Female remating, sperm competition and sexual selection in Drosophila
Authors: Shree Ram Singh, Bashisth N. Singh and Hugo F. Hoenigsberg
Female remating is fundamental to evolutionary biology as it determines the pattern of sexual selection and sexual conflict. Remating in females is an important component of Drosophila mating systems because it affects sperm usage patterns and sexual selection. Remating is common in females of many species of Drosophila in bot.. Read More»
- Genet. Mol. Res. 1(3):
Genetic transformation of the lettuce cultivar Grand Rapids (Lactuca sativa L.) by Agrobacterium tumefaciens to improve osmotic stress tolerance (Transforma?§?£o gen?©tica de alface (Lactuca sativa L.), cultivar â??Grand Rapidsâ?, via Agrobacterium tumefaciens, para melhoramento de toler?¢ncia ao estresse h?drico)
Authors: Marcos Pileggi
-.. Read More»
- Genet. Mol. Res. 1(2):
A model system for testing gene vectors using murine tumor cells on the chorioallantoic membrane of the chick embryo
Authors: Sergio U. Dani and Rachel Espindola
We developed a model system for testing gene vectors, based on the growth of murine tumors on the chorioallantoic membrane (CAM) of embryonic chickens. The ability of selected murine cells to grow on the CAM was rated according to the following criteria: i) formation of tumor masses; ii) metastasis formation; iii) reproducibil.. Read More»
- Genet. Mol. Res. 1(2):
Genotoxic evaluation of the organophosphorous pesticide temephos
Authors: Cl?¡udia Alessandra Fortes Aiub, Elis?¢ngela Cristina Alves Coelho, Eduardo Sodr?©, Luis Felipe Ribeiro Pinto and Israel Felzenszwalb
The chemical compound temephos (0,0,0',0'-tetrametyl-0,0'-thiodi-p-phenylene phosphorothioate) is an organophosphorous pesticide that has been used in Brazil since 1967 in control campaigns against the mosquito Aedes aegypti, the vector of dengue and yellow fever. We used single cell gel electrophoresis (SCGE), SOS/umu and Ame.. Read More»
- Genet. Mol. Res. 1(2):
Varroa-tolerant Italian honey bees introduced from Brazil were not more efficient in defending themselves against the mite Varroa destructor than Carniolan bees in Germany
Authors: M.H. Corr?ªa-Marques, D. De Jong, P. Rosenkranz andL.S. Gon?§alves
In Europe and North America honey bees cannot be kept without chemical treatments against Varroa destructor. Nevertheless, in Brazil an isolated population of Italian honey bees has been kept on an island since 1984 without treatment against this mite. The infestation rates in these colonies have decreased over the years. We l.. Read More»
- Genet. Mol. Res. 1(2):
Apomixis and cassava
Authors: N.M.A. Nassar
Apomixis means seed formation without fertilization. In cassava (Manihot esculenta) it is an alternative to reproduction by cuttings, which normally transmits pathogens and leads to an accumulation of viral and bacterial diseases. Apomixis also assures preservation of heterosis and avoids genetic segregation. It occurs in wild.. Read More»
- Genet. Mol. Res. 1(2):
Frequency of the hypervariable DNA loci D18S849, D3S1744, D12S1090 and D1S80 in a mixed ancestry population of Chilean blood donors
Authors: M. Acu?±a, H. Jorquera, L. Cifuentes and L. Armanet
Blood donors (N = 150) at San José Hospital (Santiago, Chile) were typed for one VNTR locus (D1S80) and three STR loci (D18S849, D3S1744, D12S1090). A questionnaire was used to determine the socioeconomic level of the donors, because it is known that some genetic markers (e.g., the ABO and Rh groups) are differentially .. Read More»
- Genet. Mol. Res. 1(2):
Cell nucleus activity during post-embryonic development of Apis mellifera L. (Hymenoptera:Apidae). Intranuclear acid phosphatase
Authors: Carminda da Cruz Landim, Rejane Daniele Reginato, Regina Lucia Morelli Silva de Moraes and Vagner Melo Cavalcante
We report nuclear acid phosphatase activity in the somatic (intra-ovariolar and stromatic) and germ cells of differentiating honey bee worker ovaries, as well as in the midgut cells of metamorphosing bees. There was heterogeneity in the intensity and distribution of electron dense deposits of lead phosphate, indicative of acid.. Read More»
- Genet. Mol. Res. 1(2):
A technique to obtain fibroblast cells from skin biopsies of living bats (Chiroptera) for cytogenetic studies
Authors: Ricardo Moratelli, Cl?¡udio de Moraes Andrade and Jorge Lu?s Azevedo de Armada
We developed a procedure to obtain fibroblasts from bat skin. A small fragment of the ear is removed under ether anesthesia. This material is then cut up into small pieces and cultured in standard cell culture media. Very good quality chromosome preparations for cytogenetic studies are obtained in about three weeks. Secondary .. Read More»
- Genet. Mol. Res. 1(2):
Molecular cytogenetics in metaphase and interphase cells for cancer and genetic research,diagnosis and prognosis. Application in tissue sections and cell suspensions
Authors: Maria M?¼hlmann
As the pioneer among molecular cytogenetics techniques, fluorescence in situ hybridization (FISH) allows identification of specific sequences in a structurally preserved cell, in metaphase or interphase. This technique, based on the complementary double-stranded nature of DNA, hybridizes labeled specific DNA (probe). The probe.. Read More»
- Genet. Mol. Res. 1(2):