Y.C. Wang
Differential gene expression in the peripheral blood of Chinese Sanhe cattle exposed to severe cold stress
Livestock is an important food resource for the inhabitants of cold regions, such as northern Asia and alpine regions, where agriculture is limited. In these regions, cold stress largely affects livestock production, thereby reducing the productivity and survival of animals. Despite the importance of breeding cold-tolerant animals, few studies have investigated the .. Read More»
Genet. Mol. Res. 16(2): gmr16029593
DOI: 10.4238/gmr16029593
Inheritance of cytosine methylation patterns in purebred versus hybrid chicken lines
We used methylation-sensitive amplified polymorphism to examine DNA methylation levels and CCGG patterns in parents and offsprings of 3 groups of adult chickens, purebred White Leghorn (AA), White Plymouth Rock (EE), and crossbred individuals (EA) using 10 primer combinations. We found that about 66% of the cytosines at CCGG sites were not methylated. Fully methylated sites were less frequent t.. Read More»
Genet. Mol. Res. 12(3): 2013.July.30.6
DOI: 10.4238/2013.July.30.6
Orthogonal design in the optimization of a start codon targeted (SCoT) PCR system in Roegneria kamoji Ohwi
Roegneria kamoji Ohwi is an excellent forage grass due to its high feeding value and high resistance to some biotic and abiotic stresses. However, the start codon targeted (SCoT) polymorphism has not been conducted on R. kamoji. In this study, an orthogonal L16 (45) design was employed to investigate the effects of five factors (Mg2+, dNTPs, Taq DNA polymerase, prim.. Read More»
Genet. Mol. Res. 15(4): gmr15048968
DOI: 10.4238/gmr15048968
Identification of single nucleotide polymorphisms in the ASB15 gene and their associations with chicken growth and carcass traits
ASB15 is a member of the ankyrin repeat and suppressor of cytokine signaling box family, and is predominantly expressed in skeletal muscle. In the present study, an F2 resource population of Gushi chickens crossed with Anka broilers was used to investigate the genetic effects of the chicken ASB15 gene. Two single nucleotide polymorphisms (SNPs) (rs315759231 A>G and rs312619270 T>C) were i.. Read More»
Genet. Mol. Res. 14(3): http://dx.doi.org/2015.September.25.5
DOI: http://dx.doi.org/10.4238/2015.September.25.5
Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum
CBL-interacting protein kinases (CIPKs) mediate many plant responses to abiotic stress. However, their functions are poorly understood in halophytes. In this study, we isolated a CIPK gene, NtCIPK2, from the halophyte Nitraria tangutorum. By sequence alignment and the construction of a phylogenetic tree, we found that NtCIPK2 is s.. Read More»
Genet. Mol. Res. 13(3): 2014.July.2.1
DOI: 10.4238/2014.July.2.1
Novel single nucleotide polymorphisms of the bovine methyltransferase 3b gene and their association with meat quality traits in beef cattle
DNA methylation is essential for adipose deposition in mammals. We screened SNPs of the bovine DNA methyltransferase 3b (DNMT3b) gene in Snow Dragon beef, a commercial beef cattle population in China. Nine SNPs were found in the population and three of six novel SNPs were chosen for genotyping and analyzing a possible association with 16 meat quality traits. The frequencies of the alleles and g.. Read More»
Genet. Mol. Res. 11(3): 2012.June.29.1
DOI: 10.4238/2012.June.29.1
Aberrant promoter methylation of the CD4 gene in peripheral blood cells of mastitic dairy cows
Bovine mastitis is the most common and costly disease of dairy cattle. Cluster of differentiation 4 (CD4) is closely related to the immune response in mastitis. We quantified promoter CpG methylation levels of the CD4 gene in Chinese Holsteins with clinical mastitis (CM) and in healthy controls; these levels were quantitatively detected with bisulfite pyrosequencing assays and confirmed by clon.. Read More»
Genet. Mol. Res. 12(4): 2013.December.4.10
DOI: 10.4238/2013.December.4.10
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