Y. Wang
A set of novel microsatellite markers developed for an economically important tree, Dracontomelon duperreanum, in China
Dracontomelon duperreanum, the most representative species of the family Anacardiaceae, is an important multipurpose tree in China and Vietnam. However, no genetic diversity studies have been reported on this species. In this study, we identified 11 microsatellite markers for D. duperreanum by using the restriction-site-associated DNA sequencing (RAD-seq) method and.. Read More»
Genet. Mol. Res. 16(2): gmr16029578
DOI: 10.4238/gmr16029578
Isolation and characterization of microsatellite markers for Axonopus compressus (Sw.) Beauv. (Poaceae) using 454 sequencing technology
Axonopus compressus (Sw.) Beauv. is a perennial herb widely used as a garden lawn grass. In this study, we used Roche 454 pyrosequencing, combined with the magnetic bead enrichment method FIASCO, to isolate simple sequence repeat markers from the A. compressus genome. A total of 1942 microsatellite loci were identified, with 53,193 raw sequencing reads. One hundred microsatellite loci were sele.. Read More»
Genet. Mol. Res. 14(2): 2015.May.11.2
DOI: 10.4238/2015.May.11.2
A novel FBN1 heterozygous mutation identified in a Chinese family with autosomal dominant Marfan syndrome
The purpose of this study was to identify the clinical features and mutations in the fibrillin-1 gene (FBN1) in a large Chinese family with autosomal dominant Marfan syndrome (MFS). Seventeen members from a Chinese family of 4 generations were included in the study. All members underwent complete ophthalmic examination. Molecular genetic analysis was performed on all subjects. All exons of FBN1.. Read More»
Genet. Mol. Res. 14(2): http://dx.doi.org/2015.April.27.27
DOI: http://dx.doi.org/10.4238/2015.April.27.27
Implications of microRNA-197 downregulated expression in esophageal cancer with poor prognosis
The aim of this study was to investigate the significance of the microRNA miR-197 expression level in relation to clinicopathological factors and prognoses of esophageal cancer (EC). MicroRNA was extracted using the Taqman® MicroRNA Assay from 46 EC patients at the same tumor node metastasis (TNM) stage, but with different prognoses, who underwent surgery. Parac.. Read More»
Genet. Mol. Res. 13(3): 2014.July.25.12
DOI: 10.4238/2014.July.25.12
Polymorphisms in interleukin-6 and interleukin-10 may be associated with risk of preeclampsia
Preeclampsia is a common condition unique to pregnant women. Previous studies have suggested that several cytokines may contribute to defective placental invasion and endothelial damage in this condition. We investigated the influence of four single nucleotide polymorphisms (SNPs) in the promoters of IL-6 (-572G/C, -597G/A, and -174G/C) and IL-10 (-592A/C) on suscep.. Read More»
Genet. Mol. Res. 16(1): gmr16018588
DOI: 10.4238/gmr16018588
Assessing the association between EFEMP1 rs3791679 polymorphism and risk of glioma in a Chinese Han population
In this study, we assessed the association between the EFEMP1 rs3791679 polymorphism and glioma risk in a Chinese Han population. A total of 94 glioma patients and 206 healthy controls who conformed to the inclusion and exclusion criteria were recruited from Baogang Hospital between March 2012 and October 2014. The EFEMP1 rs3791679 gene polymorphism was assessed using a polymerase chain reactio.. Read More»
Genet. Mol. Res. 15(3): gmr.15038279
DOI: 10.4238/gmr.15038279
Efficacy of Artemisia annua polysaccharides as an adjuvant to hepatitis C vaccination
The traditional Chinese medicine Artemisia annua can prevent and treat hepatitis following an unclear mechanism. The aim of this study was to evaluate the effects of A. annua polysaccharides (AAP) on hepatitis C virus (HCV). A pcDNA3.1/NS3 expression vector was constructed. Ninety female BALB/c mice were randomly divided into six groups: high-dose AAP (1 mg/mL) + HCV/NS3 plasmid; middle-dose AA.. Read More»
Genet. Mol. Res. 14(2): 2015.May.11.29
DOI: 10.4238/2015.May.11.29
Molecular cloning and characterization, and prokaryotic expression of the GnRH1 gene obtained from Jinghai yellow chicken
The gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Recent studies have reported the occurrence of GnRH molecular variants in numerous species. In this study, the GnRH1 gene from Jinghai yellow chicken was cloned by reverse transcriptase-polymerase chain reaction and transformed into BL.. Read More»
Genet. Mol. Res. 14(1): http://dx.doi.org/2015.March.31.14
DOI: http://dx.doi.org/10.4238/2015.March.31.14
Pathology of the lower nasolacrimal duct mucosa at multiple time points after lacrimal retrograde catheterization in rabbit
Lacrimal duct obstruction is a common eye disease, and obstruction of the nasolacrimal duct accounts for over 50% of these cases. Nasolacrimal duct obstruction is usually treated surgically. Lacrimal retrograde catheterization is a novel surgical approach with a high success rate and a relatively high recurrence rate. In this study, we examined the postoperative pathological changes in the lowe.. Read More»
Genet. Mol. Res. 14(3): 2015.July.14.19
DOI: 10.4238/2015.July.14.19
Phylogenetic relationships of Pseudohynobius (Urodela, Hynobiidae) inferred from DNA barcoding analysis
As a proven tool, DNA barcoding can identify species rapidly and unambiguously. In this study, we used mtDNA cyt b, COI, and 16s rRNA sequences of six species of Pseudohynobius, Protohynobius puxiongensis, Liua shihi, Ranodon sibiricus, and Pachyhynobius shangchengensis, to reconstruct the phylogenetic relationships using Bayesian inference and maximum likelihood me.. Read More»
Genet. Mol. Res. 15(2): gmr.15028155
DOI: 10.4238/gmr.15028155
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