W.B. Bao
Promoter identification and analysis of key glycosphingolipid biosynthesis-globo series pathway genes in piglets
Glycosphingolipid biosynthesis-globo series pathway genes (FUT1, FUT2, ST3GAL1, HEXA, HEXB, B3GALNT1, and NAGA) play an important regulatory role in the defense against Escherichia coli F18 in piglets. In this study, we identified the transcription initiation site and promoter of this gene cluster by mined previous RNA-seq results using bioinformatics tools. The FUT.. Read More»
Genet. Mol. Res. 16(2): gmr16029574
DOI: 10.4238/gmr16029574
Age-dependent expression of the BPI gene in Sutai piglets
We compared and analyzed expression of the BPI gene of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 by the real-time PCR method, in order to determine if it is involved in protection against disease caused by ETEC F18. There was a significant difference between 18- and 35-day expression in the jejunum. There were also significant differences between 35-day expressio.. Read More»
Genet. Mol. Res. 12(2): 2012.April.12.1
DOI: 10.4238/2012.April.12.1
TLR4 gene expression in pig populations and its association with resistance to Escherichia coli F18
TLR4 is the main recognition receptor of bacterial lipopolysaccharides, which play an important role in innate and adaptive immunity. We used real-time PCR to analyze the tissue expression profile and differential expression of TLR4 in 4 pig populations (Escherichia coli F18-resistant Sutai, E. coli F18-sensitive Sutai, Large White, Meishan), in order to determine the role that the TLR4 gene pl.. Read More»
Genet. Mol. Res. 12(3): 2013.July.30.1
DOI: 10.4238/2013.July.30.1
Association between polymorphisms in exons 4 and 10 of the BPI gene and immune indices in Sutai pigs
The bactericidal/permeability-increasing protein (BPI) gene has been identified as a candidate gene for disease-resistance breeding. We evaluated whether polymorphisms in exons 4 and 10 of the BPI gene are associated with immune indices [interleukin-2 (IL- 2), IL-4, IL-6, interferon-b (IFN-b), IL-10, and IL-12]. In this study, we identified one mutation (C522T) in the BPI exon 4 site and two mu.. Read More»
Genet. Mol. Res. 14(2): 2015.June.8.2
DOI: 10.4238/2015.June.8.2
Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility
The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recombinant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, Escher.. Read More»
Genet. Mol. Res. 14(1): 2015.February.6.15
DOI: 10.4238/2015.February.6.15
Differential expression of FUT1 and FUT2 in Large White, Meishan, and Sutai porcine breeds
To assess the relationship between the expression of a(1,2)-fucosyltransferase (FUT1 and FUT2) genes and resistance to Escherichia coli F18 in weaned pigs, FUT1 and FUT2 expression levels in Large White, Meishan, and Sutai pigs (with resistance to E. coli F18) were determined using real-time PCR. The results revealed that FUT1 and FUT2 expression levels were higher in the liver, lungs, kidneys,.. Read More»
Genet. Mol. Res. 15(1): gmr.15017613
DOI: 10.4238/gmr.15017613
Nramp1 gene expression in different tissues of Meishan piglets from newborn to weaning
Natural resistance-associated macrophage protein gene 1 (Nramp1) plays an important role in the innate immune response of swine, and is believed to influence disease resistance. In this study, a real-time quantitative polymerase chain reaction technique was used to investigate Nramp1 expression in 12 different tissues in newborn and 7-, 14-, 21-, 28-, and 35-day-old.. Read More»
Genet. Mol. Res. 16(1): gmr16019288
DOI: 10.4238/gmr16019288
Dynamic changes in TAP1 expression levels in newborn to weaning piglets, and its association with Escherichia coli F18 resistance
The transporter associated with antigen processing (TAP) transports peptides from the cytosol into the endoplasmic reticulum for subsequent loading onto the major histocompatibility complex (MHC) class I molecules. This study showed the dynamic changes in the TAP1 expression level in newborn to weaning piglets. Tissue expression profiles revealed that the TAP1 gene .. Read More»
Genet. Mol. Res. 13(2): http://dx.doi.org/2014.May.9.12
DOI: http://dx.doi.org/10.4238/2014.May.9.12
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