Apoptosis
In vitro cytotoxicity screening of wild plant extracts from Saudi Arabia on human breast adenocarcinoma cells
Authors: M.A. Ali, M. Abul Farah, F.M. Al-Hemaid and F.M. Abou-Tarboush
This study investigated the in vitro anticancer activities of a total of 14 wild angiosperms collected in Saudi Arabia. The cytotoxic activity of each extract was assessed against human breast adenocarcinoma (MCF-7) cell lines by using the MTT assay. Among the plants screened, the potential cytotoxic activity exhibited by the .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.9
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.9
Roles of Fas/Fasl, Bcl-2/Bax, and Caspase-8 in rat nonalcoholic fatty liver disease pathogenesis
Authors: C.P. Li, J.H. Li, S.Y. He, P. Li and X.L. Zhong
The aim of this study was to investigate the roles of Fas/FasL, Bcl-2/Bax, and Caspase-8 mRNA expressions in nonalcoholic fatty liver disease (NAFLD). The apoptosis percentage was measured by flow cytometry, the immunohistochemical assay was performed for the determination of Fas, FasL, Bcl-2, and Bax expressions, and a real-t.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.10
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.10
Fas/FasL in the immune pathogenesis of severe aplastic anemia
Authors: C.Y. Liu, R. Fu, H.Q. Wang, L.J. Li, H. Liu, J. Guan, T. Wang, W.W. Qi, E.B. Ruan, W. Qu, G.J. Wang, H. Liu, Y.H. Wu, J. Song, L.M. Xing and Z.H. Shao
Fas/FasL protein expression of bone marrow hematopoietic cells was investigated in severe aplastic anemia (SAA) patients. Fas expression was evaluated in CD34+, GlycoA+, CD33+, and CD14+ cells labeled with monoclonal antibodies in newly diagnosed and remission SAA patients along with normal controls. FasL expression was evalua.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.3
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.3
The role of Hsp90?± in heat-induced apoptosis and cell damage in primary myocardial cell cultures of neonatal rats
Authors: A. Islam, Y.J. Lv, A. Abdelnasir, B. Rehana, Z.J. Liu, M. Zhang S. Tang, Y.F. Cheng, H.B. Chen, J. Hartung, E.D. Bao
To understand the mechanism underlying the sudden animal death caused by acute heart failure during heat stress, the relationships among the heat-induced pathological changes and apoptosis and the variations in the levels of protective Hsp90α and its mRNA in the heat-stressed primary myocardial cells of neonatal rats in .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.2.6
- DOI:
- 10.4238/2013.December.2.6
Protein transduction domain-hA20 fusion protein protects endothelial cells against high glucose-induced injury
Authors: C.L. Hou, Q. Huang, Y. Wei, W. Zhang, J.H. Mi, D.J. Ying and Z.H. Zhou
We constructed a plasmid containing a protein transduction domain (PTD) and a human A20 (hA20) gene fragment; the fusion protein was obtained by highly expressing this plasmid in the yeast Pichia pastoris GS115. The plasmid was obtained by adding 9xArg and EcoRÐ? recognition sites to the end of the primer, and 6xHis-Tag and .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.19.9
- DOI:
- 10.4238/2012.July.19.9
Induction of apoptosis in human cervical carcinoma Hela cells with active components of Menispermum dauricum
Authors: J.Y. Wang, S. Sun, L. Liu and W.S. Yang
Menispermum dauricum DC possesses a wide range of pharmacological effects. In this study, the mechanism of apoptosis induced by active components of M. dauricum was investigated in the human cervical carcinoma HeLa cell line. HeLa cells were treated with different M. dauricum concentrations over different time periods. The pro.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.February.13.17
- DOI:
- 10.4238/2014.February.13.17
Propofol induces apoptosis and inhibits the proliferation of rat embryonic neural stem cells via gamma-aminobutyric acid type A receptor
Authors: J.W. Wang, W.W. Cheng, T. Xu and Z.Y. Yang
We investigated the effect of propofol on the proliferation and viability of rat embryonic neural stem cells (rENSCs) and the potential mechanisms involved. rENSCs were isolated and cultured in vitro and treated with 1, 10, or 50 μM propofol, while the control group was treated with 0.1 μM dimethyl sulfoxide. Th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.57
- DOI:
- 10.4238/2015.November.18.57
miR-34a targets the inhibin beta B gene, promoting granulosa cell apoptosis in the porcine ovary
Authors: F. Tu, Z.X. Pan, Y. Yao, H.L. Liu, S.R. Liu, Z. Xie and Q.F. Li
During ovarian follicular growth and development, only a few follicles actually ovulate. Recently, it was found that follicular atresia is triggered by granulosa cell apoptosis, but the molecular mechanism of follicular atresia was not understood. Using flow cytometry, we found that miR-34a promotes granulosa cell apoptosis in.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.14.6
- DOI:
- 10.4238/2014.January.14.6
Role of death receptors in the regulation of hepatocyte proliferation and apoptosis during rat liver regeneration
Authors: Y. Zhou1,2, J.C. Xu2, Y.F. Jia1 and C.S. Xu1
The balance between hepatocyte proliferation and apoptosis is critical for liver homeostasis during liver regeneration. We created a rat liver regeneration model by partial hepatectomy (PH) to investigate the overall mechanism that regulates the proliferation and apoptosis of hepatocytes. The Rat Genome 230 2.0 Array was used to investigate changes in the.. Read More»
- Genet. Mol. Res. 14(4):
- 0.4238/2015.October.29.26
- DOI:
- 0.4238/2015.October.29.26
Effect of sphingosine-1-phosphate and myoblast transplantation on rat acute myocardial infarction
Authors: H. Yu1, P.L. Chen2, Y. Zhao1, X. Gu3, W. He1 and Z. Gong2
In this study, we investigated the effects of sphingosine-1-phosphate (S1P) combined with myoblast transplantation on the treatment of acute myocardial infarction and provided a foundation for its clinical application. A rat model of acute myocardial infarction was established by ligating the anterior descending branch of the coronary artery. Serum-free m.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.29.4
- DOI:
- 10.4238/2015.October.29.4