T.N. Soares

Discovery and characterization of new microsatellite loci in Dipteryx alata Vogel (Fabaceae) using next-generation sequencing data

• R.A. Guimarães
• M.P.C. Telles
• A.M. Antunes
• K.M. Corrêa
• C.V.G. Ribeiro
• A.S.G. Coelho
• T.N. Soares

The use of next-generation sequencing (NGS) technologies provides a great volume of genome sequence data even for non-model species. The development of microsatellite markers using these data is a relatively quick and easy process. Dipteryx alata Vogel (Fabaceae) is an arboreal species from the Cerrado biome and is considered an important plant genetic resource. Her.. Read More»

Genet. Mol. Res. 16(2): gmr16029639

DOI: 10.4238/gmr16029639

Multi-objective optimization in systematic conservation planning and the representation of genetic variability among populations

S. Schlottfeldt, M.E.M.T. Walter, A.C.P. L.F. Carvalho, T.N. Soares, M.P.C. Telles, R.D. Loyola and J.A.F. Diniz-Filho

Biodiversity crises have led scientists to develop strategies for achieving conservation goals. The underlying principle of these strategies lies in systematic conservation planning (SCP), in which there are at least 2 conflicting objectives, making it a good candidate for multi-objective optimization. Although SCP is typically applied at the species level (or hierarchically higher), it can be .. Read More»

Genet. Mol. Res. 14(2): 2015.June.18.18

DOI: 10.4238/2015.June.18.18

Transferability and characterization of microssatellite loci in Anacardium humile A. St. Hil. (Anacardiaceae)

T.N. Soares, L.L. Sant’Ana, L.K. de Oliveira, M.P.C. Telles and R.G. Collevatti

Microsatellite markers were transferred from the cashew, Anarcadium occidentale, to Anacardium humile (Anacardiaceae), a Neotropical shrub from the Brazilian savanna, that produces an edible nut and pseudo-fruit. We tested 14 microsatellite primers from A. occidentale on A. humile. Polymorphism of each microsatellite locus was analyzed based on 58 individuals from three populations. Twelve loci.. Read More»

Genet. Mol. Res. 12(3): 2013.January.4.24

DOI: 10.4238/2013.January.4.24

Development and characterization of new microsatellites for Eugenia dysenterica DC (Myrtaceae)

M.P.C. Telles, J.B. Silva, L.V. Resende, R.P. Vianello, L.J. Chaves, T.N. Soares and R.G. Collevatti

Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles per locus (prim.. Read More»

Genet. Mol. Res. 12(3): 2013.February.6.3

DOI: 10.4238/2013.February.6.3

Genetic characterization of natural populations of the medicinal plant Palicourea coriacea (Rubiaceae) with molecular markers

T.C.S. Barbosa, S.T. Sibov, M.P.C. Telles and T.N. Soares

Palicourea coriacea (Rubiaceae) is a herbaceous, perennial species typical of the Cerrado; it is popularly known as “douradinha”, because of its yellow flowers. It is utilized in popular medicine, mainly for the treatment of renal diseases. We used RAPD markers to evaluate the genetic structure of nine natural populations of P. coriacea, totaling 168 ind.. Read More»

Genet. Mol. Res. 9(2): vol9-2gmr671

DOI: 10.4238/vol9-2gmr671

Geographic shifts in climatically suitable areas and loss of genetic variability in Dipteryx alata (â�?�?Baruâ�? Tree; Fabaceae)

J.A.F. Diniz-Filho, R.G. Collevatti, L.J. Chaves, T.N. Soares, J.C. Nabout, T.F. Rangel, D.B. Melo, J.S. Lima and M.P.C. Telles

Many species are expected to suffer strong shifts in their geographic ranges due to climate changes in the next 50 years, with severe consequences for biodiversity patterns and population structure. We used here an ensemble forecast approach for obtaining species’ range in which multiple species distribution models and climatic models were combined to model lo.. Read More»

Genet. Mol. Res. 11(2): 2012.June.15.11

DOI: 10.4238/2012.June.15.11

Optimizing reproducibility evaluation for random amplified polymorphic DNA markers

J.R. Ramos, M.P.C. Telles, J.A.F. Diniz-Filho, T.N. Soares, D.B. Melo and G. Oliveira

The random amplified polymorphic DNA (RAPD) technique is often criticized because it usually shows low levels of repeatability; thus it can generate spurious bands. These problems can be partially overcome by rigid laboratory protocols and by performing repeatability tests. However, because it is expensive and time-consuming to obtain genetic data twice for all individuals, a few randomly chose.. Read More»

Genet. Mol. Res. 7(4): vol7-4gmr520

DOI: 10.4238/vol7-4gmr520

Transferability of short tandem repeat markers for two wild Canid species inhabiting the Brazilian Cerrado

F.M. Rodrigues, M.P.C. Telles, L.V. Resende, T.N. Soares, J.A.F. Diniz-Filho, A.T.A. Jácomo and L. Silveira

The maned wolf (Chrysocyon brachyurus) and the crab-eating fox (Cerdocyon thous) are two wild-canid species found in the Brazilian Cerrado. We tested cross-amplification and transferability of 29 short tandem repeat primers originally developed for cattle and domestic dogs and cats on 38 individuals of each of these two species, collected in the Emas National Park, .. Read More»

Genet. Mol. Res. 5(4):