Evaluation of alternative reporter genes for the yeast two-hybrid system
Authors: Alessandra L. Starling, Kenneth J. Gollob, Elisabete J. Vicente, Gisele M. Andrade-N�³brega, M�´nica B. Rodriguez, J. Miguel Ortega
The yeast two-hybrid system is a powerful tool for screening protein-protein interactions and has also been used for large-scale studies. We evaluated two protein-coding sequences as reporter genes for the yeast two-hybrid system, to determine if it was suitable as an alternative screening strategy. Aspergillus awamori glucoam.. Read More»
- Genet. Mol. Res. 2(1):
Molecular epidemiology of the hepatitis C virus in Brazil
Authors: Solange Busek, Guilherme Oliveira
Hepatitis C virus (HCV) is a major cause of liver disease throughout the world. The genome of this virus consists of approximately 10,000 bp and codes for 10 mature polypeptides. Genome sequence comparison has revealed the existence of six major genotypes and a large number of subtypes. The genotypes can be distinguished by wh.. Read More»
- Genet. Mol. Res. 2(1):
Challenges when transferring technology from Lactococcus laboratory strains to industrial strains
Authors: Eric Johansen
Many genetically modified Lactococcus strains have been constructed in research laboratories around the world. Most of these have originated from laboratory strains and therefore there are several barriers to using them in an industrial setting. Laboratory strains are often plasmid-free and consequently Lac- and Prt-, renderin.. Read More»
- Genet. Mol. Res. 2(1):
Heterologous protein production and delivery systems for Lactococcus lactis
Authors: S�©bastien Nouaille, Luciana A. Ribeiro, Anderson Miyoshi, Daniela Pontes, Yves Le Loir, Sergio Costa Oliveira, Philippe Langella Vasco Azevedo
Lactic acid bacteria (LAB), widely used in the food industry, are present in the intestine of most animals, including humans. The potential use of these bacteria as live vehicles for the production and delivery of heterologous proteins of vaccinal, medical or technological interest has therefore been extensively investigated. .. Read More»
- Genet. Mol. Res. 2(1):
Operon mer: Bacterial resistance to mercury and potential for bioremediation of contaminated environments
Authors: Andr�©a M.A. Nascimento, Edmar Chartone-Souza
Mercury is present in the environment as a result of natural processes and from anthropogenic sources. The amount of mercury mobilized and released into the biosphere has increased since the beginning of the industrial age. Generally, mercury accumulates upwards through aquatic food chains, so that organisms at higher trophic .. Read More»
- Genet. Mol. Res. 2(1):
Escherichia coli as a model system to study DNA repair genes of eukaryotic organisms
Authors: Luiz Augusto-Pinto, Carlos Gustavo Regis da Silva, D�©bora de Oliveira Lopes, Alice Machado-Silva, Carlos Renato Machado
The bacteria Escherichia coli has been widely employed in studies of eukaryotic DNA repair genes. Several eukaryotic genes have been cloned by functional complementation of mutant lineages of E. coli. We examined the similarities and differences among bacterial and eukaryotic DNA repair systems. Based on these data, we examine.. Read More»
- Genet. Mol. Res. 2(1):
Staphylococcus aureus and food poisoning
Authors: Yves Le Loir, Florence Baron, Michel Gautier
Food-borne diseases are of major concern worldwide. To date, around 250 different food-borne diseases have been described, and bacteria are the causative agents of two thirds of food-borne disease outbreaks. Among the predominant bacteria involved in these diseases, Staphylococcus aureus is a leading cause of gastroenteritis r.. Read More»
- Genet. Mol. Res. 2(1):
Efflux as a mechanism of resistance to antimicrobials in Pseudomonas aeruginosa and related bacteria: unanswered questions
Authors: Herbert P. Schweizer
Pseudomonas aeruginosa is an opportunistic human pathogen exhibiting innate resistance to multiple antimicrobial agents. This intrinsic multidrug resistance is caused by synergy between a low-permeability outer membrane and expression of a number of broadly-specific multidrug efflux (Mex) systems, including MexAB-OprM and MexX.. Read More»
- Genet. Mol. Res. 2(1):
GAL4 causes developmental defects and apoptosis when expressed in the developing eye of Drosophila melanogaster
Authors: Jamie M. Kramer, Brian E. Staveley
The UAS/GAL4 ectopic expression system is widely used in Drosophila melanogaster for the overexpression of transgenes. This system operates under the assumption that the yeast transcription factor, GAL4, is inactive in D. melanogaster. Thus, GAL4 can be expressed under the control of D. melanogaster-specific promoters with lit.. Read More»
- Genet. Mol. Res. 2(1):
The influence of brood comb cell size on the reproductive behavior of the ectoparasitic mite Varroa destructor in Africanized honey bee colonies
Authors: Giancarlo A. Piccirillo, D. De Jong
Africanized honey bees (Apis mellifera, Hymenoptera: Apidae) in Brazil are tolerant of infestations with the exotic ectoparasitic mite, Varroa destructor (Mesostigmata: Varroidae), while the European honey bees used in apiculture throughout most of the world are severely affected. Africanized honey bees are normally kept in hi.. Read More»
- Genet. Mol. Res. 2(1):