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S.M. Santos

Publications of : S.M. Santos
Human Genetics   Research Article

Validation of a reaction volume reduction protocol for analysis of Y chromosome haplotypes targeting DNA databases

The use of Y chromosome haplotypes, important for the detection of sexual crimes in forensics, has gained prominence with the use of databases that incorporate these genetic profiles in their system. Here, we optimized and validated an amplification protocol for Y chromosome profile retrieval in reference samples using lesser materials than those in commercial kits... Read More»

Genet. Mol. Res. 16(2): gmr16029652

DOI: 10.4238/gmr16029652

Human Genetics   Research Article

Distribution of forensic marker allelic frequencies in Pernambuco, Northestern Brazil

    S.M. Santos, C.A. Souza2, K.C.N. Rabelo, P.R.E. Souza,R.R. Moura, T.C. Oliveira, and S. Crovella

Pernambuco is one of the 27 federal units of Brazil, ranking seventh in the number of inhabitants. We examined the allele frequencies of 13 short tandem repeat loci (CFS1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, vWA, and TPOX), the minimum recommended by the Federal Bureau of Investigation and commonly used in forensic genetics laboratories in Brazil, in.. Read More»

Genet. Mol. Res. 14(2): http://dx.doi.org/2015.April.30.2

DOI: http://dx.doi.org/10.4238/2015.April.30.2

Human Genetics   Research Article

Human autosomal DNA and X chromosome STR profiles obtained from Chrysomya albiceps (Diptera: Calliphoridae) larvae used as a biological trace

    T.C. Oliveira1,3, A.B.R. Santos2, K.C.N. Rabelo1,3, C.A. Souza3,S.M. Santos3 and S. Crovella1

The use of insects to answer questions in criminal investigations, as well as a combination of forensic genetic techniques to obtain human DNA from the organisms, especially necrophagous dipterians, have gained ground in recent decades among researchers and professionals in this area. The objective of our study was to evaluate and compare two methods of human DNA ex.. Read More»

Genet. Mol. Res. 15(4): gmr15047622

DOI: 10.4238/gmr15047622

Animal Genetics   Research Article

Detecting multiple DNA human profile from a mosquito blood meal

    K.C.N. Rabêlo, C.M.R. Albuquerque, V.B. Tavares, S.M. Santos, C.A. Souza, T.C. Oliveira, R.R. Moura, L.A.C. Brandão and S. Crovella

Criminal traces commonly found at crime scenes may present mixtures from two or more individuals. The scene of the crime is important for the collection of various types of traces in order to find the perpetrator of the crime. Thus, we propose that hematophagous mosquitoes found at crime scenes can be used to perform genetic testing of human blood and aid in suspect investigation. The aim ofthe.. Read More»

Genet. Mol. Res. 15(3): gmr.15037547

DOI: 10.4238/gmr.15037547

Human Genetics   Research Article

Trace samples of human blood in mosquitoes as a forensic investigation tool

    K.C.N. Rabêlo, C.M.R. Albuquerque, V.B. Tavares, S.M. Santos, C.A. Souza, T.C. Oliveira, N.C.L. Oliveira and S. Crovella

Investigations of any type of crime invariably starts at the crime scene by collecting evidence. Thus, the purpose of this research was to collect and analyze an entomological trace from an environment that is similar to those of indoor crime scenes. Hematophagous mosquitoes were collected from two residential units; saliva of volunteers that were residents in the u.. Read More»

Genet. Mol. Res. 14(4): 2015.November.18.50

DOI: 10.4238/2015.November.18.50