X.H. Zhang

A comparative study on genetic characteristics of two new varieties of Pelodiscus sinensis and their hybrid

• H.Q. Zhang
• X.J. Xu
• Z.Y. He
• J.Z. Shao
• X.H. Zhang
• Q.H. Meng
• F.Y. Huang

Chinese soft-shelled turtle Pelodiscus sinensis has been an important aquaculture species in Southeast Asian countries. To breed a new variety of soft-shelled turtle with excellent properties and to evaluate the effect of hybridization of two turtle strains with a highly different trait phenotype, inheritance, microsatellite loci, and transcriptome analysis were stu.. Read More»

Genet. Mol. Res. 16(3): gmr16039148

DOI: 10.4238/gmr16039148

Whole-genome duplications contributed to the expansion of cytochrome b5 genes in Paramecium tetraurelia

K.J. Wu, D. Wang, J. Ding, S.H. Yang and X.H. Zhang

Cytochrome b5 (cyt b5) genes encode ubiquitous electron transport hemoproteins found in animals, plants, fungi, and purple bacteria. However, little is known about their evolutionary history in genomes so far. Here, we conducted an extensive genome-wide survey of cyt b5 genes in 20 representative model species and identified 310 of these genes. Both the absolute number and relative proportion o.. Read More»

Genet. Mol. Res. 12(2): 2013.January.9.1

DOI: 10.4238/2013.January.9.1

Characterization of myostatin gene (MSTN) of Pekin duck and the association of its polymorphism with breast muscle traits

T.S. Xu, L.H. Gu, X.H. Zhang, B.G. Ye, X.L. Liu and S.S. Hou

Myostatin, encoded by the MSTN gene, is a negative regulator of muscle growth, and its expression level in muscle tissue is closely correlated with muscle growth and satellite cell proliferation. To identify the characteristics of the Pekin duck MSTN gene and the relationship between its polymorphism and breast muscle traits in Pekin duck, cDNA cloning and analysis and the expression pattern in.. Read More»

Genet. Mol. Res. 12(3): 2013.February.28.18

DOI: 10.4238/2013.February.28.18

Comprehensive analysis of patched domain-containing genes reveals a unique evolutionary pattern

Y. Zhong, L.J. Gu*, X.G. Sun, S.H. Yang and X.H. Zhang

Patched domain-containing genes are members of a small family originally identified in Drosophila. A common feature of transmembrane patched domain-containing proteins is their function in the transport of sterols, sterol-modified proteins, and lipids. Recently, an expansion phenomenon of patched domain-containing genes was found in Caenorhabditis .. Read More»

Genet. Mol. Res. 13(3): 2014.February.13.11

DOI: 10.4238/2014.February.13.11

MicroRNA-338-3p inhibits glucocorticoid-induced osteoclast formation through RANKL targeting

X.H. Zhang, G.L. Geng, B. Su, C.P. Liang, F. Wang and J.C. Bao

The differentiation deficiencies of osteoclast precursors (pre-OCs) may contribute to osteoporosis. Research on osteoporosis has recently focused on microRNAs (miRNAs) that play crucial roles in pre-OC differentiation. In the current study, we aimed to analyze the expression and function of the glucocorticoid (GC)-associated miRNA-338-3p (miR-338-3p) in osteoclast formation. We found that dexam.. Read More»

Genet. Mol. Res. 15(3): gmr.15037674

DOI: 10.4238/gmr.15037674

Genome-wide identification, phylogenetic relationships, and expression analysis of the carotenoid cleavage oxygenase gene family in pepper

X.H. Zhang, H.Q. Liu, Q.W. Guo, C.F. Zheng, C.S. Li, X.M. Xiang, D.F. Zhao, J. Liu, J. Luo, D.K. Zhao, J.Q. Zheng and H.J. Wan

Carotenoid cleavage oxygenases (CCOs) are a family of dioxygenases, which specifically catalyze the cleavage of conjugated double bonds in carotenoids and apocarotenoids in plants. In this study, genome-wide analysis of CCO genes in pepper plants was performed using bioinformatic methods. At least 11 members of the CCO gene family were identified in the pepper genom.. Read More»

Genet. Mol. Res. 15(4): gmr.15048695

DOI: 10.4238/gmr.15048695

Investigation of AZF microdeletions in patients with Klinefelter syndrome

L.X. Li, H.Y. Dai, X.P. Ding, Y.P. Zhang, X.H. Zhang, H.Y. Ren and Z.Y. Chen

We investigated azoospermia region microdeletions in male infertility patients with Klinefelter syndrome (KFS), as well as the association between azoospermia symptoms in patients with KFS and Y chromosome microdeletion polymorphisms. A total of 111 cases with male infertility confirmed to have KFS (47, XXY) and 94 fertile men were included in this study. Peripheral.. Read More»

Genet. Mol. Res. 14(4): 2015.November.25.2

DOI: 10.4238/2015.November.25.2

Analysis of the dynamic changes in the soft palate and uvula in obstructive sleep apnea-hypopnea using ultrafast magnetic resonance imaging

Y.L. Wang1, J.P. Mcdonald2, Y.H. Liu3, K.F. Pan4, X.H. Zhang5 and R.D. Hu1

Apnea and the respiratory cycle are dynamic processes in obstructive sleep apnea-hypopnea (OSAH), which occur only during sleep. Our study aimed to observe the dynamic changes in the soft palate and the uvula during wakefulness and sleep using ultrafast magnetic resonance imaging (UMRI) to provide reference data for the pathogenesis and treatment of OSAH... Read More»

Genet. Mol. Res. 13(4):

Construction of the mammalian expressing vector pEGFP-N1-P53 and its expression successful in chicken fibroblast cells and blastoderm

Z. Song, Z.H. Li, X.Q. Lei, T.S. Xu, X.H. Zhang, Y.J. Li, G.M. Zhang, S.M. Xi, Y.B. Yang and Z.G. Wei

The enhanced green fluorescent protein (EGFP) pEGFP-N1-P53 eukaryotic expression vector, which contains the human tumor suppressor p53, was constructed and transfected into chicken fibroblast cells and stage-X blastoderm to analyze the transfection efficiency. The complementary DNA of the human p53 gene was cloned by reverse transcription-polymerase chain reaction from human peripheral blood an.. Read More»

Genet. Mol. Res. 14(1): 2015.February.2.16

DOI: 10.4238/2015.February.2.16

Expression of ADAMTS4 and ADAMTS5 in longissimus dorsi muscle related to meat tenderness in Nanyang cattle

X.H. Zhang, Y.X. Qi, X. Gao, J.Y. Li and S.Z. Xu

The ADAMTS4 and ADAMTS5 are secreted proteases, which can cleave aggrecan, brevican and versican to regulate rebuilding of the extracellular matrix. We analyzed the ADAMTS4 and ADAMTS5 gene expression patterns in longissimus dorsi muscle at intervals from 135 days fetal age to 30 months old by qRT-PCR in Nanyang cattle. Expression of ADAMTS4 was significantly higher in 135 and 185-day-old fetus.. Read More»

Genet. Mol. Res. 12(4): 2013.October.18.2

DOI: 10.4238/2013.October.18.2