S.W. Yin
Characterization and development of 56 EST-SSR markers derived from the transcriptome of Odontobutis potamophila
Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic mic.. Read More»
Genet. Mol. Res. 16(2): gmr16029129
DOI: 10.4238/gmr16029129
Isolation and characterization of 16 microsatellite loci in marble goby (Oxyeleotris marmoratus)
A microsatellite-enriched genomic library for marble goby (Oxyeleotris marmoratus), a freshwater fish of considerable economic value, was obtained. A total of 16 microsatellite markers were successfully isolated and characterized in a population from the Mekong River in Vietnam. These markers had 2-20 alleles with expected heterozygosity ranging from 0.0370 to 0.8927. Linkage equilibrium was ob.. Read More»
Genet. Mol. Res. 12(2): 2013.January.4.2
DOI: 10.4238/2013.January.4.2
Phylogenetic relationships of twenty Gymnothorax species based on cytochrome b sequence data
To study the phylogenetic relationships of the genus Gymnothorax (moray eels) distributed in South China Sea, polymerase chain reactions were performed, and the amplification products were sequenced by cloning into the PMD18T-vector (TaKaRa). The entire gene sequences encoding cytochrome b (1140 bp) for 16 Gymnothorax (G. flavimarginatus, G. meleagris, G. undulates,.. Read More»
Genet. Mol. Res. 15(2): gmr.15028157
DOI: 10.4238/gmr.15028157
Development and characterization of microsatellite loci in a threatened marine fish, Cheilinus undulatus (humphead wrasse)
Cheilinus undulatus (humphead wrasse) is a marine fish distributed widely throughout the tropical Indo-Pacific. It has been listed as vulnerable in the IUCN Red Data Book and in CITES Appendix II four times. Fifteen microsatellite loci were isolated and characterized for this species. The number of alleles ranged from 3 to 15 per locus, and the observed and expected heterozygosity ranged betwee.. Read More»
Genet. Mol. Res. 12(3): 2013.July.30.2
DOI: 10.4238/2013.July.30.2
Molecular cloning, characterization, and expression of vacuolar-type-H+-ATPase B1 (VHAB1) gene in the gill of Anguilla marmorata
We explored the molecular mechanism of the regulation of vacuolar-type-H+-ATPase B1 (VHAB1) in elvers in the response to salinity. The full-length cDNA of VHAB1 in Anguilla marmorata (designated as AmVHAB1), which was 1741 base pairs (bp) in length, was found to encompass a 1512-bp open reading frame encoding a polypeptide with 503 amino acids (55.9 kDa), an 83-bp 5'-untranslated region, and a .. Read More»
Genet. Mol. Res. 14(3): 2015.July.17.9
DOI: 10.4238/2015.July.17.9
Development and validation of single nucleotide polymorphism markers in Odontobutis potamophila from transcriptomic sequencing
Transcriptome sequencing technology has been applied in the development and discovery of single nucleotide polymorphism (SNP) markers in fish. In this study, a panel of 120 expressed sequence tag (EST)-derived SNPs was selected by several selection filters from the resultant EST library of Odontobutis potamophila using Illumina Sequencing. In total, 37 SNPs from 120 putative SNPs were considere.. Read More»
Genet. Mol. Res. 14(1): 2015.March.20.18
DOI: 10.4238/2015.March.20.18
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