W.H. Dong
Promoter identification and analysis of key glycosphingolipid biosynthesis-globo series pathway genes in piglets
Glycosphingolipid biosynthesis-globo series pathway genes (FUT1, FUT2, ST3GAL1, HEXA, HEXB, B3GALNT1, and NAGA) play an important regulatory role in the defense against Escherichia coli F18 in piglets. In this study, we identified the transcription initiation site and promoter of this gene cluster by mined previous RNA-seq results using bioinformatics tools. The FUT.. Read More»
Genet. Mol. Res. 16(2): gmr16029574
DOI: 10.4238/gmr16029574
A simplified universal genomic DNA extraction protocol suitable for PCR
Conventional genomic DNA extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. We developed a simple, time-saving and cost-efficient method for genomic DNA extraction from various types of organisms, using relatively innocuous reagents. The protocol empl.. Read More»
Genet. Mol. Res. 10(1): vol10-1gmr1055
DOI: 10.4238/vol10-1gmr1055
Regulating effects of insertion direction of matrix attachment regions on transgenic expression in stably transformed Chinese hamster ovary cells
We investigated the effects of different directions of insertion of matrix attachment region (MAR) sequences on transgenic expression in stably transformed Chinese hamster ovary (CHO) cells. The MAR sequences were inserted in forward or reverse directions into the expression vectors, and transfected into CHO cells. The expression of the chloramphenicol acetyltransferase (CAT) reporter gene and .. Read More»
Genet. Mol. Res. 14(2): 2015.June.26.12
DOI: 10.4238/2015.June.26.12
Characteristic element of matrix attachment region mediates vector attachment and enhances nerve growth factor expression in Chinese hamster ovary cells
Preliminary studies have suggested that a characteristic element of the matrix attachment region (MAR) in human interferon-β mediates the adhesion of vectors to Chinese hamster ovary (CHO) cells. In this study, we investigated if vector adhesion increased nerve growth factor (NGF) expression in CHO cells. The MAR characteristic element sequence of human interferon-β was inserted into .. Read More»
Genet. Mol. Res. 14(3): 2015.August.7.29
DOI: 10.4238/2015.August.7.29
Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility
The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recombinant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, Escher.. Read More»
Genet. Mol. Res. 14(1): 2015.February.6.15
DOI: 10.4238/2015.February.6.15
Dynamic changes in TAP1 expression levels in newborn to weaning piglets, and its association with Escherichia coli F18 resistance
The transporter associated with antigen processing (TAP) transports peptides from the cytosol into the endoplasmic reticulum for subsequent loading onto the major histocompatibility complex (MHC) class I molecules. This study showed the dynamic changes in the TAP1 expression level in newborn to weaning piglets. Tissue expression profiles revealed that the TAP1 gene .. Read More»
Genet. Mol. Res. 13(2): http://dx.doi.org/2014.May.9.12
DOI: http://dx.doi.org/10.4238/2014.May.9.12
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Citations : 56184
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