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Allele

Human Genetics   Research Article

Development of a polymorphic short tandem repeat locus multiplex system for efficient human identification

Authors: R.G. Rodovalho, E.L. Rodrigues, G.S. Santos, L.M. Cavalcanti,P.R. Lima, A.G. Rodovalho, R.G. Vital, M.A.D. Gigonzac and A.D. da Cruz

This study aimed to develop a short tandem repeat (STR) multiplex system, made up of 22 highly informative STR loci, for application in forensic genetics. The system comprised 21 polymorphic autosomal loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D2S44.. Read More»

Genet. Mol. Res. 16(2):
gmr16029557
DOI:
10.4238/gmr16029557
Human Genetics   Research Article

Genetic diversity of HLA-DRB1 alleles in the Tujia population of Wufeng, Hubei Province, China

Authors: X.P. Qiu, L. Zhang, F.Y. Zeng, Y. Wen, C. Li, L.X. Qiu, D.X. Cheng and X.X. Wu

We established a genetic database by investigating human leukocyte antigen (HLA)-DRB1 allelic frequencies in a disease-association study in the Tujia population in Wufang, Hubei, China. The allele frequencies of the HLA-DRB1 locus in 262 healthy, unrelated Tujia individuals living in the Wufeng region of the Hubei Province wer.. Read More»

Genet. Mol. Res. 13(3):
2014.July.2.5
DOI:
10.4238/2014.July.2.5
Medical Genetics   Research Article

Microsatellites behaving badly: empirical evaluation of genotyping errors and subsequent impacts on population studies

Authors: A.C. Kelly, N.E. Mateus-Pinilla, M. Douglas, M. Douglas, P. Shelton and J. Novakofski

Microsatellites are useful tools for ecological studies because they can be used to discern population structure, dispersal patterns and genetic relationships among individuals. However, they can also yield inaccurate genotypes that, in turn, bias results, promote biological misinterpretations, and create repercussions for pop.. Read More»

Genet. Mol. Res. 10(4):
2011.October.19.1
DOI:
10.4238/2011.October.19.1
Microbial Genetics   Research Article

Allele frequencies of microsatellite loci for genetic characterization of a Sicilian bovine population

Authors: M. Cosenza, S. Reale, T. Lupo, F. Vitale and S. Caracappa

Short tandem repeats are used as an effective method to trace DNA markers in genotyping. Using a standardized kit, we tested 11 microsatellite markers recommended by the International Society for Animal Genetics (ISAG) in a sample of 495 Sicilian cattle. The aim of this study was to investigate the allele frequencies in the Sicilian cattle population to prov.. Read More»

Genet. Mol. Res. 14(1):
2015.January.30.12
DOI:
10.4238/2015.January.30.12
Human Genetics   Research Article

Ovine prion protein genotype frequencies in northwestern China

Authors: C.-L. Zhao, R. Wu, L. Liu, F.-D. Li, X.-L. Zhang, C. Wang, F. Wang, X.-L. Diao, H.-W. Guan, X. Wang and L. Zhou

Anti-scrapie breeding programs have been initiated to screen for scrapie-resistant sheep based on ovine prion protein gene (PRNP) genotypes at codons 136, 154 and 171 in many countries, especially European Union member states. However, investigation of sheep PRNP genotypes is limited in China, despite the large number of sheep.. Read More»

Genet. Mol. Res. 11(2):
dx.doi.org/2012.June.21.2
DOI:
dx.doi.org/10.4238/2012.June.21.2
Human Genetics   Research Article

Characterization of mitochondrial genotypes in the foundation herd of the Canchim beef cattle breed

Authors: S.C. M�©o C.R. Ferreira M.R. Chiaratti F.V. Meirelles L.C.A. Regitano M.M. Alencar P.F. Barbosa

The Canchim (5/8 Charolais + 3/8 Zebu) beef cattle breed was developed at Southeast-Embrapa Cattle to take advantage of hybrid vigor and to combine the higher growth rate and beef quality of Charolais with tropical adaptations of Zebu. The development of three lineages (old, new, and crossbred) has increased its genetic basis... Read More»

Genet. Mol. Res. 8(1):
vol8-1gmr577
DOI:
10.4238/vol8-1gmr577
Medical Genetics   Original Articles

Multiple displacement amplification for preimplantation genetic diagnosis of fragile X syndrome

Authors: H.-S. Lee, M.J. Kim, C.K. Lim, J.W. Cho, I.O. Song and I.S. Kang

Preimplantation genetic diagnosis (PGD) has become an assisted reproductive technique for couples that have genetic risks. Despite the many advantages provided by PGD, there are several problems, including amplification failure, allele drop-out and amplification inefficiency. We evaluated multiple displacement amplification (M.. Read More»

Genet. Mol. Res. 10(4):
2011.November.17.3
DOI:
10.4238/2011.November.17.3
Human Genetics   Research Article

Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction

Authors: F.A. Martins P.C.S. Carneiro C.T. Guimar�£es J.V. Magalh�£es J.E.S. Carneiro C.D. Cruz

The lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, consideri.. Read More»

Genet. Mol. Res. 8(1):
vol8-1gmr585
DOI:
10.4238/vol8-1gmr585
Medical Genetics   Research Article

Allele frequencies of combined DNA index system (CODIS) and non-CODIS short tandem repeat loci in Goi�¡s, Central Brazil

Authors: R.G. Rodovalho, G.S. Santos, L.M. Cavalcanti, B.F.S.M. Moura, E.L. Rodrigues, P.R. Lima, M.A.D. Gigonzac and T.C. Vieira

In studies of human identification, obtaining a high standard of outcomes and satisfactory conclusions are directly related to the use of highly polymorphic molecular markers. In addition to the combined DNA index system (CODIS) group, it is also important to implement non-CODIS markers into the analysis, as they increase the power of discrimination. Duri.. Read More»

Genet. Mol. Res. 14(3):
2015.July.3.6
DOI:
10.4238/2015.July.3.6
Plant Genetics   Research Article

A two-step method for identification of the Chinese glutinous rice Suyunuo, based on ISSR-SCAR and allele-specific markers

Authors: Y.B. Lin, Y.M. Zhang, Y.Y. Hang, M.M. Li, G.C. Zhou, X.L. Shen and X.Q. Sun

Suyunuo is a valuable glutinous rice variety cultivated mainly in the Lake Taihu area of China. Historically, Suyunuo was presented to emperors as a tribute, and, still today, enjoys a great reputation in China. This study aimed to develop a unique, specific molecular marker for the identification of Suyunuo rice. Polymerase c.. Read More»

Genet. Mol. Res. 15(4):
gmr.15048663
DOI:
10.4238/gmr.15048663