDNA sequences were obtained, comprising 168 clusters, with 126 contigs and 42 singlets. Both the Gossypium as well as Arabidopsis databases were utilized for the in silico analysis, since some genes identified in cotton h.. Read More»
Genet. Mol. Res. 12(1): 2013.January.16.7
DOI: 10.4238/2013.January.16.7
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