Preparation of polyclonal antibody against porcine beta defensin 2 and identification of its distribution in tissues of pig
Authors: Y.Y. Bao, L. Li, H. Zhang, C.Y. Gao, C.B. Xiao and C.L. Li
Porcine β-defensin 2 (pBD2) is an antimicrobial peptide in pigs that plays an important role in the immune system by preventing bacterial invasion. To produce an anti-pBD2 antibody, which is not commercially available, we expressed and purified a soluble, his-tagged version of pBD2 (his-pBD2). Purified pBD2 was injected into New Zealand white rabbits.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.36
- DOI:
- 10.4238/2015.December.28.36
Multiple sequence alignment based on combining genetic algorithm with chaotic sequences
Authors: C. Gao, B. Wang, C.J. Zhou and Q. Zhang
In bioinformatics, sequence alignment is one of the most common problems. Multiple sequence alignment is an NP (nondeterministic polynomial time) problem, which requires further study and exploration. The chaos optimization algorithm is a type of chaos theory, and a procedure for combining the genetic algorithm (GA), which use.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028788
- DOI:
- 10.4238/gmr.15028788
Isolation and characterization of a chalcone isomerase gene promoter from potato cultivars
Authors: M. Chen, W.J. Zhu, X. You, Y.D. Liu, G.M. Kaleri and Q. Yang
Chalcone isomerase (CHI) is a key enzyme involved in anthocyanin metabolism. Previous research on CHI has mainly focused on cDNA cloning and gene expression. In the current study, the 1425-bp potato CHI promoter (PCP) was isolated from four potato cultivars (Heijingang, Zhongshu 7, Désirée, and Favorita) using PCR and DNA sequencing. The PCP.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.37
- DOI:
- 10.4238/2015.December.28.37
Superoxide radical-generating compounds activate a predicted promoter site for paraquat-inducible genes of the Chromobacterium violaceum bacterium in a dose-dependent manner
Authors: J.E. Gabriel, E.P. Guerra-Slompo, E.M. de Souza, F.A.L. de Carvalho, H.M.F. Madeira and A.T.R. de Vasconcelos
The purpose of the present study was to functionally evaluate the influence of superoxide radical-generating compounds on the heterologous induction of a predicted promoter region of open reading frames for paraquat-inducible genes (pqi genes) revealed during genome annotation analyses of the Chromobacterium violaceum bacterium. A 388-bp fragment correspo.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.20
- DOI:
- 10.4238/2015.August.21.20
Daidzein promotes osteoblast proliferation and differentiation in OCT1 cells through stimulating the activation of BMP-2/Smads pathway
Authors: B. Hu, B. Yu, D. Tang, S. Li and Y. Wu
Daidzein, the most widely studied soy phytoestrogen, is not only a potential antiosteoporosis agent owing to its possible osteogenic activity, but also shows anticancer activity. However, the mechanisms through which daidzein affects osteoblast function have not been investigated thoroughly. Here, we show that daidzein stimula.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028792
- DOI:
- 10.4238/gmr.15028792
Identification of genes associated with the increased number of four-seed pods in soybean (Glycine max L.) using transcriptome analysis
Authors: Z.Z. Liu, D. Yao, J. Zhang, Z.L. Li, J. Ma, S.Y. Liu, J. Qu, S.Y. Guan, D.D. Wang, L.D. Pan, D. Wang and P.W. Wang
Seed number per pod is an important component of yield traits in soybean (Glycine max L.). In 2010, we identified a natural mutant with an increased number of four-seed pods from a soybean variety named ‘Jinong 18’ (JN18). Subsequent observations indicated that the trait was stably inherited. To identify and understand the function of genes as.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.39
- DOI:
- 10.4238/2015.December.28.39
Molecular cloning and expression vector construction of bovine TRIM28
Authors: X. Ma, Z.C. Zhai, M.L. Zhang, B.H. Song, Y.R. Zhu, S.B. Yang, X.Q. Dong, L.Y. Su, C.F. Wang, H.X. Ma and W.M. Luan
The bovine TRIM28 gene was amplified from ovary tissue by using RT-PCR. The TRIM28 gene was inserted into the eukaryotic expression vector pIRES2-EGFP and transfected into bovine fetal fibroblasts by using Lipofectamine 3000. TRIM28 mRNA and protein were detected by fluorescence microscope and western blotting. The results sho.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028793
- DOI:
- 10.4238/gmr.15028793
Comparative transcriptome analysis of testes and ovaries for the discovery of novel genes from Amur sturgeon (Acipenser schrenckii)
Authors: S.B. Jin, Y. Zhang, X.L. Dong, Q.K. Xi, D. Song, H.T. Fu and D.J. Sun
Sturgeons (Acipenser schrenckii) are of high evolutionary, economic, and conservation value, and caviar isone of the most valuable animal food products in the world. The Illumina HiSeq2000 sequencing platform was used to construct testicular and ovarian transcriptomes to identify genes involved in reproduction and sex determination in A. schrenckii. A tot.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.40
- DOI:
- 10.4238/2015.December.28.40
Mechanism underlying the bio-effects of an electromagnetic field based on the Huang-Ferrell model
Authors: D.Y. Geng, G. Hu, L. Wang, N. Jia and F.X. Wang
To understand the beneficial and harmful bio-effects of extremely low frequency electromagnetic fields, we studied the MAPK/ERK signaling pathway based on the Huang-Ferrell model. The sensitivity analysis method was used to study the influence of the model parameters on the activity of ERK, and to further investigate the key b.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028796
- DOI:
- 10.4238/gmr.15028796
Genetic characterization of red-colored heartwood genotypes of Chinese fir using simple sequence repeat (SSR) markers
Authors: H.J. Duan, R.Y. Hu, B. Wu, D.X. Chen, K.Y. Huang, J. Dai, Q. Chen, Z.C. Wei, S. Cao, Y.H. Sun and Y. Li
The present study investigated the genetic characterization of red-colored heartwood Chinese fir [Cunninghamia lanceolata (Lamb.) Hook.] in Guangxi using 21 simple sequence repeat (SSR) markers and analyzes of the genetic variation (N = 149) in samples obtained from five sites in Guangxi Province, China. The number of different alleles and the Shannon&rsq.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.2
- DOI:
- 10.4238/2015.December.28.2