Cloning
Restriction-ligation-free (RLF) cloning: a high-throughput cloning method by in vivo homologous recombination of PCR products
Authors: Y. Wang, Y. Liu, J. Chen, M.J. Tang, S.L. Zhang, L.N. Wei, C.H. Li and D.B. Wei
In this study, we optimized a restriction-ligation-free (RLF) method to save time and cost of constructing multiple plasmids with the same gene insert, and examined the efficacy of RLF on high-throughput multi-plasmid cloning. This method utilizes the precise DNA repair and recombination systems within Escherichia coli, which .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.19
- DOI:
- 10.4238/2015.October.9.19
Cloning and sequence analysis of the coding sequence of �?²-actin cDNA from the Chinese alligator and suitable internal reference primers from the �?²-actin gene
Authors: H.N. Zhu, S.Z. Zhang, Y.K. Zhou, C.L. Wang and X.B. Wu
β-Actin is an essential component of the cytoskeleton and is stably expressed in various tissues of animals, thus, it is commonly used as an internal reference for gene expression studies. In this study, a 1731-bp fragment of β-actin cDNA from Alligator sinensis was obtained using the homology cloning technique. Sequ.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.4
- DOI:
- 10.4238/2015.October.9.4
Cloning and prokaryotic expression of the porcine lipasin gene..
Authors: M.M. Li* J. Geng, Y.J. Guo, X.Q. Jiao, W.F. Lu, H.S. Zhu, Y.Y. Wang and G.Y. Yang
Lipasin has recently been demonstrated to be involved in lipid metabolism. In this study, two specific primers were used to amplify the lipasin open reading frame from porcine liver tissue. The polymerase chain reaction product was cloned to a pGEM®-T Easy Vector, digested by SalI and NotI, and sequenced. The lipasin fragment was then cloned to a pET2.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.34
- DOI:
- 10.4238/2015.November.18.34
Identification and bioinformatic analysis of a putative calcium-dependent protein kinase (CDPK6) from Toxoplasma gondii
Authors: N.Z. Zhang, S.Y. Huang, D.H. Zhou, Y. Xu, J.J. He and X.Q. Zhu
Toxoplasma gondii is recognized as an opportunistic human pathogen with a worldwide distribution. Development of effective vaccines is considered the only ideal way to control T. gondii infection. However, only one live vaccine is commercially available for use in sheep and goats. Therefore, the identification of more effective antigenic proteins is very .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.9
- DOI:
- 10.4238/2014.December.18.9
Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer
Authors: A.R. Ferreira, L.F.C. Aguiar Filho, R.V. Sousa, R. Sartori and M.M. Franco
Somatic cell nuclear transfer (SCNT) may affect epigenetic mechanisms and alter the expression of genes related to embryo development and X chromosome inactivation (XCI). We characterized allele-specific expression of the X-linked gene monoamine oxidase type A (MAO-A) in the trophectoderm (TF) of embryos produced by SCNT. Tota.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.26
- DOI:
- 10.4238/2015.October.5.26
Molecular characterization, tissue expression profile and SNP analysis of porcine GLP2R
Authors: Y.L. Chai, H.M. Ma and J. Jiang
Glucagon-like peptide-2 receptor (GLP2R), a member of the G-protein-coupled receptor family, plays an important role in intramuscular fat formation. Little is known, however, about porcine GLP2R. In the present study, GLP2R was cloned, and its expression in pig muscle characterized. By rapid amplification of cDNA ends, gene sequence was obtained from Shaz.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.14
- DOI:
- 10.4238/2015.October.21.14
Cloning and sequencing of the rDNA gene family of the water buffalo (Bubalus bubalis)
Authors: C.Y. Pang T.X. Deng D.S. Tang C.Y. Yang H. Jiang B.Z. Yang X.W. Liang
The rDNA genes coding for ribosomal RNA in animals are complicated repeat sequences with high GC content. We amplified water buffalo rDNA gene sequences with the long and accurate (LA) PCR method, using LA Taq DNA polymerase and GC buffer, based on bioinformatic analysis of related organisms. The rDNA genes were found to consist of 9016 nucleotides, inclu.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.3
- DOI:
- 10.4238/2012.July.10.3
Molecular cloning, sequencing, and expression of Eimeria tenella HSP70 partial gene
Authors: A.L.G. Bogado, G.F. Martins, J.P. Sasse, J. da S. Guimar�?£es Jr., J.L. Garcia
Members of the Eimeria genus are protozoan parasites of the subphylum Apicomplexa (Eimeriidae family), and belong to the coccidia group. Eimeria tenella is one of the most pathogenic species owing to its ability to penetrate the mucosa, and cause inflammation and damage. It is an obligate intracellular parasite that causes dis.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16018964
- DOI:
- 10.4238/gmr16018964
Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
Authors: N.B. Stafuzza C.A. Abbey C.A. Gill J.E. Womack M.E.J. Amaral
River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC li.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.22.6
- DOI:
- 10.4238/2012.May.22.6
Molecular cloning, sequence characterization of a novel pepper gene NADP-ICDH and its effect on cytoplasmic male sterility
Authors: M.H. Deng J.F. Wen J.L. Huo H.S. Zhu X.Z. Dai Z.Q. Zhang H. Zhou X.X. Zou
NADP-dependent isocitrate dehydrogenase (NADP-ICDH) is an important enzyme involved in energy metabolism. The complete coding sequence of the pepper (Capsicum annuum) NADP-ICDH gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. Nuc.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.18.15
- DOI:
- 10.4238/2012.May.18.15