S. Astolfi-Filho

Prevalence of human papillomavirus, Chlamydia trachomatis, and Trichomonas vaginalis infections in Amazonian women with normal and abnormal cytology

• E. Costa-Lira
• A.H.V.L. Jacinto
• L.M. Silva
• P.F.R. Napoleão
• R.A.A. Barbosa-Filho
• G.J.S. Cruz
• G.J.S. Cruz
• S. Astolfi-Filho
• C.M. Borborema-Santos

Sexually transmitted infections are an important cause of morbidity among sexually active women worldwide, and have been implicated as cofactors in the pathogenesis of cervical cancer. We investigated the prevalence of human papillomavirus (HPV), Chlamydia trachomatis (CT), and Trichomonas vaginalis (TV), and accessed the diversity of HPV in women with normal and ab.. Read More»

Genet. Mol. Res. 16(2): gmr16029626

DOI: 10.4238/gmr16029626

Prevalence of human papillomavirus (HPV) type 16 variants and rare HPV types in the central Amazon region

M.M. Castro, I.P. Farias, C.M. Borborema-Santos, G. Correia and S. Astolfi-Filho

Infection by human papillomavirus (HPV) is one of the primary causes of mortality by cancer in northern Brazil. Sexually active women from Manaus, Amazonas, without cytological alterations and women with pre-malignant and malignant cytological alterations were examined for HPV virus, identified via PCR and sequencing. The target region for this study was part of the.. Read More»

Genet. Mol. Res. 10(1): vol10-1gmr992

DOI: 10.4238/vol10-1gmr992

Method for obtaining high-resolution proteomic analysis from pericarps of guarana

A.L. Souza, P.C.S. Angelo, P.P.O. Nogueira, J.F.C. Gonçalves, A.M. Franco, S. Astolfi-Filho, J.L. López-Lozano and E.V. Andrade

Guarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protei.. Read More»

Genet. Mol. Res. 13(3): 2014.September.29.14

DOI: 10.4238/2014.September.29.14

Identification and isolation of full-length cDNA sequences by sequencing and analysis of expressed sequence tags from guarana (Paullinia cupana)

L.C. Figueirêdo, A.C. Faria-Campos, S. Astolfi-Filho and J.L. Azevedo

The current intense production of biological data, generated by sequencing techniques, has created an ever-growing volume of unanalyzed data. We reevaluated data produced by the guarana (Paullinia cupana) transcriptome sequencing project to identify cDNA clones with complete coding sequences (full-length clones) and complete sequences of genes of biotechnological in.. Read More»

Genet. Mol. Res. 10(2): vol10-2gmr1124

DOI: 10.4238/vol10-2gmr1124

Comparison of bacterial communities in the Solim�µes and Negro River tributaries of the Amazon River based on small subunit rRNA gene sequences

J.C.C. Peixoto, L. Leomil, J.V. Souza, F.B.S. Peixoto and S. Astolfi-Filho

The microbiota of the Amazon River basin has been little studied. We compared the structure of bacterial communities of the Solimões and Negro Rivers, the main Amazon River tributaries, based on analysis of 16S rRNA gene sequences. Water was sampled with a 3-L Van Dorn collection bottle; samples were collected at nine different points/depths totaling 27 L of .. Read More»

Genet. Mol. Res. 10(4):

Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum

I.B. Cordeiro1, D.P. Castro7, P.P.O. Nogueira7, P.C.S. Angelo6, P.A. Nogueira7, J.F.C. Gonçalves8, A.M.R.F. Pereira9, J.S. Garcia2,3, G.H.M.F. Souza2,4, M.A.Z. Arruda5, M.N. Eberlin2, S. Astolfi-Filho1, E.V. Andrade1 and J.L. López-Lozano10

Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry. The total number of spots detected was 1985; the number ranged from 99 to 3.. Read More»

Genet. Mol. Res. 12(4): 2013.October.29.1

DOI: 10.4238/2013.October.29.1

A proteomic approach to guarana seed and pericarp maturation

• A.L. Souza
• J.L. Lopez-Losano
• P.C.S. Angelo
• J.N. Souza-Neto
• I.B. Cordeiro
• S. Astolfi-Filho
• E.V. Andrade

Paullinia cupana Kunth var. sorbilis (Mart.) Ducke, the cultivated guarana plant, is native to the Amazon and has been valued for its medicinal, stimulant and energetic properties for centuries. The seeds are the main commercial product of the plant and the source of high amounts of purine alkaloids (caffeine and theobromine) and polyphenols (flavonoids, catechins, .. Read More»

Genet. Mol. Res. 16(3): gmr16039642

DOI: 10.4238/gmr16039642