Apoptosis
PUMA gene transfection can enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells
Authors: C.-G. Sun, J. Zhuang, W.-J. Teng, Z. Wang and S.-S. Du
We explored whether p53 upregulated modulator of apoptosis (PUMA) gene transfection could enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells. The liposome-mediated recombinant eukaryotic expression vector PUMA-pCDNA3 and empty vector plasmid were stably transfected into MCF-7 cells. Epirubicin (0.01-100 μM) was applied.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.6
- DOI:
- 10.4238/2015.May.29.6
Effect of Rhizoma paridis total saponins on apoptosis of colorectal cancer cells and imbalance of the JAK/STAT3 molecular pathway induced by IL-6 suppression
Authors: W-J. Teng, P. Chen, F.-Y. Zhu, K. Di, C. Zhou, J. Zhuang,X.-J. Cao, J. Yang, L.-J. Deng and C.-G. Sun
We observed the influence of different concentrations of Rhizoma paridis total saponins (RPTS) on the apoptosis of colorectal cancer cells and explored the internal mechanism involved. We determined whether RPTS influences the interleukin-6 (IL-6)/Janus kinase (JAK)- signal transducer and activator of transcription-3 (STAT3) apoptosis molecular pathway an.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.11
- DOI:
- 10.4238/2015.May.29.11
Effects of intra- and extracellular factors on anti-aging klotho gene expression
Authors: K. Turan and P. Ata
Inactivation of the klotho gene in mice causes serious systemic disorders, resembling human aging. However, at the molecular level, its action mechanisms are not well understood. The stimulatory or inhibitory effects of cis- and trans-regulatory factors on the klotho gene expression are also still unclear. We studied the effec.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1261
- DOI:
- 10.4238/vol10-3gmr1261
Overexpression of the growth arrest-specific homeobox gene Gax inhibits proliferation, migration, cell cycle progression, and apoptosis in serum-induced vascular smooth muscle cells
Authors: H. Zheng, S. Xue, Z.L. Hu, J.G. Shan and W.G. Yang
The Gax gene has been implicated in a variety of cell-developmental and biological processes, and aberrant Gax expression is linked to many diseases. In this study, to provide important insights for Gax-based gene therapy in vein graft restenosis and its anti-restenotic mechanism, we used rabbit vascular smooth muscle cells (V.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.24.4
- DOI:
- 10.4238/2014.March.24.4
microRNA 421 induces apoptosis of c-33a cervical cancer cells via down-regulation of Bcl-xL
Authors: X.J. Lai, X.Y. Cheng and L.D. Hu
Cervical cancer is a life-threatening condition. MicroRNAs (miRNAs) can promote or inhibit cell death and proliferation. The present study investigated the effect of miRNA 421 on the growth and apoptosis of cervical cancer cells. miRNA 421 and control miRNA were synthesized and transfected into c-33a cervical cancer cells. A t.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048853
- DOI:
- 10.4238/gmr15048853
Significance of SODD expression in childhood acute lymphoblastic leukemia and its influence on chemotherapy
Authors: H.F. Tao, Y.S. Liu, J.L. Fang, Y.Z. Su, F.H. Chen, L.Y. Zhou and Y.S. Zhu
This study explored the clinical significance of silencer of death domain (SODD) expression in childhood acute lymphoblastic leukemia (ALL) and its influence on chemotherapy as well as the effect of SODD expression on apoptosis of leukemic cells. The expression of SODD proteins in different ALL groups was determined by immunoc.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.24.6
- DOI:
- 10.4238/2014.March.24.6
Protective effect of icariin on kidney in 5/6 nephrectomized rats and its mechanism
Authors: S.R. Liang, J.W. Bi, Z.L. Guo, Y. Bai and Z. Hu
The aim of this study was to investigate the renal protective effect of icariin in 5/6 nephrectomized rats and the molecular mechanisms involved. Forty male Sprague-Dawley rats were randomly divided into 5 groups: sham-operated group, 5/6 nephrectomy model group, icariin groups (20 and 40 mg/kg), and benazepril group. After 12.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.25.10
- DOI:
- 10.4238/2014.August.25.10
Changes in the expression of FoxO1 and death ligand genes during follicular atresia in porcine ovary
Authors: F. Lin, Y.H. Fu, J. Han, M. Shen, C.W. Du, R. Li, X.S. Ma and H.L. Liu
Follicular atresia, a key phenomenon in follicle development, eliminates most of the follicles in mammalian ovaries. To investigate the molecular mechanism of follicular atresia in porcine ovaries, we investigated the mRNA expression of three important cell death ligand-receptor systems and Fox O1 in follicles with a diameter .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.8
- DOI:
- 10.4238/2014.August.28.8
Zinc finger protein A20 overexpression inhibits monocyte homing and protects endothelial cells from injury induced by high glucose
Authors: C.L. Hou, W. Zhang, Y. Wei, J.H. Mi, L. Li, Z.H. Zhou, W. Zeng and D.J. Ying
Diabetes mellitus causes vascular lesions and may ultimately lead to atherosclerosis. One of the earliest steps in the development of atherosclerotic lesions is the adhesion of monocytes to endothelial cells of the vessel wall. It is currently unknown whether zinc finger protein A20 is able to protect endothelial cells from in.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1102
- DOI:
- 10.4238/vol10-2gmr1102
Inhibitory effect of survivin-targeting small interfering RNA on gastric cancer cells
Authors: Y.H. Li, M. Chen, M. Zhang, X.Q. Zhang, S. Zhang, C.G. Yu, Z.M. Xu and X.P. Zou
A pair of inverted repeated sequences of the gene survivin was designed for stable double-stranded RNA establishment. After stable transfection, the biological behaviors of gastric cancer cells were observed. The interference rates of survivin-targeting siRNA (siRNA-survivin) in BGC823, MKN45, SGC7901, and ci.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.22
- DOI:
- 10.4238/2014.August.28.22