Arabidopsis Thaliana
Expression of recombinant human anti-TNF-�± scFv-Fc in Arabidopsis thaliana seeds
Authors: N. Yao, L. Ai, Y.Y. Dong, X.M. Liu, D.Z. Wang, N. Wang, X.W. Li,F.W. Wang, XK. Li, H.Y. Li and C. Jiang
Recombinant human anti-tumor necrosis factor (TNF)-α scFv-Fc was expressed in TKO mutant Arabidopsis thaliana seeds using plant-specific codons. Immunoblotting using a human IgG1 antibody detected the expression of anti-TNF-α proteins in plants. Results from qRT-PCR analysis demonstrated that the time of harvest si.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027726
- DOI:
- 10.4238/gmr.15027726
An efficient method for simultaneous extraction of high-quality RNA and DNA from various plant tissues
Authors: R.R. Oliveira, A.J.C. Viana, A.C.E. Re�¡tegui and M.G.A. Vincentz
Determination of gene expression is an important tool to study biological processes and relies on the quality of the extracted RNA. Changes in gene expression profiles may be directly related to mutations in regulatory DNA sequences or alterations in DNA cytosine methylation, which is an epigenetic mark. Correlation of gene expression with DNA sequence or.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.32
- DOI:
- 10.4238/2015.December.28.32