Authors: F.E. Porto, A.L.B. Portela-Castro and I.C. Martins-Santos
We made a cytogenetic study of Rineloricaria pentamaculata from the Tauá Stream, in the Pirapó River sub-basin in Paraná State, Brazil, focused on the occurrence and origins of the B chromosomes. The diploid number varied from 2n = 56 to 2n = 59, due to the presence of 0 to 3 B microchromosomes of the acro.. Read More»
Authors: Fuzinatto VA, Pagliarini MS and Valle CB
Morphological changes have been investigated during plant programmed cell death (PCD) in the last few years due to the new interest in a possible apoptotic-like phenomenon existing in plants. Although PCD has been reported in several tissues and specialized cells in plants, there have been few reports of its occurrence during .. Read More»
Authors: J.M.P. Cordeiro, S.A.A. Lima, S.N. Paz, A.M.S. Santos and L.P. Felix
Most taxa in the Bignoniaceae have 2n = 40, but the basal clade Jacarandeae has 2n = 36, suggesting that x = 18 is the ancestral basic number for the family. Variations in heterochromatin band patterns in genera that are numerically stable, such as Jacaranda, could facilitate our understanding of the chromosomal and karyotypic.. Read More»
Authors: L.B. Ribeiro, D.A. Matoso, M.C. Almeida, M.R. Vicari,A. Moraes-Neto, M.C.C.M. Svidnicki and R.F. Artoni
Cytogenetic analyses were carried out in a populational sample of Iheringichthys labrosus from the Guaraúna River (Upper Tibagi River; Paraná State, Brazil) in order to provide a karyotypic comparison with another previously studied population from the Lower Tibagi River, characterized by the presence of 32m + 8s.. Read More»
Authors: Mariela Nieves, Mar�?a M�?¼hlmann and Marta Dolores Mudry
Neotropical Primate karyotypes are highly variable, particularly in the heterochromatic regions, not only regarding the amount of heterochromatin, but also the composition. G and C banding and FISH techniques provide useful information to characterize interspecific relationships. We used chromosome microdissection to develop a.. Read More»
Authors: Kleber Agari Campos and Carmem Silvia Fontanetti
Triple staining with fluorochromes (DA/DAPI/CMA) and C-banding were used to characterize the composition of Pseudonannolene strinatii heterochromatin. C-banding showed C+ bands of different labeling intensity on chromosomes 1 and 2 in some cells. Fluorochrome staining revealed DAPI+ regions corresponding to the C-banding patte.. Read More»
Authors: K.C.C. Alevi, L.A.C Rodas, E. Tartarotti, M.T.V. Azeredo-Oliveira and M.M. Guirado
To complement the epidemiological data and assist in the prophylaxis of Chagas disease in the State of São Paulo, we examined entomological lifting conducted in 40 municipalities of the Western region of the state from 2004 to 2008, highlighted the main vector species in this region, and reanalyzed the cytogenetic characteristics of Rhodnius neglec.. Read More»
Authors: Y. Sun, C.H. Xu, M.Q. Wang, D.Y. Zhi and G.M. Xia
A broad spectrum of genetic and epigenetic changes is induced by wide hybridization and subsequent polyploidization, but the timing of these events remains obscure because early hybrid cells are very difficult to harvest and analyze. Here, we used both cytological and genetic marker approaches to analyze the constitution of very young somatic hybrid cells.. Read More»
Authors: K.-E. Lee and H.-S. Park
We analyzed the publicly available ChromHMM BED files of the ENCODE project and tested the Markov properties of the different chromatin states in the human genome. Nucleotide frequency profiles of regional chromatin segmentations were analyzed, and Markov chains were built to detect Markov properties in the chromatin states of different ChromHMM regions. .. Read More»
Authors: G.J. Chang, H.M. Seyfert and X.Z. Shen
In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing th.. Read More»