Apoptosis
Inflammatory response to isocyanates and onset of genomic instability in cultured human lung fibroblasts
Authors: P.K. Mishra A. Bhargava G.V. Raghuram S. Gupta S. Tiwari R. Upadhyaya S.K. Jain K.K. Maudar
Lungs comprise the primary organ exposed to environmental toxic chemicals, resulting in diverse respiratory ailments and other disorders, including carcinogenesis. Carcinogenesis is a multi-stage phenomenon, which involves a series of genetic alterations that begin with genomic instability provoked by certain factors such as i.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr544
- DOI:
- 10.4238/vol8-1gmr544
Docetaxel enhances apoptosis and G2/M cell cycle arrest by suppressing mitogen-activated protein kinase signaling in human renal clear cell carcinoma
Authors: T.D. Han, D.H. Shang and Y. Tian
Tremendous efforts have been made in renal cell carcinoma (RCC) patients’ research; however, clinical findings in patients have been disappointing. The aims of our study were to identify better or alternative therapeutic methods that can reverse chemotherapy resistance and to enhance sensitivity to docetaxel (DOX)-based chemotherapy drugs. We evalua.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017321
- DOI:
- 10.4238/gmr.15017321
Inhibition of vascular endothelial growth factor A expression in mouse granulosa cells by lentivector-mediated RNAi
Authors: N. Ally X.L. Zou B.C. Jiang L. Qin L. Zhai P. Xiao H.L. Liu
Vascular endothelial growth factor (VEGF) has been found responsible for the induction of proliferation and differentiation in granulosa cells. We constructed four short hairpin RNA (shRNA) expression plasmids targeting the mouse VEGFA gene, and examined their effect on VEGF expression in mouse granulosa cells (MGC) in vitro. Four different shRNA oligonuc.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.28.1
- DOI:
- 10.4238/2012.November.28.1
Roles of osteocyte apoptosis in steroid-induced avascular necrosis of the femoral head
Authors: R. Bai1*, W. Feng2*, W.L. Liu1, Z.H.Q. Zhao1, A.Q. Zhao1, Y. Wang1, W.X. Wang1, L. Sun1, L.S.H. Wu1 and S.H.X. Cui1
An animal model of steroid-induced avascular necrosis of the femoral head (SANFH) was established to investigate the roles of osteocyte apoptosis in this process. Forty five-month-old male and female Japanese white rabbits were randomly divided into groups A (hormone + endotoxin), B (hormone alone), C (endotoxin alone), and D (blank control). Animals were.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017529
- DOI:
- 10.4238/gmr.15017529
Mechanism of juglone-induced apoptosis of MCF-7 cells by the mitochondrial pathway
Authors: Y.B. Ji, G.S. Xin, Z.Y. Qu, X. Zou and M. Yu
This study investigated the nature and mechanism of juglone-induced apoptosis in the human breast cancer cell line MCF-7. The inhibitory effect of juglone on MCF-7 cell growth was evaluated by the dimethylthiazol tetrazolium assay. Morphological apoptotic changes were characterized using an inverted microscope, Hoechst 33258 fluorescence staining, and Gie.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038785
- DOI:
- 10.4238/gmr.15038785
Effect of the extract from leaves of Liquidambar formosana Hance on S180 cells
Authors: Y.T. Zhong, X.L. Wang, Q.J. Xie and Y.N. Zhang
We examined the effects of the extract from leaves of Liquidambar formosana Hance on S180 cells and screened for antitumor active sites in the plant. Solvent extraction was conducted to prepare extracts from the leaves of L. formosana Hance and conduct preliminary separation, an MTT assay to determine the effect of leaf extract on the proliferation of S18.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038795
- DOI:
- 10.4238/gmr.15038795
Expression profiles of genes associated with mitochondria-mediated apoptosis and their roles in liver regeneration
Authors: X.K. Xing, M.H. Li, Z.W. Guo and C.S. Xu
Mitochondria are closely associated with cell survival, and it is of interest to determine whether apoptosis pathways, which are mediated by mitochondria, are involved in liver regeneration (LR). To identify the mechanisms underlying mitochondria-mediated apoptosis during rat LR, we used the Rat Genome 230 2.0 Array to investigate changes in gene expressi.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038101
- DOI:
- 10.4238/gmr.15038101
Molecular cloning and expression analysis of the GNAS gene in pig and porcine fibroblast cells
Authors: J. Peng, Y.L. Li, Y.F. Shen, Y. Wang and N.Y. Xu
The Alpha subunit of the stimulatory guanine nucleotide-binding protein (GNAS) is a complex imprinted gene. The major product of the GNAS gene is the α-subunit of the guanine nucleotide-binding protein (Gas), which plays a key role in multiple signal transduction pathways. Gas is required for the production of the recept.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.26
- DOI:
- 10.4238/2014.July.24.26
Fyn arrests swainsonine-induced apoptosis in 293T cells via Akt and its phosphorylation
Authors: L. An, W.W. Li and G.C. Cheng
Swainsonine (SW), an extract from Astragalus membranaceus, represents a new class of compounds that inhibit growth and induce apoptosis in a cancer model. In this study, we demonstrated the effect of Fyn on SW-induced apoptosis in 293T cells. Western blotting was used to measure the expression of the apoptosis-related factors caspase-3, Bcl-2, Bax, and th.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.18.23
- DOI:
- 10.4238/2015.May.18.23
Effects of docetaxel plus three-dimensional conformal radiation therapy on microvessel density and apoptosis expression in local advanced squamous non-small-cell lung cancer
Authors: X.J. Zhai, H.R. Cheng, H.L. Long, W.K. Mao, L. Cao, B.R. Xiao and R.Q. Li
We examined the effects of weekly single-agent docetaxel plus three-dimensional conformal radiation therapy (3D-CRT) on apoptotic index (AI) and microvessel density (MVD) in local advanced non-small-cell lung squamous cancer patients and analyzed the correlation of MVD, AI, and 50% tumor shrinkage time (T0.5) The molecular mechanism of docetaxel radiosens.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.22.9
- DOI:
- 10.4238/2015.May.22.9