Cloning
Study on the treatment of the p15 gene combined with Bcr-abl-specific siRNA and STI571 for chronic myeloid leukemia
Authors: W. Wang, Y. Du, N.-N. Li, F.-F. Lv and G.-Q. Lin
The aim of this study was to investigate the effect of the p15 gene combined with Bcr-abl-specific siRNA and STI571 on the proliferation, cell cycle and apoptosis of K562 chronic myeloid leukemia cells. Using the gene sequence results, we amplified the p15 gene from normal peripheral blood by RT-PCR, and constructed a p15-pcDN.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.4
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.4
Identification and authentication of Rosa species through development of species-specific SCAR marker(s)
Authors: K.M.I. Bashir, F.S. Awan, I.A. Khan, A.I. Khan and M. Usman
Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the i.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.8
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.8
Cloning and characterization of major histocompatibility complex class II genes in the stone flounder Kareius bicoloratus (Pleuronectidae)
Authors: Cloning and characterization of major histocompatibility complex class II genes in the stone flounder Kareius bicoloratus (Pleuronectidae)
Major histocompatibility complex (MHC) class II genes play important recognition roles in the immune system in vertebrates. We cloned the MHC class II genes A and B in the stone flounder (Kareius bicoloratus). The full-length cDNA and DNA sequences of both genes were obtained, and their characteristic motifs were analyzed. The DNA sequence of stone flound.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.22.9
- DOI:
- 10.4238/2013.November.22.9
Analysis of an â??off-ladderâ? allele at the Penta D short tandem repeat locus
Authors: Y.L. Yang, J.G. Wang, D.X. Wang, W.Y. Zhang, X.J. Liu, J. Cao and S.L. Yang
Kinship testing of a father and his son from Guangxi, China, the location of the Zhuang minority people, was performed using the PowerPlex® 18D System with a short tandem repeat typing kit. The results indicated that both the father and his son had an off-ladder allele at the Penta D locus, with a genetic size larger than .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.18
- DOI:
- 10.4238/2015.November.24.18
Molecular cloning and expression of the male sterility-related CtYABBY1 gene in flowering Chinese cabbage (Brassica campestris L. ssp chinensis var. parachinensis)
Authors: X.L. Zhang and L.G. Zhang
Expression of the YABBY gene family in the abaxial surface of lateral plant organs determines abaxial destiny of cells, enhances growth and expansion of lateral organs, and plays an important role in polar establishment of lateral organs. However, the YABBY gene has not been studied in male sterility and fertility restoration... Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.10.1
- DOI:
- http://dx.doi.org/10.4238/2014.June.10.1
Cloning and characterization of up-regulated HbSINA4 gene induced by drought stress in Tibetan hulless barley
Authors: H.J. Yuan, X.M. Luo, T.S. Nyima, Y.L. Wang, Q.J. Xu and X.Q. Zeng
Hulless barley is an important crop cereal in Tibetan, China. Drought is a major abiotic stress in barley production. In this study, we cloned the drought-related HbSINA4 gene from the variety ‘Himalaya 10’ and analyzed its expression patterns under different drought and rehydration conditions. The cDNA of.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.30.7
- DOI:
- 10.4238/2015.November.30.7
Cloning, identification, and bioinformatics analysis of a putative aquaporin TsAQP from Trichinella spiralis
Authors: J.M. Cui, N.Z. Zhang, W.H. Li, H.B. Yan and B.Q. Fu
Vaccination as a preventative strategy against Trichinella spiralis infection is an ongoing effort, although no ideal vaccine candidates have been identified until now. Identification of more effective antigens that have a role in essential life stages of the parasite and that may be effective vaccine candidates is therefore o.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.14
- DOI:
- 10.4238/2015.October.19.14
Cloning and expression of the sucrose transporter gene PsSUT1 from tree peony leaf
Authors: Y.H. Li, T. Guo, Y. Cui , Y. Li and D. He
This study reports the cloning of a sucrose transporter gene, PsSUT1, from the leaf of tree peony (Paeonia suffruticosa Lind. cv ‘Huhong’). Expression patterns were examined in different organs and at different developmental stages. The full-length cDNA of PsSUT1 consisted of a 2001-bp sequence containing a 1557-bp.. Read More»
- Genet. Mol. Res. 14(4):
- Y.H. Li*, T. Guo*, Y. Cui , Y. Li and D. He
- DOI:
- Y.H. Li*, T. Guo*, Y. Cui , Y. Li and D. He
Molecular cloning, characterization and expression analysis of WAG-1 in the pistillody line of common wheat
Authors: Q.H. Wang, Z.J. Yang, S.H. Wei, Z.Y. Jiang, Y.F. Yang, Z.S. Hu,Q.X. Sun and Z.S. Peng
Wheat WAG-1 is a C-class MADS-box gene, which is orthologous to AGAMOUS in Arabidopsis. In this study, we report the cloning, characterization, and expression patterns of WAG-1 in the pistillody mutant HTS-1 and its sib-line CSTP. The cDNA of WAG-1 was found to be 765 bp in length, which was equal to the length of its open rea.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.16.12
- DOI:
- 10.4238/2015.October.16.12
Molecular characterization and expression patterns of insulin-like growth factor-binding protein genes in postnatal Nanjiang brown goats
Authors: S.Y. Zhan, L. Chen, L. Li, L.J. Wang, T. Zhong and H.P. Zhang
Insulin-like growth factor-binding proteins (IGFBPs) play a key role in modulating insulin-like growth factors (IGFs), and are considered candidate genes for growth traits in livestock. In this study, we identified the complete coding sequences of IGFBP-1 to -6 in the Nanjiang brown goat, and assessed gene tissue expression pa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.16.22
- DOI:
- 10.4238/2015.October.16.22