P.P. Wang
Characterization and development of 56 EST-SSR markers derived from the transcriptome of Odontobutis potamophila
Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic mic.. Read More»
Genet. Mol. Res. 16(2): gmr16029129
DOI: 10.4238/gmr16029129
Isolation and identification of new pollen-specific SFB genes in Japanese apricot (Prunus mume)
SFB, a candidate gene for the pollen S gene, has been identified in several species of Prunus (Rosaceae). We isolated 5 new SFB alleles from 6 Japanese apricot (Prunus mume) lines using a specific Prunus SFB primer pair (SFB-C1F and Pm-Vb), which was designed from conserved regions of Prunus SFB. The nucleotide sequences of these SFB genes were submitted to the GenBank database. The 5 new SFB a.. Read More»
Genet. Mol. Res. 12(3): 2013.September.3.5
DOI: 10.4238/2013.September.3.5
Development and characterization of novel EST-SSR markers and their application for genetic diversity analysis of Jerusalem artichoke (Helianthus tuberosus L.)
Jerusalem artichoke (Helianthus tuberosus L.) is a perennial tuberous plant and a traditional inulin-rich crop in Thailand. It has become the most important source of inulin and has great potential for use in chemical and food industries. In this study, expressed sequence tag (EST)-based simple sequence repeat (SSR) markers were developed from 40,362 Jerusalem artic.. Read More»
Genet. Mol. Res. 15(4): gmr15048857
DOI: 10.4238/gmr15048857
Genetic diversity of worldwide Jerusalem artichoke (Helianthus tuberosus) germplasm as revealed by RAPD markers
Jerusalem artichoke (Helianthus tuberosus) is a wild relative of the cultivated sunflower (H. annuus); it is an old tuber crop that has recently received renewed interest. We used RAPD markers to characterize 147 Jerusalem artichoke accessions from nine countries. Thirty RAPD primers were screened; 13 of them detected 357 reproducible RAPD bands, of which 337 were p.. Read More»
Genet. Mol. Res. 10(4): 2011.December.12.4
DOI: 10.4238/2011.December.12.4
High genetic variation among Aschersonia placenta (Clavicipitaceae) isolates from citrus orchards in China
Aschersonia placenta had been recognized as an important fungal pathogen of whiteflies. In recent years, natural occurrence of Aschersonia in whitefly populations was observed in many citrus orchards in the southern regions of China. We analyzed 60 A. placenta isolates obtained from Chinese citrus orchards, using inter-simple sequence repeats to examine the genetic diversity and to determine wh.. Read More»
Genet. Mol. Res. 13(4): 2013.December.4.6
DOI: 10.4238/2013.December.4.6
Comparison of five DNA extraction methods for molecular analysis of Jerusalem artichoke (Helianthus tuberosus)
DNA extraction is an essential step for molecular analysis of an organism, but it is difficult to acquire a sufficient amount of pure DNA from plant tissue with high levels of phenolic compounds, carbohydrates, proteins, and secondary metabolites. Jerusalem artichoke (Helianthus tuberosus) has high levels of such substances. We compared five commonly used methods of.. Read More»
Genet. Mol. Res. 11(1): 2012.March.8.5
DOI: 10.4238/2012.March.8.5
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