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Microbial Genetics   Research Article

Cassava, Manihot esculenta Crantz genetic resources: VI. Anatomy of a diversity center

Authors: N.M.A. Nassar

Central Brasil is one of the four centers of diversity of Manihot species. Of 26 wild Manihot species reported to occur in this region, 20 were collected from two limited areas, each less than 100 km in diameter. These two areas are Goiás Velho and Corumbá de Goiás. Considering the Harlan concept of geogra.. Read More»

Genet. Mol. Res. 2(2):
Microbial Genetics   Research Article

Diallel analysis of production traits among domestic, exotic and mutant germplasms of Lycopersicon

Authors: Guillermo Pratta, Liliana A. Picardi, Roxana Zorzoli

The effects of wild germplasm on tomato fruit shelf life have not yet been completely evaluated. Three different genotypes of Lycopersicon esculentum (a cultivated variety, a homozygote for nor and a homozygote for rin), LA1385 of L. esculentum var. cerasiforme, LA722 of L. pimpinellifolium, and 10 diallel hybrids were assayed.. Read More»

Genet. Mol. Res. 2(2):
Human Genetics   Research Article

Birth of normal kids after microinjection of pronuclear embryos in a transgenic goat (Capra hircus) production program in Brazil

Authors: V.J.F. Freitas, I.A. Serova, L.E. Andreeva, E.S. Lopes J�?ºnior, D.I.A. Teixeira, M.F. Cordeiro, D. Rondina, N.R.O. Paula, I.J. Arruda, J.B. Lima Verde, G. Dvoriantchikov, O. Serov

This pilot project was designed to determine if normal kids could be produced after microinjection in pronuclear embryos and subsequent transfer to recipients in a transgenic goat program in Brazil. Twelve donors of the Saanen breed and 17 recipients of an undefined breed were used. The estrus of both donors and recipients was.. Read More»

Genet. Mol. Res. 2(2):
Microbial Genetics   Research Article

Comparative study of the cuticular hydrocarbon composition of Melipona bicolor Lepeletier, 1836 (Hymenoptera, Meliponini) workers and queens

Authors: F�?¡bio C. Abdalla, Graeme R. Jones, Erik D. Morgan, Carminda da Cruz-Landim

In social insects, cuticular hydrocarbons are involved in species, kin, caste and nestmate recognition. Gas chromatography and mass spectrometry were used to compare the cuticular hydrocarbon composition of workers, males and queens of Melipona bicolor. The cuticular hydrocarbon composition of this species was found to consist.. Read More»

Genet. Mol. Res. 2(2):
Human Genetics   Research Article

Anti-P53 antibodies in Brazilian brain tumor patients

Authors: Renata Fragelli Fonseca, M�?¡rcia Tie Kawamura, Jos�?© Ant�?´nio Oliveira, Anselmo Teixeira, Gilda Alves, Maria da Gl�?³ria da Costa Carvalho

Gliomas of astrocytic origin are the most common primary brain tumors, accounting for over 40 to 50% of all central nervous system tumors. The TP53 tumor suppressor gene is the most frequently mutated gene found in human malignancies. A mutation of this gene can lead to an increased half-life of the resulting protein and loss .. Read More»

Genet. Mol. Res. 2(2):
Human Genetics   Research Article

Evaluation of a method for high yield purification of largely intact mitochondrial DNA from human placentae

Authors: Sergio U. Dani, Alessandra Cristina Gomes-Ruiz, Maria Angela C. Dani

We developed, and quantitatively and qualitatively evaluated an easily reproducible method for high yield purification of mitochondrial DNA (mtDNA) from human placentae by mechanical tissue disruption, differential centrifugation of mitochondria, enzymatic digestion, phenol extraction and ethanol precipitation. Average mtDNA y.. Read More»

Genet. Mol. Res. 2(2):
Microbial Genetics   Research Article

Mining microorganism EST databases in the quest for new proteins

Authors: Alessandra Concei�?§�?£o Faria-Campos, Gustavo Coutinho Cerqueira, Charles Anacleto, Jos�?© Miguel Ortega, Cl�?¡udia M�?¡rcia Benedetto de Carvalho

Microorganisms with large genomes are commonly the subjects of single-round partial sequencing of cDNA, generating expressed sequence tags (ESTs). Usually there is a great distance between gene discovery by EST projects and submission of amino acid sequences to public databases. We analyzed the relationship between available E.. Read More»

Genet. Mol. Res. 2(1):
Microbial Genetics   Research Article

Stage-specific gene expression during Trypanosoma cruzi metacyclogenesis

Authors: Andr�?©a Rodrigues �?vila, Bruno Dallagiovanna, Sueli Fumie Yamada-Ogatta, Viviane Monteiro-G�?³es, St�?ªnio Perdig�?£o Fragoso Marco Aur�?©lio Krieger, Samuel Goldenberg

The process of Trypanosoma cruzi metacyclogenesis involves the transformation of noninfective epimastigotes into metacyclic trypomastigotes, which are the pathogenic form. The analysis of stage-specific genes during T. cruzi metacyclogenesis may provide insight into the mechanisms involved in the regulation of gene expression .. Read More»

Genet. Mol. Res. 2(1):
Microbial Genetics   Research Article

Control of gene expression and genetic manipulation in the Trypanosomatidae

Authors: Santuza M.R. Teixeira, Wanderson D. da Rocha

Mechanisms controlling gene expression in trypanosomatids depend on several layers of regulation, with most regulatory pathways acting at a post-transcriptional level. Consequently, these parasites can follow the rapid changes associated with transitions between the insect vector and the mammalian host, with instant reprogramm.. Read More»

Genet. Mol. Res. 2(1):
Microbial Genetics   Research Article

Sex in fungi: lessons of gene regulation

Authors: Cristina A.J. Souza, Carla C. Silva, Adlane V.-B. Ferreira

Fungi have been very useful for gene regulation studies. Mating implicates in a series of events influenced by many types of environmental input that are interpreted into regulatory pathways, including signal transduction. Although various aspects of mating and signal transduction in the yeast Saccharomyces cerevisiae have lon.. Read More»

Genet. Mol. Res. 2(1):