S.Y. Sun

Promoter identification and analysis of key glycosphingolipid biosynthesis-globo series pathway genes in piglets

• W.Y. Qin
• L.N. Gan
• R.W. Xia
• W.H. Dong
• S.Y. Sun
• G.Q. Zhu
• S.L. Wu
• W.B. Bao

Glycosphingolipid biosynthesis-globo series pathway genes (FUT1, FUT2, ST3GAL1, HEXA, HEXB, B3GALNT1, and NAGA) play an important regulatory role in the defense against Escherichia coli F18 in piglets. In this study, we identified the transcription initiation site and promoter of this gene cluster by mined previous RNA-seq results using bioinformatics tools. The FUT.. Read More»

Genet. Mol. Res. 16(2): gmr16029574

DOI: 10.4238/gmr16029574

Cell proliferation and apoptosis in the fetal and neonatal ovary of guinea pigs

S.Y. Sun, W. Zhang, X. Han, R.H. Huang and F.X. Shi

The guinea pig is an excellent animal model for studying reproductive biology of adult humans and most domestic animals. Yet, whether this animal might serve as a good model for embryonic stage investigations and determinations of signals affecting or directing ovary development remains unknown. These questions were addressed by examining morphological evolution and.. Read More»

Genet. Mol. Res. 13(1):

Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility

Z.C. Wu, Y. Liu, W.H. Dong, S.Y. Sun, G.Q. Zhu, S.L. Wu and W.B. Bao

The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recom­binant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, Escher.. Read More»

Genet. Mol. Res. 14(1): 2015.February.6.15

DOI: 10.4238/2015.February.6.15

Agrobacterium-mediated transformation of tomato (Solanum lycopersicum L.) using the expansin 10 (CsEXP10) gene

Y.D. Sun, W.R. Luo, S.Y. Sun and L. Ni

The cucumber expansin 10 (CsEXP10) gene was previously cloned from young cucumber fruits but its role has not been defined. To determine the role of this gene in plant growth and development, a CsEXP10 gene transformation system was established. The open reading frame of the gene was inserted behind the CaMV35S promoter of vector pCAMBIA1301, and the construct was introduced into tomato plants .. Read More»

Genet. Mol. Res. 14(4): 2015

DOI: 10.4238/2015