Cloning
Comparing KISS1 and GPR54 genes between the two breeds of goats with low and high prolificacy
Authors: Jie Zheng, Sayed Haidar Abbas Raza, Xiang-Dong Zi, Jian-Yuan Lu
There is increasing evidence that kisspeptin, the peptide product of KISS1 gene and its receptor (GPR54), regulate reproductive processes through actions on other organs than hypothalamus. Therefore, the study for the first time, was conducted to investigate the nucleotide sequences and mRNA expression levels of KISS1 and GPR54 genes in pituitary, endomet.. Read More»
- Genet. Mol. Res. 17(4):
- http://dx.doi.org/gmr16039922
- DOI:
- http://dx.doi.org/10.4238/gmr16039922
Cloning and expression analysis of pistillata-like gene in Sedirea japonica
Authors: Suhua Jiang, Suyan Niu, Xiuyun Yuan, Fang Liang, Shenping Xu, Mofei Wang, Bo Cui
Members of the pistillata-like (PI-like) subfamily of plant MADS-box genes play multiple roles in regulation of reproductive meristems and the specification of sepal and petal identities. However, the function of PI-like genes in the Sedirea japonica is largely unknown. In this study, a full-length of PI-like gene in Sedirea j.. Read More»
- Genet. Mol. Res. 17(1):
- gmr16039855
- DOI:
- 10.4238/gmr16039855
Cloning and functional characterization of the HbSYR1 gene encoding a syntaxin-related protein in Tibetan hulless barley (Hordeum vulgare L. var. nudum HK. f.)
Authors: Q.J. Xu, Y.L. Wang, Z.X. Wei, H.J. Yuan, X.Q. Zeng and N. Tashi
Tibetan barley is a staple food for the natives of Qinghai-Tibet Plateau. Drought causes a reduction in barley production. In this study, the full-length cDNA of a gene encoding a syntaxin-associated protein was cloned from the leaves of a drought-resistant variety of barley, “Himalaya 10”; its expression was evalu.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16038909
- DOI:
- 10.4238/gmr16038909
DNA barcoding for species identification in the Palmae family
Authors: A. Naeem1, A.A. Khan1, H.M.N. Cheema1, I.A. Khan1 and A. Buerkert2
DNA barcoding is a promising tool for species identification at the molecular level. The barcoding system is well established for species differentiation in animals, while it is less common in plants. We evaluated 2 barcoding regions, maturase K (matK) and ribulose bisphosphate carboxylase (rbcL), to compare species of Palmae according to amplification su.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.29
- DOI:
- 10.4238/2014.December.4.29
Molecular characterization of the pseudorabies virus UL2 gene
Authors: M.S. Cai1,2, B.Y. Wang2, W. Cui1, Z.Y. Zhao1, J.H. Chen2, X.M. Wen1, Z. Li1 and M.L. Li1
A 948-bp sequence of the UL2 gene was amplified from the pseudorabies virus (PRV) Becker strain genome using polymerase chain reaction, and the gene identity was confirmed through further cloning and sequencing. Bioinformatic analysis indicated that the PRV UL2 gene encodes a putative polypeptide with 315-amino acid residues. Its encoding protein, designa.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.7.1
- DOI:
- 10.4238/2013.October.7.1
Cloning, characterization, and expression of the macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (Penaeidae)
Authors: D.G. Zeng, A.Y. Lei, X.H. Chen
The macrophage migration inhibitory factor (MIF) is an important proinflammatory cytokine that mediates both innate and adaptive immune responses. In this study, we identified a homolog of MIF in the Pacific white shrimp Litopenaeus vannamei. The MIF cDNA contained a 363-bp open reading frame encoding a 120-amino acid protein .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.22.15
- DOI:
- 10.4238/2013.November.22.15
Molecular cloning and expression of the porcine S14R gene in Escherichia coli
Authors: Y.J. Guo1*, G.Z. Liu1*, C.M. Wang1, Y.Y. Wang2, H.J. Li2, K. Zhong2, W.F. Lu2, Y.L. Wang2, G.Y. Yang2
We amplified S14R protein gene cDNA of porcine, cloned it into a prokaryotic expression plasmid, and expressed it in Escherichia coli. A pair of primers was designed based on the cDNA sequence of the porcine S14R gene in GenBank. The target gene fragment from porcine liver tissue was amplified by RT-PCR. Confirmed by auto-sequencing, the target gene fragm.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.10.6
- DOI:
- 10.4238/2013.October.10.6
Full-length cDNA cloning and structural characterization of preproinsulin in Alligator sinensis
Authors: R. Zhang, S.Z. Zhang, E. Li, C. Wang, C.L. Wang and X.B. Wu
Insulin is an important endocrine hormone that plays a critical physiological role in regulating metabolism and glucostasis in vertebrates. In this study, the complete cDNA of Alligator sinensis preproinsulin gene was cloned for the first time by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods; the amino acid s.. Read More»
- Genet. Mol. Res. 13(4):
Identification of spliced mRNA isoforms of retinoid X receptor (RXR) in the Oriental freshwater prawn Macrobrachium nipponense
Authors: Z. Li, W.Q. Wang, E.F. Zhang and G.F. Qiu
Retinoid X receptors (RXR) are members of the nuclear receptor family that are conserved from invertebrates to vertebrates, and they play an essential role in regulating reproductive maturation, molting, and embryo development. In this study, fi ve RXR isoforms, named RXRL2 (L, long form), RXRL3, RXRS1 (S, short form), RXRS2, .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.2
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.2
Overexpression, purification, and pharmacologic evaluation of anticancer activity of ribosomal protein L24 from the giant panda (Ailuropoda melanoleuca)
Authors: Y.L. Hou, X. Ding, W. Hou, B. Song, T. Wang, F. Wang, J. Li, J. Zhong, T. Xu, B.X. Ma, H.Q. Zhu, J.H. Li and J.C. Zhong
The ribosomal protein L24 (RPL24) belongs to the L24E family of ribosomal proteins and is located in the cytoplasm. The purpose of this study was to investigate the structure and anti-cancer function of RPL24 of the giant panda (Ailuropoda melanoleuca). The complementary DNA of RPL24 was cloned successfully using reverse transcription-polymerase chain rea.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.11
- DOI:
- 10.4238/2013.October.18.11