R.C. Leite
One-Step RT-qPCR assay for detection and quantification of equine infectious anemia virus in-vitro
Equine infectious anemia virus (EIAV) infection often results in an initial febrile response, followed by recurrent cycles of the disease and finally, a prolonged asymptomatic period. These variations in clinical signs are due to a number of factors, including virus strain, equid species and differences in susceptibility among animals. As a consequence of the close .. Read More»
Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18027
DOI: http://dx.doi.org/10.4238/gmr18027
Genetic variation of bovine leukemia virus (BLV) after replication in cell culture and experimental animals
This article reports the selection of bovine leukemia virus (BLV) variants after continuous passage in cell lines or experimental animals. Two wild BLV strains isolated from 2 naturally infected Holstein dairy cows in Brazil (cow codes: 485 and 141) were used for the experimental infection of 1 sheep and FLK cells, and 1 rabbit and CC81 cells. Viral DNA was isolated.. Read More»
Genet. Mol. Res. 13(1): http://dx.doi.org/2014.January.22.11
DOI: http://dx.doi.org/10.4238/2014.January.22.11
Comparison of three methods of DNA extraction from peripheral blood mononuclear cells and lung fragments of equines
We compared three different protocols for DNA extraction from horse peripheral blood mononuclear cells (PBMC) and lung fragments, determining average final DNA concentration, purity, percentage of PCR amplification using β-actin, and cost. Thirty-four samples from PBMC, and 33 samples from lung fragments were submitted to DNA extraction by three different proto.. Read More»
Genet. Mol. Res. 9(3): vol9-3gmr818
DOI: 10.4238/vol9-3gmr818
Vero cells infected with the Lederle strain of canine distemper virus have increased Fas receptor signaling expression at 15 h post-infection
We evaluated the expression of the Fas receptor gene in Vero cells infected with the Lederle vaccine strain of canine distemper virus using RT-PCR. Vero cells were plated, and after being grown for 24 h in MEM with 5% FBS, 80-90% confluent monolayer cultures were infected with the virus. The cells were harvested at 3, 6, 9, and 15 h post-infection. Uninfected Vero c.. Read More»
Genet. Mol. Res. 10(4): 2011.October.18.3
DOI: 10.4238/2011.October.18.3
Genetic variability and phylogeny of the 5' long terminal repeat from Brazilian bovine leukemia virus
We conducted a phylogenetic analysis of 22 strains of bovine leukemia virus obtained by polymerase chain reaction to amplify a 582-base pair fragment of the transcriptional regulatory region 5' long terminal repeat (LTR). Twenty-two samples of proviral DNA from peripheral blood mononuclear cells containing bovine leukemia virus from naturally infected bovine from 4 distinct geographic regions i.. Read More»
Genet. Mol. Res. 14(4): 2015.November.18.16
DOI: 10.4238/2015.November.18.16
Selection of peptides for serological detection of equine infectious anemia
Equine infectious anemia caused by equine infectious anemia virus is an important disease due to its high severity and incidence in animals. We used a phage display library to isolate peptides that can be considered potential markers for equine infectious anemia diagnosis. We selected peptides using IgG purified from a pool comprised of 20 sera from animals naturally infected with equine infect.. Read More»
Genet. Mol. Res. 11(3): 2012.May.24.2
DOI: 10.4238/2012.May.24.2
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