Authors: R.G. Rodovalho, E.L. Rodrigues, G.S. Santos, L.M. Cavalcanti,P.R. Lima, A.G. Rodovalho, R.G. Vital, M.A.D. Gigonzac and A.D. da Cruz
This study aimed to develop a short tandem repeat (STR) multiplex system, made up of 22 highly informative STR loci, for application in forensic genetics. The system comprised 21 polymorphic autosomal loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D2S44.. Read More»
Authors: X.P. Qiu, L. Zhang, F.Y. Zeng, Y. Wen, C. Li, L.X. Qiu, D.X. Cheng and X.X. Wu
We established a genetic database by investigating human leukocyte antigen (HLA)-DRB1 allelic frequencies in a disease-association study in the Tujia population in Wufang, Hubei, China. The allele frequencies of the HLA-DRB1 locus in 262 healthy, unrelated Tujia individuals living in the Wufeng region of the Hubei Province wer.. Read More»
Authors: A.C. Kelly, N.E. Mateus-Pinilla, M. Douglas, M. Douglas, P. Shelton and J. Novakofski
Microsatellites are useful tools for ecological studies because they can be used to discern population structure, dispersal patterns and genetic relationships among individuals. However, they can also yield inaccurate genotypes that, in turn, bias results, promote biological misinterpretations, and create repercussions for pop.. Read More»
Authors: M. Cosenza, S. Reale, T. Lupo, F. Vitale and S. Caracappa
Short tandem repeats are used as an effective method to trace DNA markers in genotyping. Using a standardized kit, we tested 11 microsatellite markers recommended by the International Society for Animal Genetics (ISAG) in a sample of 495 Sicilian cattle. The aim of this study was to investigate the allele frequencies in the Sicilian cattle population to prov.. Read More»
Authors: C.-L. Zhao, R. Wu, L. Liu, F.-D. Li, X.-L. Zhang, C. Wang, F. Wang, X.-L. Diao, H.-W. Guan, X. Wang and L. Zhou
Anti-scrapie breeding programs have been initiated to screen for scrapie-resistant sheep based on ovine prion protein gene (PRNP) genotypes at codons 136, 154 and 171 in many countries, especially European Union member states. However, investigation of sheep PRNP genotypes is limited in China, despite the large number of sheep.. Read More»
Authors: S.C. M�©o C.R. Ferreira M.R. Chiaratti F.V. Meirelles L.C.A. Regitano M.M. Alencar P.F. Barbosa
The Canchim (5/8 Charolais + 3/8 Zebu) beef cattle breed was developed at Southeast-Embrapa Cattle to take advantage of hybrid vigor and to combine the higher growth rate and beef quality of Charolais with tropical adaptations of Zebu. The development of three lineages (old, new, and crossbred) has increased its genetic basis... Read More»
Authors: H.-S. Lee, M.J. Kim, C.K. Lim, J.W. Cho, I.O. Song and I.S. Kang
Preimplantation genetic diagnosis (PGD) has become an assisted reproductive technique for couples that have genetic risks. Despite the many advantages provided by PGD, there are several problems, including amplification failure, allele drop-out and amplification inefficiency. We evaluated multiple displacement amplification (M.. Read More»
Authors: F.A. Martins P.C.S. Carneiro C.T. Guimar�£es J.V. Magalh�£es J.E.S. Carneiro C.D. Cruz
The lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, consideri.. Read More»
Authors: R.G. Rodovalho, G.S. Santos, L.M. Cavalcanti, B.F.S.M. Moura, E.L. Rodrigues, P.R. Lima, M.A.D. Gigonzac and T.C. Vieira
In studies of human identification, obtaining a high standard of outcomes and satisfactory conclusions are directly related to the use of highly polymorphic molecular markers. In addition to the combined DNA index system (CODIS) group, it is also important to implement non-CODIS markers into the analysis, as they increase the power of discrimination. Duri.. Read More»
Authors: Y.B. Lin, Y.M. Zhang, Y.Y. Hang, M.M. Li, G.C. Zhou, X.L. Shen and X.Q. Sun
Suyunuo is a valuable glutinous rice variety cultivated mainly in the Lake Taihu area of China. Historically, Suyunuo was presented to emperors as a tribute, and, still today, enjoys a great reputation in China. This study aimed to develop a unique, specific molecular marker for the identification of Suyunuo rice. Polymerase c.. Read More»