Cloning
Ectopic expression of the BoTFL1-like gene of Bambusa oldhamii delays blossoming in Arabidopsis thaliana and rescues the tfl1 mutant phenotype
Authors: H.Y. Zeng, Y.T. Lu, X.M. Yang, Y.H. Xu and X.C. Lin
TERMINAL FLOWER1 (TFL1) homologous genes play major roles in maintaining vegetative growth and inflorescence meristem characteristics in various plant species; however, to date, the function of the bamboo TFL1 homologous gene has not been described. In this study, a TFL1 homologous gene was isolated from Bambusa oldhamii and designated as BoTFL1-like. Phy.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.10.11
- DOI:
- 10.4238/2015.August.10.11
Cloning and transformation analysis of isoflavone synthase gene into Minshan Trifolium pratense
Authors: H.H. Hu, C.Q. Jing, R. Liu, W.D. Li and H.G. Feng
The aim of this study was to clone the isoflavone synthase (IFS) gene and establish the recombinant Minshan Trifolium pratense. The IFS gene was cloned from the callus of Minshan T. pratense using reverse transcription-polymerase chain reaction. The plant expression vector pRI101-AN-IFS was constructed and introduced into Agrobacterium tumefaciens strain .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.10.9
- DOI:
- 10.4238/2015.August.10.9
cDNA, genomic sequence cloning and overexpression of the ribosomal protein S13 gene in the giant panda (Ailuropoda melanoleuca)
Authors: Y. Song, Y.-L. Hou, W.-R. Hou, G.-F. Wu and T. Zhang
The cDNA and the genomic sequence of ribosomal protein S13 (RPS13) of the giant panda (Ailuropoda melanoleuca) was cloned using reverse transcription-polymerase chain reaction (RT-PCR) and touchdown-PCR, respectively. These two sequences were sequenced and analyzed, and the cDNA of the RPS13 gene was overexpressed in Escherich.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1000
- DOI:
- 10.4238/vol10-1gmr1000
Cloning and characterization of the nicotianamine synthase gene in Eruca vesicaria subsp sativa
Authors: B.L. Huang, C. Cheng, G.Y. Zhang, J.J. Su, Y. Zhi, S.S. Xu, D.T. Cai, X.K. Zhang and B.Q. Huang
Nicotianamine (NA) is a ubiquitous metabolite in plants that bind heavy metals, is crucial for metal homeostasis, and is also an important metal chelator that facilitates long-distance metal transport and sequestration. NA synthesis is catalyzed by the enzyme nicotianamine synthase (NAS). Eruca vesicaria subsp sativa is highly tolerant to Ni, Pb, and Zn. .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.38
- DOI:
- 10.4238/2015.December.22.38
Cloning of superoxide dismutase from post-harvest Hami melon and quantitative expression analysis before and after disease
Authors: C.H. Shan, F.X. Tang, W. Chen and W.R. Ma
Primers were designed according to the Cu/Zn-SOD gene sequences of cloned Cucurbits plants (cucumbers and watermelons) available in NCBI. Total RNA from Hami melon pulp was used as a template. Following RT-PCR amplification, a 403-bp fragment of the Hami melon Cu/Zn-SOD gene was obtained. According to alignment in BLAST and phylogenetic tree analysis, the.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.10
- DOI:
- 10.4238/2015.December.23.10
Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana
Authors: W.Y. Xuan, Y. Zhang, Z.Q. Liu, D. Feng and M.Y. Luo
Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. m.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.27
- DOI:
- 10.4238/2015.August.19.27
Cloning and characterization of ADP-glucose pyrophosphorylase small subunit gene in Cyperus esculentus (yellow nutsedge)
Authors: C. Cheng, J. Hu, Y. Zhi, J.J. Su, X.K. Zhang and B.Q. Huang
ADP-glucose pyrophosphorylase (ADPGlcPPase) controls the first committed step of starch synthesis by catalyzing the biosynthesis of ADP-glucose from glucose-phosphate and ATP. It is a tetrameric protein consisting of two small and two large subunits. The small subunits have a catalytic function, while the large subunits regulate the enzyme activity. Cyper.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.18
- DOI:
- 10.4238/2015.December.23.18
Molecular cloning and expression analysis of a pearl oyster (Pinctada martensii) heat shock protein 90 (HSP90)
Authors: H.Y. Liang, Z.X. Wang, Q.N. Lei, R.L. Huang, Y.W. Deng, Q.H. Wang, Y. Jiao and X.D. Du
Heat shock protein 90 (HSP90) is an important molecular chaperone required for proper folding of cellular proteins, and thus, it plays an essential role in protecting cells from damage during stress. In this study, an HSP90 cDNA designated PmHSP90 was cloned from the mantle tissue of the pearl oyster Pinctada martensii using reverse transcription polymera.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.27
- DOI:
- 10.4238/2015.December.28.27
Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii
Authors: Q.N. Lei, Y.Y. Wu, H.Y. Liang, Z.X. Wang, Z. Zheng and Y.W. Deng
Small heat shock proteins (HSPs) are molecular chaperones with ATP-independent properties. They are involved in a variety of physiological and stress processes. In this study, the full-length HSP 20 (HSP20) from Pinctada martensii, designated as PmHSP20, was obtained from hemocytes using rapid amplification of cDNA ends techno.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028799
- DOI:
- 10.4238/gmr.15028799
Molecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense
Authors: W.Y. Zhang, S.F. Jiang, Y.W. Xiong, H.T. Fu1,, H. Qiao, S.M. Sun, Y.S. Gong and S.B. Jin
The gene female sterile homeotic (fsh) plays crucial roles in molecular function, including protein kinase activity and DNA binding, which are involved in biological processes such as terminal region determination and negative regulation of DNA-dependent transcription. Although fsh has been found in Drosophila melanogaster, little is known regarding its e.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.30.4
- DOI:
- http://dx.doi.org/10.4238/2015.April.30.4