Y. Yu
Differential gene expression in the peripheral blood of Chinese Sanhe cattle exposed to severe cold stress
Livestock is an important food resource for the inhabitants of cold regions, such as northern Asia and alpine regions, where agriculture is limited. In these regions, cold stress largely affects livestock production, thereby reducing the productivity and survival of animals. Despite the importance of breeding cold-tolerant animals, few studies have investigated the .. Read More»
Genet. Mol. Res. 16(2): gmr16029593
DOI: 10.4238/gmr16029593
Aberrant promoter methylation of the CD4 gene in peripheral blood cells of mastitic dairy cows
Bovine mastitis is the most common and costly disease of dairy cattle. Cluster of differentiation 4 (CD4) is closely related to the immune response in mastitis. We quantified promoter CpG methylation levels of the CD4 gene in Chinese Holsteins with clinical mastitis (CM) and in healthy controls; these levels were quantitatively detected with bisulfite pyrosequencing assays and confirmed by clon.. Read More»
Genet. Mol. Res. 12(4): 2013.December.4.10
DOI: 10.4238/2013.December.4.10
Expression patterns and promoter activity analysis of UGP2 in pigs.
The function of the UDP-glucose pyrophosphorylase 2 gene (UGP2) in pig is not clear. In the present study, we used RNA isolated from Large White pigs and Chinese indigenous MeiShan pigs to examine the temporal coordination of changes in gene expression within muscle tissues. We cloned both the complete genomic DNA sequence and 2077-bp 5ê�?�?-flanking sequence of po.. Read More»
Genet. Mol. Res. 13(1): http://dx.doi.org/2014.February.28.8
DOI: http://dx.doi.org/10.4238/2014.February.28.8
Development and characterization of polymorphic microsatellite loci in the sea cucumber Holothuria leucospilota
Holothuria leucospilota is a tropical holothurian species that is widely distributed in the tropical and sub-tropical India-Western Pacific Region. Eight polymorphic microsatellite loci were developed from H. leucospilota by using the protocol fast isolation by amplified fragment length polymorphism of sequences containing repeats and tested in 30 individuals from Hainan Island in China. The nu.. Read More»
Genet. Mol. Res. 14(1): 2015.January.26.8
DOI: 10.4238/2015.January.26.8
Molecular characterization, expression patterns, and promoter activity analysis of PGM1 in pigs
The phosphoglucomutase 1 (PGM1) gene was differentially expressed in tissues of Chinese Meishan and Large White pigs. In this study, the promoter region, expression profile, and genetic mutations of the gene were determined. Expression of a 5'-deletion in both C2C12 and PK-15 cells showed that a negative regulatory element was at -1871 to +185 bp and a positive regulatory el.. Read More»
Genet. Mol. Res. 14(1): http://dx.doi.org/2015.March.31.12
DOI: http://dx.doi.org/10.4238/2015.March.31.12
Effects of MMP-1 and MMP-3 gene polymorphisms on gene expression and protein level in lumbar disc herniation
The aim of this study was to identify the possible correlation between polymorphisms in matrix metalloproteinase (MMP)-1 and MMP-3 and their corresponding protein levels in disctissues obtained from patients with lumbar disc herniation (LDH) using biochemical and immunohistochemical analyses. Blood and disc samples were obtained from 100 patients with LDH who underwent a lumbar microdiscectomy... Read More»
Genet. Mol. Res. 15(3): gmr.15038669
DOI: 10.4238/gmr.15038669
Development and characterization of new microsatellite markers of Fenneropenaeus penicillatus
Thirteen new polymorphic microsatellite markers in Fenneropenaeus penicillatus were isolated and characterized. The polymorphism of the thirteen microsatellite markers was tested by 30 individuals from Lianjiang, China. It showed that the number of alleles per locus ranged from 3 to 6 and the Polymorphism Information Content (PIC) was from 0.324 to 0.706. The observed and expected heterozygosit.. Read More»
Genet. Mol. Res. 14(2): 2015.June.18.11
DOI: 10.4238/2015.June.18.11
Isolation and characterization of microsatellite loci in hybrid giant tiger grouper
The hybrid giant tiger grouper is a fish that has considerable commercial value and has become increasingly important for aquaculture in South East Asia since 2008. In order to prevent any reduction in genetic diversity in hybrid grouper as a result of aquaculture, we have identified 21 microsatellite markers that can be used to estimate genetic variation in the fis.. Read More»
Genet. Mol. Res. 14(4): 2015.November.18.35
DOI: 10.4238/2015.November.18.35
Association between the TRAIL single nucleotide polymorphism rs1131580 and type 2 diabetes mellitus in a Han Chinese population
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is expressed in different tissues and cells, including the pancreas and lymphocytes, and it can selectively induce apoptosis in tumor cells but not in most normal cells. TRAIL plays critical roles in type 1 diabetes mellitus, and is involved in type 2 diabetes mellitus (T2DM). We recently discovered the association of nonalco.. Read More»
Genet. Mol. Res. 12(3): 2013.September.10.2
DOI: 10.4238/2013.September.10.2
Association analysis of yield and fiber quality traits in Gossypium barbadense with SSRs and SRAPs
Cotton is an important cash crop. Mining for quantitative trait loci related to yield and fiber quality traits using association analysis has many advantages for cotton research. In this study, 170 simple sequence repeats (SSRs) and 258 sequence-related amplified polymorphisms (SRAPs) were used to analyze the association of 3 yield component traits and 5 fiber quality traits of 55 Gossypium bar.. Read More»
Genet. Mol. Res. 12(3): 2013.September.4.1
DOI: 10.4238/2013.September.4.1
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